Prof. Dr. Henning Urlaub: “Identification of protein-RNA contact sites using mass spectrometry”

Prof. Urlaub

  • Überblick

    The investigation of protein–RNA interactions by various bioanalytical methods is receiving increasing attention. Many methods focus on the identification of direct protein–RNA contact sites after their fixation by UV induced cross-linking. While methods concentrating on the RNA sequence benefit greatly from advances in sequencing technology and yield valuable information on contact sites at single nucleotide resolution, there has been a lack of complementary methods on the protein level. Quantitative proteomics has been successfully applied to the identification of RNA-binding proteins per se, but so far has not allowed for resolving the protein sequence regions or even amino acids directly involved in RNA-binding. We present here a novel mass spectrometry-based experimental and data analysis workflow that enables the investigation of protein–RNA interactions on the amino acid and nucleotide levels, starting from complex biological samples after cross-linking in vitro and in vivo.


    Datum: 27.02.2014, 11:00

    Veranstaltungsort

    Helmholtz-Zentrum für Infektionsforschung Braunschweig

    Gebäude und Raum

    Forum, X 0.13a

    Referent

    Prof. Dr. Henning Urlaub

    Max-Planck-Institute for Biophysical Chemistry, Göttingen                         Bioanalytical Mass Spectrometry Group
    University Medical Center Göttingen, Bioanalytics                        
    Department of Clinical Chemistry

    Gastgeber

    Prof. Dr. Emmanuelle Charpentier