Dr Konrad Büssow

Research Group Leader

Konrad Büssow studied Biochemistry at Freie Universität Berlin and completed his PhD in Biochemistry at the Max Planck Institute for Molecular Genetics in Berlin under the supervision of Professor H. Lehrach, graduating in 1998. He is an experienced biochemist specializing in protein chemistry, biotechnological protein production, and protein crystallography.

Currently he is a researcher in the Department “Structure and Function of Proteins”, headed by Prof. Dr. Wulf Blankenfeldt. He is also head of the technology platform “Recombinant Protein Expression”.

Konrad Büssow has been working since 2007 as a Researcher at the Helmholtz Centre for Infection Research in Braunschweig, Germany. During this period, he has made significant contributions to multiple research projects. He developed a novel system for establishing producer cell lines that incorporates a fluorescent protein marker, cell sorting, and site-specific recombination techniques. His work has also focused on producing glycosylated proteins through cell culture for biophysical studies and X-ray crystallography. Among his achievements, he determined the first structure of a lysosome associated membrane protein (LAMP-3). His more recent work has involved structure determination and inhibitor development for cis-aconitate decarboxylase (ACOD1).

Publications

Zhao M., Chen C., Blankenfeldt W., Pessler F., Büssow K. (2025)

Effect of pH and buffer on substrate binding and catalysis by cis-aconitate decarboxylase

Scientific Reports, 15 (1)

DOI: 10.1038/s41598-025-89341-1 HZI-Repositorium PubMed

Siddique M.N.A.A., Kellermeier F., Ölke M., Zhao M., Büssow K., Oefner P.J., Lührmann A., ... , Dettmer K., Lang R. (2024)

Divergent effects of itaconate isomers on Coxiella burnetii growth in macrophages and in axenic culture

Frontiers in Immunology, 15

DOI: 10.3389/fimmu.2024.1427457 HZI-Repositorium PubMed

Schwanke H., Goncalves Magalhäes V, Schmelz S., Wyler E., Hennig T., Günther T., Grundhoff A., Dölken L., Landthaler M., Van Ham M., Jänsch L., Büssow K., van den Heuvel J., Blankenfeldt W., Friedel C.C., ... , Erhard F., Brinkmann M.M. (2023)

The Cytomegalovirus M35 Protein Directly Binds to the Interferon-b[beta] Enhancer and Modulates Transcription of Ifnb1 and Other IRF3-Driven Genes

J.Virol., 97 (6)

DOI: 10.1128/jvi.00400-23 HZI-Repositorium PubMed

Chen F., Yalcin I., Zhao M., Chen C., Blankenfeldt W., ... , Pessler F., Büssow K. (2023)

Amino acid positions near the active site determine the reduced activity of human ACOD1 compared to murine ACOD1

Sci.Rep., 13 (1)

DOI: 10.1038/s41598-023-37373-w HZI-Repositorium PubMed

Chen F., Elgaher W.A.M., Winterhoff M., Büssow K., Waqas F.H., Graner E., Pires-Afonso Y., Casares Perez L., La Vega L., Sahini N., Czichon L., Zobl W., Zillinger T., Shehata M., Pleschka S., Bähre H., Falk C., Michelucci A., Schuchardt S., Blankenfeldt W., ... , Hirsch A.K.H., Pessler F. (2022)

Citraconate inhibits ACOD1 (IRG1) catalysis, reduces interferon responses and oxidative stress, and modulates inflammation and cell metabolism

Nat.Metab, 4 (5)

DOI: 10.1038/s42255-022-00577-x HZI-Repositorium PubMed

Scheithauer L., Thiem S., Schmelz S., Dellmann A., Büssow K., Brouwer R.M.H.J., Ünal C.M., ... , Blankenfeldt W., Steinert M. (2021)

Zinc metalloprotease ProA of Legionella pneumophila increases alveolar septal thickness in human lung tissue explants by collagen IV degradation

Cell Microbiol.

DOI: 10.1111/cmi.13313 HZI-Repositorium PubMed

Chen F., Lukat P., Iqbal A.A., Saile K., Kaever V., van den Heuvel J., Blankenfeldt W., ... , Büssow K., Pessler F. (2019)

Crystal structure of cis-aconitate decarboxylase reveals the impact of naturally occurring human mutations on itaconate synthesis

Proc.Natl.Acad.Sci.U.S.A, 116 (41)

DOI: 10.1073/pnas.1908770116 HZI-Repositorium PubMed

Büssow K., Themann P., Luu S., Pentrowski P., Harting C., Majewski M., Vollmer V., Köster M., Grashoff M., Zawatzky R., van den Heuvel J., ... , Kröger A., Böldicke T. (2019)

ER intrabody-mediated inhibition of interferon a secretion by mouse macrophages and dendritic cells

PLoS ONE, 14 (4)

DOI: 10.1371/journal.pone.0215062 HZI-Repositorium PubMed

Muthukumar Y., Münkemer J., Mathieu D., Richter C., Schwalbe H., Steinmetz H., Kessler W., Reichelt J., Beutling U., Frank R., Bussow K., van den Heuvel J., Brönstrup M., Taylor R.E., ... , Laschat S., Sasse F. (2018)

Investigations on the mode of action of gephyronic acid, an inhibitor of eukaryotic protein translation from myxobacteria

PLoS One., 13 (7)

DOI: 10.1371/journal.pone.0201605 HZI-Repositorium

Baser B., Spehr J., Bussow K., van den Heuvel J. (2016)

A method for specifically targeting two independent genomic integration sites for co-expression of genes in CHO cells

Methods, 95

DOI: 10.1016/j.ymeth.2015.11.022

Büssow K. (2015)

Stable mammalian producer cell lines for structural biology

Curr.Opin.Struct.Biol., 32

DOI: 10.1016/j.sbi.2015.03.002 HZI-Repositorium PubMed

Jäger Volker, Büssow Konrad, Wagner A., Weber S., Hust M., ... , Frenzel A., Schirrmann T. (2013)

High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells

BMC Biotechnology, 13 (1)

DOI: 10.1186/1472-6750-13-52 HZI-Repositorium PubMed

de Groot J.C., Weidner C., Krausze J., Kawamoto K., Schroeder F.C., ... , Sauer S, Büssow K. (2013)

Structural Characterization of Amorfrutins Bound to the Peroxisome Proliferator-Activated Receptor gamma

Journal of Medicinal Chemistry, 56 (4)

DOI: 10.1021/jm3013272 HZI-Repositorium PubMed

Wilke S., Krausze J., Büssow Konrad (2012)

Crystal structure of the conserved domain of the DC lysosomal associated membrane protein: implications for the lysosomal glycocalyx

BMC Biology, 10

DOI: 10.1186/1741-7007-10-62 HZI-Repositorium PubMed

Weidner C., de Groot J.C., Prasad A., Freiwald A., Quedenau C., Kliem M., Witzke A., Kodelja V., Han C.T., Giegold S., Baumann M., Klebl B., Siems K., Muller-Kuhrt L., Schurmann A., Schuler R., Pfeiffer A.F., Schroeder F.C., ... , Büssow Konrad, Sauer S. (2012)

Amorfrutins are potent antidiabetic dietary natural products

Proceedings ot the National Academy of Siences USA, 109 (19)

Ergin A., Syrbe U., Scheer R., Thiel A., Adam T., Büssow Konrad*, Duchmann R., ... , Zeitz M., Sieper J. (2011)

Impaired Peripheral Th1 CD4+ T Cell Response to Escherichia coli Proteins in Patients with Crohn's Disease and Ankylosing Spondylitis3529

Journal of Clinial Immunology, 31 (6)

Wilke S., Gröbe Lothar*, Maffenbeier V., Jäger Volker*, Gossen M., Josewski J., Duda A., Polle L., Owens R.J., Wirth Dagmar*, Heinz Dirk* W., ... , van den Heuvel Joop*, Büssow Konrad* (2011)

Streamlining homogeneous glycoprotein production for biophysical and structural applications by targeted cell line development

PLoS ONE, 6 (12)

DOI: 10.1371/journal.pone.0027829 HZI-Repositorium PubMed

de Groot J.C., Schluter K., Carius Y., Quedenau C., Vingadassalom D., Faix J., Weiss S.M., Reichelt J., Standfuss-Gabisch C., Lesser C.F., Leong J.M., Heinz Dirk W.*, ... , Büssow Konrad*, Stradal Theresia E.* (2011)

Structural Basis for Complex Formation between Human IRSp53 and the Translocated Intimin Receptor Tir of Enterohemorrhagic E. coli

Structure, 19 (9)

DOI: 10.1016/j.str.2011.06.015 HZI-Repositorium PubMed

Busso D., Peleg Y., Heidebrecht T., Romier C., Jacobovitch Y., Dantes A., Salim L., Troesch E., Schuetz A., Heinemann U., Folkers G.E., Geerlof A., Wilmanns M., Polewacz A., Quedenau C., Bussow Konrad*, Adamson R., Blagova E., Walton J., Cartwright J.L., Bird L.E., Owens R.J., Berrow N.S., Wilson K.S., Sussman J.L., ... , Perrakis A., Celie P.H. (2011)

Expression of protein complexes using multiple Escherichia coli protein co-expression systems: A benchmarking study

Journal of Structural Biology, 175 (2)

DOI: 10.1016/j.jsb.2011.03.004 HZI-Repositorium PubMed

Wilke S., Krausze J., Gossen M., Gröbe Lothar*, Jager V., Gherardi E., ... , van den Heuvel J., Bussow Konrad* (2010)

Glycoprotein production for structure analysis with stable, glycosylation mutant CHO cell lines established by fluorescence-activated cell sorting

Protein Science, 19 (6)

Sievert V., Ergin A., Büssow Konrad* (2008)

High throughput cloning with restriction enzymes

Methods in Molecular Biology, 426

Schulze J.O., Quedenau C., Roske Y., Adam T., Schuler H., Behlke J., Turnbull A.P., Sievert V., Scheich C., Mueller U., ... , Heinemann U., Büssow Konrad* (2008)

Structural and functional characterization of human Iba proteins

FEBS Journal, 275 (18)

DOI: 10.1111/j.1742-4658.2008.06605.x HZI-Repositorium PubMed

Manjasetty B.A., Turnbull A.P., Panjikar S., Bussow Konrad*, Chance M.R. (2008)

Automated technologies and novel techniques to accelerate protein crystallography for structural genomics

Proteomics, 8 (4)

Scheich C., Kümmel D., Soumailakakis D., Heinemann U., Büssow K. (2007)

Vectors for co-expression of an unrestricted number of proteins

Nucleic Acids Research, 35

DOI: 10.1093/nar/gkm067 HZI-Repositorium PubMed

Ergin A., Büssow K., Sieper J., Thiel A., Duchmann R., Adam T. (2007)

Homologous high-throughput expression and purification of highly conserved E. coli