Minicircle DNA - non-viral vectors supporting viral vector production

Martin Schleef

  • Überblick

    It has been shown that by so-called reverse packaging backbone sequences of the ITR containing vector plasmid may be encapsidated during the generation of viral particles. As a result of such events, an AAV mediated transfer of the antibiotics resistance gene instead of the therapeutic gene into the target cells appears to be a potential risk of plasmid derived AAV vectors. This can be overcome by using minicircle DNA for e.g. AAV production.

    An overview on the general structure of minicircle systems, first results on manufacturing of AAV Helper & Packaging minicircles and transfer minicircles, as well as their use in AAV production are presented.

    Datum: 04.11.2014, 14:30


    Helmholtz-Zentrum für Infektionsforschung

    Gebäude und Raum

    Forum X1.04


    Dr. Martin Schleef, 

    PlasmidFactory GmbH & Co. KG


  • Sylvia Richter

    Sylvia Richter

    Abteilungssekretariat Genregulation und Differenzierung

    0531 6181-5001

    0531 6181-5002