Special Seminar: "Gastric organoids as a 3D model to study chemotaxis and virulence of Helicobacter pylori"
3D model systems of mammalian cell lines have been recently developed, enabling scientists to study cellular processes as well as bacteria/host-cell interactions at a new level of detail. Compared to 2D cell-culture systems, 3D systems mimic tissue-like structures more effectively, are able to exhibit differential cellular functions and are, therefore, more predictive for in vivo responses. Gastric and intestinal organoids are the state-of-the art 3D system to study the gastrointestinal tract by generating intestine and stomach like structures within a simple tissue culture plate. In this talk I will discuss the gastric organoid model to study interactions between the bacterium Helicobacter pylori (a pathogen causing gastritis, gastric ulcers and gastric cancer) and gastric epithelial host cells.
Gastric organoids are generated by proliferation of adult stem cells derived from mammalian gastric glands and differentiate into the same distinct cell types as found inside these gastric glands including mucus cells (mucus secretion), parietal cells (acid producing), chief cells (pepsinogen secretion) and endocrine cells (hormone secretion). I will discuss how we use this model system in our lab, to understand how bacterial processes such as motility and chemotaxis enable the bacterium to target specific cell types in its natural environment, how bacteria sense their target cells and how this interaction leads to the induction of apoptosis in the host cells.
Gebäude und Raum
University of California Santa Cruz
1156 High Street
Santa Cruz, California USA