Flavin Dependent Enzymes in Natural Product Biosynthesis
Flavin cofactors play a critical role in a remarkable variety of enzyme catalyzed reactions. This versatility has gained further prominence in recent years with the discovery of new flavin dependent enzymes in natural product pathways. Two recent examples include halogenases and the biosynthesis of indolocarbazoles.
Current work by our laboratory in these two areas will be presented.
Groom K, Bhattacharya A, Zechel DL: Rebeccamycin and staurosporine biosynthesis: Insight into the mechanisms of the flavin-dependent monooxygenases RebC and StaC. ChemBioChem 2011, 12, 396-400.
Podzelinska K, Latimer R, Bhattacharya A, Vining LC, Zechel DL, Jia Z: The structure of CmlS, a flavindependent halogenase involved in the biosynthesis of chloramphenicol. J. Mol. Biol. 2010, 397, 316-331.
Professor Dr. David Zechel
Department of Chemistry, Queen's University, Kingston, CAN
Birthplace: Winnipeg, Manitoba, Canada, 1973
2010 - 2011 Sabbatical stay with Professor Andreas Bechthold, Institut für Pharmazeutische Wissenschaften, Albert-Ludwigs-Universität, Freiburg, Germany. Identification and genetic manipulation of natural product biosynthetic pathways in Streptomyces sp.
2010 - present Associate professor, Department of Chemistry, Queen’s University, Kingston, Ontario, Canada
2004 - 2010 Assistant professor, Department of Chemistry, Queen’s University
2002 - 2004 Post-doctoral fellow with Prof. Andreas Plückthun, Biochemisches Institut, Universität Zürich, Switzerland. Development of ribosome display for evolution of proteins. Selection of novel protein folds from random secondary-structure protein libraries.
2001 - 2002 Post-doctoral fellow with Prof. Gideon Davies, York Structural Biology Laboratory, University of York, UK. Study of glycosidase mechanism through X-ray crystallography, transition state analogue inhibitors, isothermal titration calorimetry and kinetic analysis.
1996 - 2001 Ph.D. student with Prof. Stephen Withers, Department of Chemistry, University of British Columbia, Canada. Study of glycosidase mechanism. Engineering mutant glycosidases (glycosynthases) for the synthesis of oligosaccharides and carbon fluorine bonds. Development of ESI-MS for the analysis of enzyme kinetics.
Awards and Fellowships
2007 Early Researcher Award
2006 Synlett and Synthesis Journal Award
2004 Boehringer Ingelheim Award for Organic or Bioorganic Chemistry (Ph.D thesis)
HIPS, Blg B2.1, Hörsaal 02
Prof. Dr. David Zechel
Director of HIPS, Prof. Dr. Rolf Müller