Prof. Dr. Dagmar Wirth

Prof. Dr. Dagmar Wirth
Forschungsgruppenleiterin

Dagmar Wirth studierte in Braunschweig Chemie. Bereits während ihrer Promotion an der GBF – dem heutigen HZI – wandte sie sich der Genregulation von Säugerzellen zu und beschäftigte sich mit chromosomalen Elementen, die Genexpression beeinflussen. Anschließend forschte sie als Postdoc an der Rekombination in Säugerzellen und Viren und entwickelte Viren für gentherapeutische Anwendungen.

Nach ihrer Tätigkeit als Wissenschaftlerin in der Abteilung für Klinische Immunologie kehrte sie im Jahr 2004  an die GBF zurück und wurde kurze Zeit später Projektleiterin für Zelluläre Modelle und Forschungsgruppenleiterin für Modellsysteme für Infektion und Immunität (MSYS). Für diese  Forschungsaktivitäten in MSYS entwickelt sie auch neue transgene Mausmodelle mit vorhersagbarer und kontrollierbarer Genexpression.

Um die Expertise zur Generierung transgener Modelle allen Forschungsgruppen des Zentrums zur Verfügung zu stellen und weiterzuentwickeln, wurde in 2012 die Serviceeinheit „Transgene Mäuse“ (TGSM) eingerichtet, die Dagmar Wirth leitet.

Publikationen
Gödecke N., Hauser H., Wirth D. (2024)
Stable Expression by Lentiviral Transduction of Cells
Methods Mol Biol, 2. edition (10)

Morales A.E., Gumenick R., Genovese C.M., Jang Y.Y., Ouedraogo A., Garayo M.I., Pannellini T., Patel S., Bott M.E., Alvarez J., Mun S.S., Totonchy J., Gautam A., La Mora J.D., Chang S., Wirth D., Horenstein M., Dao T., Scheinberg D.A., Rubinstein P.G., Semeere A., Martin J., Godfrey C.C., Moser C.B., Matining R.M., Campbell T.B., Borok M.Z., ... , Krown S.E., Cesarman E. (2024)
Wilms' tumor 1 (WT1) antigen is overexpressed in Kaposi Sarcoma and is regulated by KSHV vFLIP
PLoS Pathogens, 20 (1)

Kutle I., Dittrich A., Wirth D. (2023)
Mouse Models for Human Herpesviruses
Pathogens, 12 (7)

Gödecke N., Herrmann S., Weichelt V., Wirth D. (2023)
A Ubiquitous Chromatin Opening Element and DNA Demethylation Facilitate Doxycycline-Controlled Expression during Differentiation and in Transgenic Mice
ACS Synth Biol, 12 (2)

Latorre Y., Torres M., Vergara M., Berrios J., Sampayo M.M., Gödecke N., Wirth D., Hauser H., ... , Dickson A.J., Altamirano C. (2023)
Engineering of Chinese hamster ovary cells for co-overexpressing MYC and XBP1s increased cell proliferation and recombinant EPO production
Sci.Rep., 13 (1)

Schwerk J., Kemper L., Bussey K.A., Lienenklaus S., Weiss S., Cicin-Sain L., Kröger A., Kalinke U., Collins C.M., Speck S.H., Messerle M., Wirth D., Brinkmann M.M., ... , Hauser H., Köster M. (2022)
Type I Interferon Signaling Controls Gammaherpesvirus Latency In Vivo
Pathogens, 11 (12)

Lee M.J., Lee J., Kang S.K., Wirth D., Yoo S.M., ... , Park C., Lee M.S. (2022)
CXCL1 confers a survival advantage in Kaposi's sarcoma-associated herpesvirus-infected human endothelial cells through STAT3 phosphorylation
J.Med.Virol.

Gaur P., Riehn M., Zha L., Köster M., Hauser H., Wirth D. (2021)
Defective interferon amplification and impaired host responses against influenza virus in obese mice
Obesity.(Silver.Spring), 29 (8)

Rahman S.K., Ansari M.A., Gaur P., Ahmad I., Chakravarty C., Verma D.K., Sharma A., Chhibber S., Nehal N., ... , Wirth D., Lal S.K. (2021)
The Immunomodulatory CEA Cell Adhesion Molecule 6 (CEACAM6/CD66c) Is a Protein Receptor for the Influenza a Virus
Viruses., 13 (5)

Kumashie K.G., Cebula M., Hagedorn C., Kreppel F., Pils M.C., Koch-Nolte F., ... , Rissiek B., Wirth D. (2021)
Improved Functionality of Exhausted Intrahepatic CXCR5+ CD8+ T Cells Contributes to Chronic Antigen Clearance Upon Immunomodulation
Front Immunol., 11

Dubich T., Dittrich A., Bousset K., Geffers R., Büsche G., Köster M., Hauser H., ... , Schulz T.F., Wirth D. (2021)
3D culture conditions support Kaposi's sarcoma herpesvirus (KSHV) maintenance and viral spread in endothelial cells
J.Mol.Med.(Berl), 99 (3)

Gödecke N., Herrmann S., Hauser H., Mayer-Bartschmid A., Trautwein M., Wirth D. (2021)
Rational Design of Single Copy Expression Cassettes in Defined Chromosomal Sites Overcomes Intraclonal Cell-to-Cell Expression Heterogeneity and Ensures Robust Antibody Production
ACS Synth.Biol., 10 (1)

Gödecke N., Riedel J., Herrmann S., Behme S., Rand U., Kubsch T., Cicin-Sain L., Hauser H., ... , Köster M., Wirth D. (2020)
Synthetic rewiring and boosting type I interferon responses for visualization and counteracting viral infections
Nucleic Acids Res., 48 (20)

Brown R.J.P., Tegtmeyer B., Sheldon J., Khera T., Anggakusuma, Todt D., Vieyres G., Weller R., Joecks S., Zhang Y., Sake S., Bankwitz D., Welsch K., Ginkel C., Engelmann M., Gerold G., Steinmann E., Yuan Q., Ott M., Vondran F.W.R., Krey T., Ströh L.J., Miskey C., Ivics Z., Herder V., Baumgärtner W., Lauber C., Seifert M., Tarr A.W., Mcclure C.P., Randall G., Baktash Y., Ploss A., Thi V.L.D., Michailidis E., Saeed M., Verhoye L., Meuleman P., Goedecke N., Wirth D., ... , Rice C.M., Pietschmann T. (2020)
Liver-expressed Cd302 and Cr1l limit hepatitis C virus cross-species transmission to mice
Sci Adv, 6 (45)

Wahlicht T., Vieyres G., Bruns S.A., Meumann N., Büning H., Hauser H., Schmitz I., ... , Pietschmann T., Wirth D. (2020)
Controlled Functional Zonation of Hepatocytes in Vitro by Engineering of Wnt Signaling
ACS Synth.Biol., 9 (7)

Beauclair G., Naimo E., Dubich T., Rückert J., Koch S., Dhingra A., ... , Wirth D., Schulz T.F. (2020)
Targeting Kaposi's Sarcoma-Associated Herpesvirus ORF21 Tyrosine Kinase and Viral Lytic Reactivation by Tyrosine Kinase Inhibitors Approved for Clinical Use
J.Virol., 94 (5)

Aghajanian H., Kimura T., Rurik J.G., Hancock A.S., Leibowitz M.S., Li L., Scholler J., Monslow J., Lo A., Han W., Wang T., Bedi K., Morley M.P., Saldana R.A.L., Bolar N.A., McDaid K., Assenmacher C.A., Smith C.L., Wirth D., June C.H., Margulies K.B., ... , Jain R., Albelda S.M. (2019)
Author Correction: Targeting cardiac fibrosis with engineered T cells (Nature, (2019), 573, 7774, (430-433), 10.1038/s41586-019-1546-z)
Nature, 576 (7785)

Aghajanian H., Kimura T., Rurik J.G., Hancock A.S., Leibowitz M.S., Li L., Scholler J., Monslow J., Lo A., Han W., Wang T., Bedi K., Morley M.P., Linares Saldana R.A., Bolar N.A., McDaid K., Assenmacher C.A., Smith C.L., Wirth D., June C.H., Margulies K.B., Jain R., Puré E., ... , Albelda S.M., Epstein J.A. (2019)
Targeting cardiac fibrosis with engineered T cells
Nature, 573 (7774)

Laske T., Bachmann M., Dostert M., Karlas A., Wirth D., Frensing T., Meyer T.F., ... , Hauser H., Reichl U. (2019)
Model-based analysis of influenza A virus replication in genetically engineered cell lines elucidates the impact of host cell factors on key kinetic parameters of virus growth
PLoS Comput.Biol., 15 (4)

Dubich T., Lieske A., Santag S., Beauclair G., Rückert J., Herrmann J., Gorges J., Büsche G., Kazmaier U., Hauser H., Stadler M., ... , Schulz T.F., Wirth D. (2019)
An endothelial cell line infected by Kaposi's sarcoma-associated herpes virus (KSHV) allows the investigation of Kaposi's sarcoma and the validation of novel viral inhibitors in vitro and in vivo
Journal of Molecular Medicine, 97 (3)

Ullah S., Seidel K., Türkkan S., Warwas D.P., Dubich T., Rohde M., Hauser H., Behrens P., Kirschning A., ... , Köster M., Wirth D. (2019)
Macrophage entrapped silica coated superparamagnetic iron oxide particles for controlled drug release in a 3D cancer model
J.Control Release, 294

Becker J., Kinast V., Döring M., Lipps C., Duran V., Spanier J., Tegtmeyer P.K., Wirth D., Cicin-Sain L., ... , Alcami A., Kalinke U. (2018)
Human monocyte-derived macrophages inhibit HCMV spread independent of classical antiviral cytokines
Virulence., 9 (1)

Tolle C., Riedel J., Mikolai C., Winkel A., Stiesch M., ... , Wirth D., Menzel H. (2018)
Biocompatible Coatings from Smart Biopolymer Nanoparticles for Enzymatically Induced Drug Release
Biomolecules, 8 (4)

Gödecke N., Hauser H., Wirth D. (2018)
Stable Expression by Lentiviral Transduction of Cells
Methods Mol.Biol., 1850 (chapter 4)

Ziegler P.K., Bollrath J., Pallangyo C.K., Matsutani T., Canli Í., De Oliveira T., Diamanti M.A., Müller N., Gamrekelashvili J., Putoczki T., Horst D., Mankan A.K., Öner M.G., Müller S., Müller-Höcker J., Kirchner T., Slotta-Huspenina J., Taketo M.M., Reinheckel T., Dröse S., Larner A.C., Wels W.S., Ernst M., Greten T.F., Arkan M.C., Korn T., ... , Wirth D., Greten F.R. (2018)
Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis
Cell, 174 (1)

Sapich S., Hittinger M., Hendrix-Jastrzebski R., Repnik U., Griffiths G., May T., Wirth D., Bals R., ... , Schneider-Daum N., Lehr C.M. (2018)
Murine alveolar epithelial cells and their lentivirus-mediated immortalisation
Altern.Lab Anim, 46 (2)

Lipps C., Klein F., Wahlicht T., Seiffert V., Butueva M., Zauers J., Truschel T., Luckner M., Köster M., MacLeod R., Pezoldt J., Hühn J., Yuan Q., Muller P.P., Kempf H., Zweigerdt R., Dittrich-Breiholz O., Pufe T., Beckmann R., Drescher W., Riancho J., Sanudo C., Korff T., Opalka B., Rebmann V., Gothert J.R., Alves P.M., Ott M., Schucht R., Hauser H., ... , Wirth D., May T. (2018)
Expansion of functional personalized cells with specific transgene combinations
Nat.Commun., 9 (1)

Pieters T., Haenebalcke L., Bruneel K., Vandamme N., Hochepied T., Van Hengel J., Wirth D., Berx G., Haigh J.J., ... , van Roy F., Goossens S. (2017)
Structure-function studies in mouse embryonic stem cells using recombinase-mediated cassette exchange
J.Visualized Exp., 2017 (122)

Zhao G., Wirth D., Schmitz I., Meyer-Hermann M. (2017)
A mathematical model of the impact of insulin secretion dynamics on selective hepatic insulin resistance
Nat.Commun., 8 (1)

Selvakumar T.A., Bhushal S., Kalinke U., Wirth D., Hauser H., ... , Köster M., Hornef M.W. (2017)
Identification of a Predominantly Interferon-lambda-Induced Transcriptional Profile in Murine Intestinal Epithelial Cells
Front Immunol., 8

Riehn M., Cebula M., Hauser H., Wirth D. (2017)
CpG-ODN Facilitates Effective Intratracheal Immunization and Recall of Memory against Neoantigen-Expressing Alveolar Cells
Front Immunol., 8

Gödecke N., Zha L., Spencer S., Behme S., Riemer P., Rehli M., ... , Hauser H., Wirth D. (2017)
Controlled re-activation of epigenetically silenced Tet promoter-driven transgene expression by targeted demethylation
Nucleic Acids Res., 45 (16)

Pasztoi M., Pezoldt J., Beckstette M., Lipps C., Wirth D., Rohde M., Paloczi K., ... , Buzas E.I., Hühn J. (2017)
Mesenteric lymph node stromal cell-derived extracellular vesicles contribute to peripheral de novo induction of Foxp3+ regulatory T cells
Eur.J.Immunol., 47 (12)

Kunkiel J., Godecke N., Ackermann M., Hoffmann D., Schambach A., Lachmann N., ... , Wirth D., Moritz T. (2017)
The CpG-sites of the CBX3 ubiquitous chromatin opening element are critical structural determinants for the anti-silencing function
Sci.Rep., 7 (1)

Cebula M., Riehn M., Hillebrand U., Kratzer R.F., Kreppel F., Koutsoumpli G., Daemen T., ... , Hauser H., Wirth D. (2017)
TLR9-Mediated Conditioning of Liver Environment Is Essential for Successful Intrahepatic Immunotherapy and Effective Memory Recall
Mol.Ther.

Strandt H., Pinheiro D.F., Kaplan D.H., Wirth D., Gratz I.K., Hammerl P., ... , Thalhamer J., Stoecklinger A. (2017)
Neoantigen Expression in Steady-State Langerhans Cells Induces CTL Tolerance
J.Immunol., 199 (5)

Bhushal S., Wolfsmüller M., Selvakumar T.A., Kemper L., Wirth D., Hornef M.W., ... , Hauser H., Köster M. (2017)
Cell polarization and epigenetic status shape the heterogeneous response to type III interferons in intestinal epithelial cells
Front.Immunol., 8 (JUN)

Schmidt M., Laufs S., Aiuti A., Aubourg P., Baum C., Biasco L., Cartier N., Hauser H., Montini E., Moullier P., Snyder R.O., ... , Wirth D., von Kalle C. (2012)
Biosafety analysis in preclinical and clinical studies
(B2-3)

Gama-Norton L., Hauser H., Wirth D. (2012)
Restrictions and requirements for stable lentiviral vector production in HEK293 cells
(A3-6)

Coroadinha A.S., Wirth D., Rodrigues A.F., Gama-Norton L., Duros C., Artus A., Cohen-Haguenauer O., Marques Alves P., Cruz P.E., ... , Carrondo M.J.T., Hauser H. (2012)
Modular retroviral producer cell lines
(A2-4)

Gaur P., Cebula M., Riehn M., Hillebrand U., Hauser H., Wirth D. (2017)
Diet induced obesity has an influence on intrahepatic T cell responses
Metab.Clin.Exp., 69

Lipps C., Badar M., Butueva M., Dubich T., Singh V.V., Rau S., Weber A., Kracht M., Koster M., May T., Schulz T.F., ... , Hauser H., Wirth D. (2016)
Proliferation status defines functional properties of endothelial cells
Cell Mol.Life Sci, 74 (7)

Pieters T., Goossens S., Haenebalcke L., Andries V., Stryjewska A., De Rycke R., Lemeire K., Hochepied T., Huylebroeck D., Berx G., Stemmler M.P., Wirth D., Haigh J.J., ... , van Hengel J., van Roy F. (2016)
p120 Catenin-Mediated Stabilization of E-Cadherin Is Essential for Primitive Endoderm Specification
PLoS Genet., 12 (8)

Spencer S., Gugliotta A., Gödecke N., Hauser H., Wirth D. (2016)
Epigenetic modulations rendering cell-to-cell variability and phenotypic metastability
J Genet.Genomics, 43

Lipps C., May T., Wirth D. (2014)
Cell lines: Generation of cell lines and biotechnological applications

Köster M., Wirth D. (2016)
Zellen im Zeitraffer - Dynamik der Genexpression in Echtzeit [Cells in time lapse - Dynamics of gene expression in real time]
Biospektrum, 22 (1)

Rais B., Köster M., Rahim M.I., Pils M., Seitz J.M., Hauser H., ... , Wirth D., Müller P.P. (2016)
Evaluation of the inflammatory potential of implant materials in a mouse model by bioluminescent imaging of intravenously injected bone marrow cells
J Biomed.Mater.Res A, 104 (9)

Lipps C., May T., Wirth D. (2014)
Generation of cell lines and biotechnological applications
(2.1)

Kruse N., Spencer S., Wirth D. (2014)
Rational Approaches for Transgene Expression: Targeted Integration and Episomal Maintenance

Kuehn A., Kletting S., de Souza Carvalho-Wodarz, Repnik U., Griffiths G., Fischer U., Meese E., Huwer H., Wirth D., May T., ... , Schneider-Daum N., Lehr C.M. (2016)
Human alveolar epithelial cells expressing tight junctions to model the air-blood barrier
ALTEX., 33

Lempp F.A., Mutz P., Lipps C., Wirth D., Bartenschlager R., Urban S. (2015)
Evidence that hepatitis B virus replication in mouse cells is limited by the lack of a host cell dependency factor
J Hepatol., 64

Bachmann M., Breitwieser T., Lipps C., Wirth D., Jordan I., ... , Reichl U., Frensing T. (2016)
Impaired antiviral response of adenovirus-transformed cell lines supports virus replication
J.Gen.Virol., 97 (2)

Tran D.D.H., Koch A., Saran S., Armbrecht M., Ewald F., Koch M., Wahlicht T., Wirth D., Braun A., Nashan B., ... , Gaestel M., Tamura T. (2016)
Extracellular-signal regulated kinase (Erk1/2), mitogen-activated protein kinase-activated protein kinase 2 (MK2) and tristetraprolin (TTP) comprehensively regulate injury-induced immediate early gene (IEG) response in in vitro liver organ culture
Cell.Signal., 28 (5)

Ochel A., Cebula M., Riehn M., Hillebrand U., Lipps C., Schirmbeck R., ... , Hauser H., Wirth D. (2016)
Effective intrahepatic CD8+ T-cell immune responses are induced by low but not high numbers of antigen-expressing hepatocytes
Cell Mol.Immunol., 13

Lieber D., Hochdorfer D., Stoehr D., Schubert A., Lotfi R., May T., ... , Wirth D., Sinzger C. (2015)
A permanently growing human endothelial cell line supports productive infection with human cytomegalovirus under conditional cell growth arrest
Biotechniques, 59 (3)

Rand U., Riedel J., Hillebrand U., Shin D., Willenberg S., Behme S., Klawonn F., Köster M., ... , Hauser H., Wirth D. (2015)
Single-cell analysis reveals heterogeneity in onset of transgene expression from synthetic tetracycline-dependent promoters
Biotechnol.J., 10 (2)

Spencer S., Gugliotta A., Koenitzer J., Hauser Hansjörg, Wirth Dagmar (2014)
Stability of single copy transgene expression in CHOK1 cells is affected by histone modifications but not by DNA methylation
Journal of Biotechnology, 195C

Rand U., Hillebrand Upneet, Sievers S., Willenberg S., Köster Mario, ... , Hauser Hansjörg, Wirth Dagmar (2014)
Uncoupling of the dynamics of host-pathogen interaction uncovers new mechanisms of viral interferon antagonism at the single-cell level
Nucleic Acids Res, 42 (13)

Dag F., Dolken L., Holzki J., Drabig A., Weingartner A., Schwerk J., Lienenklaus S., Conte I., Geffers R., Davenport C., Rand U., Köster M., Weiss S., Adler B., Wirth D., Messerle M., ... , Hauser H., Cicin-Sain L. (2014)
Reversible silencing of cytomegalovirus genomes by type I interferon governs virus latency
PLoS Pathogens, 10 (2)

Botezatu L., Sievers S., Gama-Norton L., Schucht Roland, Hauser Hansjörg, Wirth Dagmar (2012)
Genetic aspects of cell line development from a synthetic biology perspective
Advances in Biochemical Engineering/Biotechnology, 127

Dag F., Weingartner A., Butueva M., Conte I., Holzki J., May T., Adler B., ... , Wirth D., In-Ain I. (2013)
A new reporter mouse cytomegalovirus reveals maintained immediate-early gene expression but poor virus replication in cycling liver sinusoidal endothelial cells
Virology Journal, 10 (1)

Lipps C., May T., Hauser H., Wirth D. (2013)
Eternity and functionality - rational access to physiologically relevant cell lines
Biological Chemistry, 394 (12)

Cebula Marcin, Ochel Aaron, Hillebrand Upneet, Pils Marina C., Schirmbeck R., ... , Hauser Hansjörg, Wirth Dagmar (2013)
An Inducible Transgenic Mouse Model for Immune Mediated Hepatitis Showing Clearance of Antigen Expressing Hepatocytes by CD8+ T Cells
PLoS ONE, 8 (7)

Haenebalcke L., Goossens S., Naessens M., Kruse N., Ghahremani M.F., Bartunkova S., Haigh K., Pieters T., Dierickx P., Drogat B., Nyabi O., ... , Wirth Dagmar, Haigh J.J. (2013)
Efficient ROSA26-Based Conditional and/or Inducible Transgenesis Using RMCE-Compatible F1 Hybrid Mouse Embryonic Stem Cells
Stem Cell Reviews and Reports, 9

Haenebalcke L., Goossens S., Dierickx P., Bartunkova S., D'Hont J., Haigh K., Hochepied T., Wirth Dagmar, ... , Nagy A., Haigh J.J. (2013)
The ROSA26-iPSC mouse: a conditional, inducible, and exchangeable resource for studying cellular (De)differentiation
Cell Reports, 3 (2)

May T., Hauser Hansjörg, Wirth Dagmar (2012)
Cell systems: Functional cell lines through new immortalization protocols [Zellsysteme: Funktionale zelllinien durch neue immortalisierungsprotokolle]
Biospektrum, 18 (4)

Nehlsen K., da Gama-Norton L., Schucht R., Hauser Hansjörg*, Wirth Dagmar* (2011)
Towards rational engineering of cells: Recombinant gene expression in defined chromosomal loci
BMC proceedings, 5 ((Suppl 8))

Cruz P.E., Rodrigues T., Carmo M., Wirth Dagmar*, Amaral A.I., ... , Alves P.M., Coroadinha A.S. (2011)
Manufacturing of retroviruses
Methods in Molecular Biology, 737

Wilke S., Gröbe Lothar*, Maffenbeier V., Jäger Volker*, Gossen M., Josewski J., Duda A., Polle L., Owens R.J., Wirth Dagmar*, Heinz Dirk* W., ... , van den Heuvel Joop*, Büssow Konrad* (2011)
Streamlining homogeneous glycoprotein production for biophysical and structural applications by targeted cell line development
PLoS ONE, 6 (12)

Gama-Norton L., Botezatu L., Herrmann S., Schweizer M., Alves P.M., ... , Hauser Hansjörg*, Wirth Dagmar* (2011)
Lentivirus production is influenced by SV40 large T-antigen and chromosomal integration of the vector in HEK293 cells
Human gene therapy, 22 (10)

Alkharsah K.R., Singh V.V., Bosco R., Santag S., Grundhoff A., Konrad A., Sturzl M., Wirth Dagmar*, Dittrich-Breiholz O., ... , Kracht M., Schulz T.F. (2011)
Deletion of Kaposi's sarcoma-associated herpesvirus FLICE inhibitory protein, vFLIP, from the viral genome compromises the activation of STAT1-responsive cellular genes and spindle cell formation in endothelial cells
Journal of Virology, 85 (19)

Schucht R., Lydford S., Andzinski L., Zauers J., Cooper J., Hauser Hansjörg*, ... , Wirth Dagmar*, May T. (2011)
Rapid establishment of G-protein-coupled receptor-expressing cell lines by site-specific integration
Journal of Biomolecular Screening, 16 (3)

Sandhu U., Cebula M., Behme S., Riemer P., Wodarczyk C., Metzger D., Reimann J., Schirmbeck R., ... , Hauser Hansjörg*, Wirth Dagmar* (2011)
Strict control of transgene expression in a mouse model for sensitive biological applications based on RMCE compatible ES cells
Nucleic Acids Research, 39 (1)

Carrondo M., Panet M., Wirth Dagmar*, Coroadinha A.S., Cruz P., Falk H., Schucht R., Dupont F., Geny-Fiamma C., ... , Merten O.W., Hauser Hansjörg* (2011)
Integrated strategy for the production of therapeutic retroviral vectors
Human gene therapy, 22 (3)

Schucht R., Wirth Dagmar*, May T. (2010)
Precise regulation of transgene expression level and control of cell physiology
Cell Biology and Toxicology, 26 (1)

Loew R., Meyer Y., Kuehlcke K., Gama-Norton L., Wirth D., Hauser H., Stein S., Grez M., Thornhill S., Thrasher A., ... , Baum C., Schambach A. (2010)
A new PG13-based packaging cell line for stable production of clinical-grade self-inactivating gamma-retroviral vectors using targeted integration
Gene Therapy, 17 (2)

May T., Butueva M., Bantner S., Markusic D., Seppen J., MacLeod R.A., Weich H., ... , Hauser Hansjörg*, Wirth Dagmar* (2010)
Synthetic gene regulation circuits for control of cell expansion
Tissue Engineering - Part A, 16 (2)

Gama-Norton L., Herrmann S., Schucht R., Coroadinha A.S., Low R., Alves P.M., Bartholomae C.C., Schmidt M., Baum C., Schambach A., ... , Hauser Hansjörg*, Wirth Dagmar* (2010)
Retroviral vector performance in defined chromosomal Loci of modular packaging cell lines
Human gene therapy, 21 (8)

Wirth Dagmar*, Gama-Norton L., Schucht R., Nehlsen K., Hauser Hansjörg* (2009)
Site-directed engineering of defined chromosomal sites for recombinant protein and virus expression
BioPharm International, 22 (7)

Nehlsen K., Herrmann S., Zauers J, Hauser Hansjörg*, Wirth Dagmar* (2010)
Toxin-antitoxin based transgene expression in mammalian cells.
Nucleic Acids Research, 38 (5)

Nehlsen K., Schucht R., da Gama-Norton L., Kromer W., Baer A., Cayli A., ... , Hauser Hansjörg*, Wirth Dagmar* (2009)
Recombinant protein expression by targeting pre-selected chromosomal loci
BMC Biotechnology, 14 (9)

May T., Hauser Hansjörg*, Wirth Dagmar* (2007)
In vitro expansion of tissue cells by conditional proliferation
Methods in Molecular Medicine, 140

May T., Eccleston L., Herrmann S., Hauser Hansjörg*, Goncalves J., Wirth Dagmar* (2008)
Bimodal and hysteretic expression in mammalian cells from a synthetic gene circuit
PLoS ONE, 3 (6)

Wirth Dagmar*, Gama-Norton L., Riemer P., Sandhu U., Schucht R., Hauser Hans-Jörg* (2007)
Road to precision: recombinase-based targeting technologies for genome engineering
Current Opinion in Biotechnology, 18 (5)

May Tobias*, Hauser Hans-Jörg*, Wirth Dagmar* (2007)
In vitro expansion of tissue cells by conditional proliferation

Kues W.A., Schwinzer R., Wirth Dagmar*, Verhoeyen E., Lemme E., Herrmann D., Barg-Kues B., Hauser Hansjörg*, ... , Wonigeit K., Niemann H. (2006)
Reactivation of silenced tetracycline-controlled hRCA constructs in transgenic pigs
Xenotransplantation, 13 (6)

May T., Hauser Hansjörg*, Wirth Dagmar* (2006)
Current status of transcriptional regulation systems
Cytotechnology, 50

Kues W.A., Schwinzer R., Wirth Dagmar*, Verhoeyen E., Lemme E., Herrmann D., Barg-Kues B., Hauser Hansjörg*, ... , Wonigkeit K., Niemann H. (2006)
Epigenetic silencing and tissue independent expression of a novel tetracycline inducible system in double transgenic pigs
FASEB journal, 20

Coroadinha A.S., Schucht R., Gama-Norton L., Wirth Dagmar*, Hauser Hansjörg*, Carrondo M.J.T. (2006)
The use of recombinase cassette exchange in retroviral vector producer cell lines: predictability and efficiency in transgene replacement
Journal of Biotechnology, 124 (2)

Deppenmeier S., Bock O., Mengel M., Niemann H., Kues W., Lemme E., Wirth Dagmar*, ... , Wonigeit K., Kreipe H. (2006)
Health status of transgenic pigs expressing the human complement regulatory protein CD59
Xenotransplantation, 13 (4)

Schucht R., Coroadinha A.S., Zanta-Boussif M.A., Verhoeyen E., Carrondo M.J.T., ... , Hauser Hansjörg*, Wirth Dagmar* (2006)
A new generation of retroviral producer cells: Predictable and stable virus production by Flp mediated site-specific integration of retroviral vectors
Molecular therapy, 14

Thiefes A., Wolter S., Mushinski J.S., Hoffmann E., Dittrich-Breiholz O., Graue N., Dörrie A., Schneider H., Wirth D., Luckow B., ... , Resch K., Kracht M. (2005)
Simultaneous blockade of NFkappaB, JNK and p38 MAPK by a kinase-inactive mutant of the protein kinase TAK1 sensitizes cells to apoptosis and affects a distinct spectrum of TNF target genes
Journal of Biological Chemistry, 280

May T., Müller P., Weich H., Froese N., Deutsch U., Wirth D., ... , Kröger A., Hauser H. (2005)
Establishment of murine cell lines by constitutive and conditional immortalization
Journal of Biotechnology, 120 (1)

May T., Wirth D., Hauser H., Müller P.P. (2005)
Transcriptionally regulated immortalization overcomes side effects of tmeperature-sensitive SV40 large T antigen
Biochemical and Biophysical Research Communications, 327 (3)

May T., Lindenmaier W., Wirth D., Müller P.P. (2004)
Application of a reversible immortalisation system for the generation of proliferation-controlled cell lines
Cytotechnology, 46

Weissenbach M., Clahsen T., Weber C., Spitzer D., Wirth D., Vestwever D., ... , Heinrich P.C., Schaper F. (2004)
Interleukin-6 is a direct mediator of T cell migration
European Journal of Immunology, 34

Unsinger J., Lindenmaier W., May T., Hauser H., Wirth D. (2004)
Stable and strictly controlled expression of LTR-flanked autoregulated expression cassettes upon adenoviral transfer
Biochemical and Biophysical Research Communications, 319

May T., Hauser H., Wirth D. (2004)
Transcriptional control of SV40 expression allows a complete reversion of immortalization
Nucleic Acids Research, 32

Wirth Dagmar, Hauser Hansjörg (2004)
Flp-mediated integration of expression cassettes into FRT tagged chromosomal loci
(31)

Müller P.P., Wirth D., Unsinger U., Hauser H. (2003)
Genetic approaches to recombinant protein production in mammalian cells

Spitzer D., Dittmar K.E., Rohde M., Hauser H., Wirth D. (2003)
Green fluorescent protein-tagged retroviral envelope protein for analysis of virus-cell interactions.
Journal of Virology, 77

Hirschmann F., Verhoeyen E., Wirth D., Bauwens S., Hauser H., Rudert M. (2002)
Vital marking of articular chondrocytes by retroviral infection using green fluorescence protein
Osteoarthritis and Cartilage, 10 (2)

Verhoeyen E., Hauser H., Wirth D. (2001)
Evaluation of retroviral vector design in defined chromosomal loci by Flp-mediated cassette replacement
Human gene therapy, 12 (8)

Unsinger J., Kroger A., Hauser H., Wirth D. (2001)
Retroviral vectors for the transduction of autoregulated, bidirectional expression cassettes
Molecular therapy, 4 (5)

Niemann H., Verhoeyen E., Wonigeit K., Lorenz R., Hecker J., Schwinzer R., Hauser H., Kues W.A., Halter R., Lemme E., Herrmann D., Winkler M., ... , Wirth D., Paul D. (2001)
Cytomegalovirus early promoter induced expression of hCD59 in porcine organs provides protection against hyperacute rejection
Transplantation, 72 (12)

Müller P., Oumard A., Wirth D., Kröger A., Hauser H. (2001)
Polyvalent vectors for coexpression of multiple genes

Hauser H., Spitzer D., Verhoeyen E., Unsinger J., Wirth D. (2000)
New approaches towards ex vivo and in vivo gene therapy
Cells Tissues Organs, 167 (2-3)