In vitro Evolution of Small Molecules
In vitro evolution of biological molecules from random combinatorial libraries has started with peptides and protein by phage display technology in the mid 1980ies and was transferred to nucleic acids by SELEX. However, only recently small synthetic molecules became accessible to in vitro evolution. So-called DNA encoded chemical libraries (DELs) work by either recording or instructing the combinatorial synthesis of covalently attached compounds using DNA labels. Our new approach to DEL technology will be presented in comparison to other published examples. Every single compound can be easily identified by PCR amplification and sequencing. The combination with next generation sequencing technology allows an instant identification of specific binding molecules from large libraries in a single selection round. So far several chemical syntheses have been shown to be compatible with DNA, offering a promising new way of obtaining highly affine and specific compounds for various applications.
Web page of Jörn Glökler’s group:
HZI Forum X1.01
Dr. Jörn Glökler, group leader at the MPI for Molecular Genetics Berlin
Dr. Konrad Büssow