Recombinant Protein Expression

Proteins play an important role in infectious diseases. They not only take over central functions in the invasion and replication of pathogens, but also in the defensive reaction of our body or as a drug. For a detailed investigation of proteins, scientists often require them in large amounts and extremely pure state. Read more about how proteins are artificially produced and purified.

Publications

  • Baser,B.; Spehr,J.; Bussow,K.; van den,Heuvel J.; (2016). A method for specifically targeting two independent genomic integration sites for co-expression of genes in CHO cells. Methods: 95, 3-12 PubMed
  • Bleckmann,M.; Schmelz,S.; Schinkowski,C.; Scrima,A.; van den,Heuvel J.; (2016). Fast plasmid based protein expression analysis in insect cells using an automated SplitGFP screen. DOI: 10.1002/bit.25956 HZI repository PubMed
  • Bleckmann,M.; Schurig,M.; Chen,F.F.; Yen,Z.Z.; Lindemann,N.; Meyer,S.; Spehr,J.; van den,Heuvel J.; (2016). Identification of Essential Genetic Baculoviral Elements for Recombinant Protein Expression by Transactivation in Sf21 Insect Cells. PLoS ONE: 11 3, e0149424 DOI: 10.1371/journal.pone.0149424 HZI repository PubMed
  • Böldicke,T.; (2016). Preface., v-vi ISBN: 9783319224732 (ISBN); 9783319224725 (ISBN) DOI: Editorial WEBURLS
  • Marschall,A.L.J.; Dübel,S.; Böldicke,T.; (2016). Recent advances with ER targeted intrabodies.: Protein Targeting Compounds: Prediction, Selection and Activity of Specific Inhibitors. Springer International Publishing, 77-93 ISBN: 9783319224732 (ISBN); 9783319224725 (ISBN) DOI: Book WEBURLS
  • Zahid,M.; Lünsdorf,H.; Rinas,U.; (2015). Assessing stability and assembly of the hepatitis B surface antigen into virus-like particles during down-stream processing. Vaccine: DOI: 10.1016/j.vaccine.2015.05.066 HZI repository PubMed
  • Quade,N.; Boehringer,D.; Leibundgut,M.; van den,Heuvel J.; Ban,N.; (2015). Cryo-EM structure of Hepatitis C virus IRES bound to the human ribosome at 3.9-A resolution. Nature Communications: 6, 7646 DOI: 10.1038/ncomms8646 HZI repository PubMed
  • Bleckmann,M.; Fritz,M.H.; Bhuju,S.; Jarek,M.; Schurig,M.; Geffers,R.; Benes,V.; Besir,H.; van den,Heuvel J.; (2015). Genomic Analysis and Isolation of RNA Polymerase II Dependent Promoters from Spodoptera frugiperda. PLoS ONE: 10 8, e0132898 PubMed
  • Marschall,A.L.J.; Dübel,S.; Böldicke,T.; (2015). Specific in vivo knockdown of protein function by intrabodies. MAbs: 7 6, 1010-103519420862 DOI: Review WEBURLS
  • Büssow,K.; (2015). Stable mammalian producer cell lines for structural biology. 32, 81-900959440X DOI: 10.1016/j.sbi.2015.03.002 HZI repository PubMed
  • Kleinboelting,S.; Diaz,A.; Moniot,S.; van den Heuvel,Joop; Weyand,M.; Levin,L.R.; Buck,J.; Steegborn,C.; (2014). Crystal structures of human soluble adenylyl cyclase reveal mechanisms of catalysis and of its activation through bicarbonate. Proceedings of the National Academy of Sciences of the United States of America: 111 10, 3727-373210916490 WEBURLS
  • Kleinboelting,S.; van den,Heuvel Joop; Kambach,C.; Weyand,M.; Leipelt,M.; Steegborn,C.; (2014). Expression, purification, crystallization and preliminary X-ray diffraction analysis of a mammalian type 10 adenylyl cyclase. Acta Crystallographica Section F: Structural Biology and Crystallization Communications: 70 Pt 4, 467-469 DOI: 10.1107/S2053230X14004014 HZI repository PubMed
  • Marschall,A.L.J.; Single,F.N.; Schlarmann,K.; Bosio,A.; Strebe,N.; van den Heuvel,J.; Frenzel,A.; Dübel,S.; (2014). Functional knock down of VCAM1 in mice mediated by endoplasmatic reticulum retained intrabodies. MAbs: 6 6, 1394-140119420862 WEBURLS
  • Seras-Franzoso,J.; Peebo,K.; Garcia-Fruitos,E.; Vazquez,E.; Rinas,Ursula; Villaverde,A.; (2014). Improving protein delivery of fibroblast growth factor-2 from bacterial inclusion bodies used as cell culture substrates. Acta Biomaterialia: 10 3, 1354-135917427061 WEBURLS
  • Li,Z.; Carstensen,B.; Rinas,U.; (2014). Smart sustainable bottle (SSB) system for E. coli based recombinant protein production. Microbial Cell Factories: 13, 153 PubMed
  • Kleinboelting,S.; van den Heuvel,J.; Steegborn,C.; (2014). Structural analysis of human soluble adenylyl cyclase and crystal structures of its nucleotide complexes - Implications for cyclase catalysis and evolution. 281 18, 4151-41641742464X WEBURLS
  • Kleinboelting,S.; van den Heuvel,Joop; Steegborn,C.; (2014). Structural analysis of human soluble adenylyl cyclase and crystal structures of its nucleotide complexes - Implications for cyclase catalysis and evolution. FEBS Journal: 281 18, 4151-41641742464X WEBURLS
  • Li,Z.; Nimtz,Manfred; Rinas,Ursula; (2014). The metabolic potential of Escherichia coli BL21 in defined and rich medium. Microbial Cell Factories: 13 1, 45 DOI: 10.1186/1475-2859-13-45 HZI repository PubMed
  • Hofmeyer,T.; Schmelz,S.; Degiacomi,M.T.; Dal Peraro,M.; Daneschdar,M.; Scrima,Andrea; van den Heuvel,Joop; Heinz,Dirk W.; Kolmar,H.; (2013). Arranged sevenfold: Structural insights into the C-terminal oligomerization domain of human C4b-binding protein. Journal of Molecular Biology: 425 8, 1302-131700222836 DOI: 10.1016/j.jmb.2012.12.017 HZI repository PubMed
  • Ringel,P.; Krausze,Joern; van den Heuvel,Joop; Curth,U.; Pierik,A.J.; Herzog,S.; Mendel,R.R.; Kruse,T.; (2013). Biochemical characterization of molybdenum cofactor-free nitrate reductase from Neurospora crassa. Journal of Biological Chemistry: 288 20, 14657-1467100219258 WEBURLS
  • Khetarpal,N.; Poddar,A.; Nemani,S.K.; Dhar,N.; Patil,A.; Negi,P.; Perween,A.; Viswanathan,R.; Lünsdorf,Heinrich; Tyagi,P.; Raut,R.; Arora,U.; Jain,S.K.; Rinas,Ursula; Swaminathan,S.; Khanna,N.; (2013). Dengue-specific subviral nanoparticles: Design, creation and characterization. Journal of Nanobiotechnology: 11 1, 1514773155 DOI: 10.1186/1477-3155-11-15 HZI repository PubMed
  • Bice,I.; Celik,H.; Wolff,C.; Beutel,S.; Zahid,M.; Hitzmann,B.; Rinas,Ursula; Kasper,C.; Gerardy-Schahn,R.; Scheper,T.; (2013). Downstream processing of high chain length polysialic acid using membrane adsorbers and clay minerals for application in tissue engineering. Engineering in Life Sciences: 13 2, 140-14816180240 WEBURLS
  • Vallejo,L.F.; Rinas,Ursula; (2013). Folding and dimerization kinetics of bone morphogenetic protein-2, a member of the transforming growth factor-beta family. FEBS Journal: 280 1, 83-92 DOI: 10.1111/febs.12051 HZI repository PubMed
  • Jäger,Volker; Büssow,Konrad; Wagner,A.; Weber,S.; Hust,M.; Frenzel,A.; Schirrmann,T.; (2013). High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells. BMC Biotechnology: 13 1, 5214726750 WEBURLS
  • Kaleta,C.; Schauble,S.; Rinas,Ursula; Schuster,S.; (2013). Metabolic costs of amino acid and protein production in Escherichia coli. Biotechnology Journal: 8 9, 1105-1114 PubMed
  • Meyer,S.; Lorenz,C.; Baser,B.; Wordehoff,M.; Jäger,Volker; van den Heuvel,Joop; (2013). Multi-host expression system for recombinant production of challenging proteins. PLoS ONE: 8 7, e68674 DOI: 10.1371/journal.pone.0068674 HZI repository PubMed
  • Böldicke,Thomas; (2013). Polyclonal antibodies for detection of witches' broom disease of lime. Iranian Journal of Biotechnology: 11 3, 14417283043 WEBURLS
  • Gurramkonda,C.; Zahid,M.; Nemani,S.K.; Adnan,A.; Gudi,S.K.; Khanna,N.; Ebensen,Thomas; Lünsdorf,Heinrich; Guzmán,Carlos A.; Rinas,Ursula; (2013). Purification of hepatitis B surface antigen virus-like particles from recombinant Pichia pastoris and in vivo analysis of their immunogenic properties. Journal of Chromatography B: 940C, 104-111 DOI: 10.1016/j.jchromb.2013.09.030 HZI repository PubMed
  • Cano-Garrido,O.; Rodriguez-Carmona,E.; ez-Gil,C.; Vazquez,E.; Elizondo,E.; Cubarsi,R.; Seras-Franzoso,J.; Corchero,J.L.; Rinas,Ursula; Ratera,I.; Ventosa,N.; Veciana,J.; Villaverde,A.; Garcia-Fruitos,E.; (2013). Supramolecular organization of protein-releasing functional amyloids solved in bacterial inclusion bodies. Acta Biomaterialia: 9 4, 6134-614217427061 DOI: 10.1016/j.actbio.2012.11.033. WEBURLS
  • Buschart,A.; Gremmer,K.; El-Mowafy,M.; van den Heuvel,Joop; Müller,Peter P.; Bilitewski,Ursula; (2012). A novel functional assay for fungal histidine kinases group III reveals the role of HAMP domains for fungicide sensitivity. Journal of Biotechnology: 157 1, 268-277 PubMed
  • Chen,R.; John,J.; Lavrentieva,A.; Müller,S.; Tomala,M.; Zhao,Y.; Zweigerdt,R.; Beutel,S.; Hitzmann,B.; Kasper,C.; Martin,U.; Rinas,Ursula; Stahl,F.; Scheper,T.; (2012). Cytokine production using membrane adsorbers: Human basic fibroblast growth factor produced by Escherichia coli. Engineering in Live Sciences: 12 1, 29-38
  • Endres,S.; Hiebl,B.; Hägele,J.; Beltzer,C.; Fuhrmann,R.; Jäger,Volker; Almeida,M.; Costa,E.; Santos,C.; Traupe,H.; Jung,E.M.; Prantl,L.; Jung,F.; Wilkea,A.; Franke,R.-P.; (2012). Erratum: Angiogenesis and healing with non-shrinking, fast degradeable PLGA/CaP scaffolds in critical-sized defects in the rabbit femur with or without osteogenically induced mesenchymal stem cells (Clinical Hemorheology and Microcirculation (2011) 48:1 (. Clinical Hemorheology and Microcirculation: 49 1-4, 551
  • Vazquez,E.; Corchero,J.L.; Burgueno,J.F.; Seras-Franzoso,J.; Kosoy,A.; Bosser,R.; Mendoza,R.; Martinez-Lainez,J.M.; Rinas,Ursula; Fernandez,E.; Ruiz-Avila,L.; Garcia-Fruitos,E.; Villaverde,A.; (2012). Functional inclusion bodies produced in bacteria as naturally occurring nanopills for advanced cell therapies. Advanced Materials: 24 13, 1742-1747 PubMed
  • Kugler,J.; Schmelz,S.; Gentzsch,J.; Haid,S.; Pollmann,E.; van den Heuvel,Joop; Franke,R.; Pietschmann,Thomas; Heinz,Dirk W.; Collins,J.; (2012). High Affinity Peptide Inhibitors of the Hepatitis C Virus NS3-4A Protease Refractory to Common Resistant Mutants. Journal of Biological Chemistry: 287 46, 39224-39232 DOI: 10.1074/jbc.M112.393843 HZI repository PubMed
  • Sans,C.; Garcia-Fruitos,E.; Ferraz,R.M.; Gonzalez-Montalban,N.; Rinas,Ursula; Lopez-Santin,J.; Villaverde,A.; Alvaro,G.; (2012). Inclusion bodies of fuculose-1-phosphate aldolase as stable and reusable biocatalysts. Biotechnology progress: 28 2, 421-427 DOI: 10.1002/btpr.1518 HZI repository PubMed
  • Vanz,A.L.; Lünsdorf,Heinrich; Adnan,A.; Nimtz,Manfred; Gurramkonda,C.; Khanna,N.; Rinas,Ursula; (2012). Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: catabolic adaptation, stress responses, and autophagic processes. Microbial Cell Factories: 11, 103 PubMed
  • Iphofer,A.; Kummer,A.; Nimtz,Manfred; Ritter,A.; Arnold,T.; Frank,R.; van den Heuvel,Joop; Kessler,B.M.; Jänsch,Lothar; Franke,R.; (2012). Profiling ubiquitin linkage specificities of deubiquitinating enzymes with branched ubiquitin isopeptide probes. ChemBioChem: 13 10, 1416-1420 PubMed
  • Schmidt,G.; Krings,U.; Nimtz,Manfred*; Berger,R.G.; (2011). A surfactant tolerant laccase of Meripilus giganteus. World Journal of Microbiology and Biotechnology: 28 4, 1623-1632
  • Endres,S.; Hiebl,B.; Hagele,J.; Beltzer,C.; Fuhrmann,R.; Jäger,Volker*; Almeida,M.; Costa,E.; Santos,C.; Traupe,H.; Jung,E.M.; Prantl,L.; Jung,F.; Wilke,A.; Franke,R.P.; (2011). Angiogenesis and healing with non-shrinking, fast degradeable PLGA/CaP scaffolds in critical-sized defects in the rabbit femur with or without osteogenically induced mesenchymal stem cells. Clinical Hemorheology and Microcirculation: 48 1, 29-40 PubMed
  • Bals,C.; Schambach,A.; Meyer,J.; Scheper,T.; Rinas,Ursula*; (2011). Expression and purification of bioactive soluble murine stem cell factor from recombinant Escherichia coli using thioredoxin as fusion partner. Journal of Biotechnology: 152 1-2, 1-8 DOI: 10.1016/j.jbiotec.2011.01.012 HZI repository PubMed
  • Busso,D.; Peleg,Y.; Heidebrecht,T.; Romier,C.; Jacobovitch,Y.; Dantes,A.; Salim,L.; Troesch,E.; Schuetz,A.; Heinemann,U.; Folkers,G.E.; Geerlof,A.; Wilmanns,M.; Polewacz,A.; Quedenau,C.; Bussow,Konrad*; Adamson,R.; Blagova,E.; Walton,J.; Cartwright,J.L.; Bird,L.E.; Owens,R.J.; Berrow,N.S.; Wilson,K.S.; Sussman,J.L.; Perrakis,A.; Celie,P.H.; (2011). Expression of protein complexes using multiple Escherichia coli protein co-expression systems: A benchmarking study. Journal of Structural Biology: 175 2, 159-170 DOI: 10.1016/j.jsb.2011.03.004 HZI repository PubMed
  • Ergin,A.; Syrbe,U.; Scheer,R.; Thiel,A.; Adam,T.; Büssow,Konrad*; Duchmann,R.; Zeitz,M.; Sieper,J.; (2011). Impaired Peripheral Th1 CD4+ T Cell Response to Escherichia coli Proteins in Patients with Crohn's Disease and Ankylosing Spondylitis3529. Journal of Clinial Immunology: 31 6, 998-1009 PubMed
  • Gohler,A.K.; Kokpinar,O.; Schmidt-Heck,W.; Geffers,Robert*; Guthke,R.; Rinas,Ursula*; Schuster,S.; Jahreis,K.; Kaleta,C.; (2011). More than just a metabolic regulator - elucidation and validation of new targets of PdhR in Escherichia coli1. BMC Systems Biology: 5, 197 DOI: 10.1186/1752-0509-5-197 HZI repository PubMed
  • Jain,G.; Jayaraman,G.; Kökpinar,O.; Rinas,Ursula*; Hitzmann,B.; (2011). On-line monitoring of recombinant bacterial cultures using multi-wavelength fluorescence spectroscopy. Biochemical Engineering Journal: 58-59 1, 133-139
  • Li,Z.; Nimtz,Manfred*; Rinas,Ursula*; (2011). Optimized procedure to generate heavy isotope and selenomethionine-labeled proteins for structure determination using Escherichia coli-based expression systems. Applied Microbiology and Biotechnology: 92 4, 823-833 DOI: 10.1007/s00253-011-3603-x HZI repository PubMed
  • Gueorguieva,L.; Palani,S.; Rinas,Ursula*; Jayaraman,G.; Seidel-Morgenstern,A.; (2011). Recombinant protein purification using gradient assisted simulated moving bed hydrophobic interaction chromatography. Part II: Process design and experimental validation. Journal of Chromatography A: 1218 37, 6402-6411 PubMed
  • Palani,S.; Gueorguieva,L.; Rinas,Ursula*; Seidel-Morgenstern,A.; Jayaraman,G.; (2011). Recombinant protein purification using gradient-assisted simulated moving bed hydrophobic interaction chromatography. Part I: Selection of chromatographic system and estimation of adsorption isotherms. Journal of Chromatography A: 1218 37, 6396-6401 PubMed
  • Li,Z.; Kessler,Wolfgang*; van den,Heuvel Joop*; Rinas,Ursula*; (2011). Simple defined autoinduction medium for high-level recombinant protein production using T7-based Escherichia coli expression systems. Applied Microbiology and Biotechnology: 91 4, 1203-1213 DOI: 10.1007/s00253-011-3407-z HZI repository PubMed
  • Wilke,S.; Gröbe,Lothar*; Maffenbeier,V.; Jäger,Volker*; Gossen,M.; Josewski,J.; Duda,A.; Polle,L.; Owens,R.J.; Wirth,Dagmar*; Heinz,Dirk* W.; van den,Heuvel Joop*; Büssow,Konrad*; (2011). Streamlining homogeneous glycoprotein production for biophysical and structural applications by targeted cell line development. PLoS ONE: 6 12, e27829 DOI: 10.1371/journal.pone.0027829 HZI repository PubMed
  • de Groot,J.C.; Schluter,K.; Carius,Y.; Quedenau,C.; Vingadassalom,D.; Faix,J.; Weiss,S.M.; Reichelt,J.; Standfuss-Gabisch,C.; Lesser,C.F.; Leong,J.M.; Heinz,Dirk W.*; Büssow,Konrad*; Stradal,Theresia E.*; (2011). Structural Basis for Complex Formation between Human IRSp53 and the Translocated Intimin Receptor Tir of Enterohemorrhagic E. coli. 19 9, 1294-1306 DOI: 10.1016/j.str.2011.06.015 HZI repository PubMed
  • Lünsdorf,Heinrich*; Gurramkonda,C.; Adnan,A.; Khanna,N.; Rinas,Ursula*; (2011). Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen. Microbial Cell Factories: 10, 48 DOI: 10.1186/1475-2859-10-48 HZI repository PubMed
  • Gurramkonda,C.; Polez,S.; Skoko.N.; Adnan,A.; Gäbel,Th.; Chugh,D.; Swaminathan,S.; Khanna,N.; Tisminetzky,S.; Rinas,Ursula*; (2010). Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin.. Microbial Cell Factories: 12 9, 311475-2859 DOI: 10.1186/1475-2859-9-31 HZI repository PubMed
  • Wilke,S.; Krausze,J.; Gossen,M.; Gröbe,Lothar*; Jager,V.; Gherardi,E.; van den,Heuvel J.; Bussow,Konrad*; (2010). Glycoprotein production for structure analysis with stable, glycosylation mutant CHO cell lines established by fluorescence-activated cell sorting. Protein Science: 19 6, 1264-1271 PubMed
  • Martinez-Alonso,M.; Garcia-Fruitos,E.; Ferrer-Miralles,N.; Rinas,Ursula*; Villaverde,A.; (2010). Side effects of chaperone gene co-expression in recombinant protein production. Microbial Cell Factories: 9, 641475-2859 DOI: 10.1186/1475-2859-9-64 HZI repository PubMed
  • Lu,X.; Sun,J.; Nimtz,Manfred*; Wissing,J.; Zeng,A.-P.; Rinas,Ursula*; (2010). The intra- and extracellular proteome of Aspergillus niger growing on defined medium with xylose or maltose as carbon substrate. Microbial Cell Factories: 9 23, 1-13 DOI: 10.1186/1475-2859-9-23 HZI repository PubMed
  • Schliephake,H.; Zghoul,N.; Jäger,Volker*; van,Griensven M.; Zeichen,J.; Gelinsky,M.; Szubtarsky,N.; (2009). Bone formation in trabecular bone cell seeded scaffolds used for reconstruction of the rat mandible. International Journal of Oral and Maxillofacial Surgery Surgery: 38 2, 166-172 PubMed
  • Shiloach,J.; Rinas,Ursula*; (2009). Glucose and acetate metbolism in E. coli - System level analysis and biotechnological applications in protein producing processes.: Systems Biology and Biotechnology of E. coli. Lee,S.-Y.; (Ed.). Berlin, Heidelberg, New York : Springer Netherlands, 377-400 ISBN: 978-1-4020-9393-7 (print), 978-1-4020-9394-4 (online)
  • Wiesand,U.; Sorg,I.; Amstutz,M.; Wagner,S.; van den Heuvel,Joop*; Lührs,Thorsten*; Cornelis,G.R.; Heinz,Dirk W.*; (2009). Structure of the type III secretion recognition protein YscU from Yersinia enterocolitica. Journal of Molecular Biology: 385 3, 854-866 DOI: 10.1016/j.jmb.2008.10.034 HZI repository PubMed
  • Visser,R.; Arrabal,P.M.; Becerra,J.; Rinas,Ursula*; Cifuentes,M.; (2009). The effect of an rhBMP-2 absorbable collagen sponge-targeted system on bone formation in vivo. Biomaterials: 30 11, 2032-2037 PubMed
  • Schliephake,H.; Zghoul,N.; Jäger,Volker*; van,Griensven M.; Zeichen,J.; Gelinsky,M.; Wulfing,T.; (2008). Effect of seeding technique and scaffold material on bone formation in tissue-engineered constructs. Journal of Biomedical Materials Research - A: 90 2, 429-437 DOI: doi PubMed
  • Wollert,T.; Pasche,Bastian*; Rochon,Maike*; Deppenmeier,S.; van den Heuvel,Joop*; Gruber,A.D.; Heinz,Dirk W.*; Lengeling,Andreas*; Schubert,Wolf-Dieter*; (2007). Extending the host range of Listeria monocytogenes by rational protein design. Cell: 129 5, 891-902
  • Zieseniss,A.; Schroeder,U.; Buchmeier,S.; Schoenberger,C.; van den Heuvel,Joop*; Jockusch,B.; Illenberger,S.; (2007). Raver1 is an integral component of muscle contractile elements. Cell and Tissue Research: 327 3, 583-594 DOI: 10.1007/s00441-006-0322-1 HZI repository PubMed
  • Niemann,H.N.; Jäger,Volker*; Butler,P.J.G.; van den Heuvel,Joop*; Schmidt,S.; Ferraris,D.; Gherardi,E.; Heinz,Dirk W.*; (2007). Structure of the human receptor tyrosine kinase met in complex with the Listeria monocytogenes Invasion Protein InlB. Cell: 130 2, 235-246 DOI: 10.1016/j.cell.2007.05.037 HZI repository PubMed
  • Zheng,P; Sun,Jibin*; van den Heuvel,Joop*; Zeng,An-Ping*; (2006). Discovery and investigation of a new, second triose phosphate isomerase in Klebsiella pneumoniae. Journal of Biotechnology: 125 4, 462-473
  • Zheng,P.; Wereath,K.; Sun,Jibin*; van den Heuvel,Joop*; Zeng,An-Ping*; (2006). Overexpression of genes of the dha regulon and its effects on cell growth, glycerol fermentation to 1,3-propanediol and plasmid stability in Klebsiella pneumoniae. Process Biochemistry: 41 10, 2160-2169
  • Dresler,K.; van den Heuvel,Joop*; Müller,Rolf-Joachim*; Deckwer,Wolf-Dieter*; (2006). Production of a recombinant polyester-cleaving hydrolase from Thermobifida fusca in Escherichia coli. Bioprocess and Biosystems Engineering: 29, 169-183 WEBURLS
  • Kleeberg,I.; Welzel,K.; van den Heuvel,J.; Müller,R.-J.; Deckwer,W.-D.; (2005). Characterization of a new extracellular hydrolase from Thermobifida fusca degrading aliphatic-aromatic copolyesters. Biomacromolecules: 6, 262-270
  • Astner,I.; Schulze,J.O.; van den Heuvel,J.; Jahn,D.; Schubert,W.-D.; Heinz,D.W.; (2005). Crystal structure of 5-aminolevulinate synthase, the first enzyme of heme biosynthesis, and its link to XLSA in humans. Embo Journal: 24, 3166-3177 DOI: 10.1038/sj.emboj.7600792 HZI repository
  • Bollati-Fogolin,M.; Irani,N.; Beccaria,A.J.; Schulz,C.; van den Heuvel,J.; Elias,C.B.; Carpentier,E.; Durocher,Y.; Bisson,L.; Etcheverrigaray,M.; Kratje,R.B.; Wirth,M.; Kamen,A.; Wagner,R.; (2005). Impact of yeast pyruvate carboxylase on the productivity of animal host cell lines.: Animal Cell Technology Meets Genomics. Godia,F.; Fussenegger,M.; (Ed.). Dordrecht : Kluwer Academic Publishers, 87-89
  • Hoffmann,F.; van den Heuvel,J.; Zidek,N.; Rinas,U.; (2004). Minimizing inclusion body formation during recombiant protein production in Escherichia coli at bench and pilot plant scale.. Enzyme and Microbial Technology: 34, 235-241
  • Ross,A.; Kessler,W.; Krumme,D.; Menge,U.; Wissing,J.; van den Heuvel,J.; Flohé,L.; (2004). Optimised fermentation strategy for 13C/15N recombinant protein labelling in Escherichia coli for NMR-structure analysis. Journal of Biotechnology: 108, 31-39

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