Flow Cytometry and Cell Sorting

Our organism is made of many different cells that are specialized to perform specific tasks. As long as they work together in the collective, we will stay healthy – do they change or are they changed by pathogens, we become unbalanced and sick. The Flow Cytometry and Cell Sorting platform provides the instrumentation and expert knowledge for the phenotypic identification and functional analysis of cells at the single cell level.


Publications to the Topic Cytometry

Kahlisch L.; Henne K.; Gröbe L.; Brettar I.; Höfle MG. (2012) Assessing the viability of bacterial species in drinking water by combined cellular and molecular analyses. Microb Ecol.: 63(2):383-97.

Wilke, S.; Groebe, L.; Maffenbeier V.; Jäger V.; Gossen M.; Josewski J.; Duda A.; Polle L.; Owens RJ.; Wirth D.; Heinz DW.; van den Heuvel J.; Büssow K. (2011) Streamlining homogeneous glycoprotein production for biophysical and structural applications by targeted cell line development. PLoS One: 6(12) e27829.

Zygmunt BM.; Groebe L.; Guzman CA. (2011) Peritoneal cavity is dominated by IFNγ-secreting CXCR3+ Th1 cells. PLoS One: 6(7):e18032.

Lemme A.; Gröbe L.; Reck M.; Tomasch J.; Wagner-Döbler I. (2011) Subpopulation-specific transcriptome analysis of competence-stimulating-peptide-induced Streptococcus mutans. J Bacteriol.: 193(8):1863-77.

Klippel N.; Cui S.; Groebe L.; Bilitewski U. (2010) Deletion of the Candida albicans histidine kinase gene CHK1 improves recognition by phagocytes through an increased exposure of cell wall beta-1,3-glucans. Microbiology: 156(Pt11):3432-44.

Wilke S.; Krausze J.; Gossen M.; Groebe L.; Jäger V.; Gherardi E.; van den Heuvel J.; Büssow K. (2010) Glycoprotein production for structure analysis with stable, glycosylation mutant CHO cell lines established by fluorescence-activated cell sorting. Protein Sci.: 19(6):1264-71.

Kahlisch L.; Henne K.; Groebe L.; Draheim J.; Höfle MG.; Brettar I. (2010) Molecular analysis of the bacterial drinking water community with respect to live/dead status. Water Sci Technol.: 61(1):9-14.

Pils MC.; Pisano F.; Fasnacht N.; Heinrich JM.; Groebe L.; Schippers A.; Rozell B.; Jack RS.; Müller W. (2010) Monocytes/macrophages and/or neutrophils are the target of IL-10 in the LPS endotoxemia model. Eur J Immunol.: 40(2):443-8.

Zygmunt BM.; Rharbaoui F.; Groebe L.; Guzman CA. (2009) Intranasal immunization promotes th17 immune responses. J Immunol.: 183(11):6933-8.

Madan R.; Demircik F.; Surianarayanan S.; Allen JL.; Divanovic S.; Trompette A.; Yogev N.; Gu Y.; Khodoun M.; Hildeman D.; Boespflug N.; Fogolin MB.; Gröbe L.; Greweling M.; Finkelman FD.; Cardin R.; Mohrs M.; Müller W.; Waisman A.; Roers A.; Karp CL. (2009) Nonredundant roles for B cell-derived IL-10 in immune counter-regulation. J Immunol.: 183(4):2312-20.

Westendorf AM.; Fleissner D.; Groebe L.; Jung S.; Gruber AD.; Hansen W.; Buer J. (2009) CD4+Foxp3+ regulatory T cell expansion induced by antigen-driven interaction with intestinal epithelial cells independent of local dendritic cells. Gut: 58(2):211-9.

Gekara NO.; Groebe L.; Viegas N.; Weiss S. (2008) Listeria monocytogenes desensitizes immune cells to subsequent Ca2+ signaling via listeriolysin O-induced depletion of intracellular Ca2+ stores. Infect Immun.: 76(2):857-62.

Hansen W.; Westendorf AM.; Reinwald S.; Bruder D.; Deppenmeier S.; Groebe L.; Probst-Kepper M.; Gruber AD.; Geffers R.; Buer J. (2007) Chronic antigen stimulation in vivo induces a distinct population of antigen-specific Foxp3 CD25 regulatory T cells. J Immunol.: 179(12):8059-68. Erratum in: J Immunol.: 181(8):5803.

Gereke M.; Gröbe L.; Prettin S.; Kasper M.; Deppenmeier S.; Gruber AD.; Enelow RI.; Buer J.; Bruder D. (2007) Phenotypic alterations in type II alveolar epithelial cells in CD4+ T cell mediated lung inflammation. Respir Res.: 8,47.

Fleige A.; Alberti S.; Gröbe L.; Frischmann U.; Geffers R.; Müller W.; Nordheim A.; Schippers A. (2007) Serum response factor contributes selectively to lymphocyte development. J Biol Chem.: 282(33):24320-8.

Gekara NO.; Westphal K.; Ma B.; Rohde M.; Groebe L.; Weiss S. (2007) The multiple mechanisms of Ca2+ signalling by listeriolysin O, the cholesterol-dependent cytolysin of Listeria monocytogenes. Cell Microbiol.: 9(8):2008-21.

Ocklenburg F.; Moharregh-Khiabani D.; Geffers R.; Janke V.; Pfoertner S.; Garritsen H.; Groebe L.; Klempnauer J.; Dittmar KE.; Weiss S.; Buer J.; Probst-Kepper M. (2006) UBD, a downstream element of FOXP3, allows the identification of LGALS3, a new marker of human regulatory T cells. Lab Invest.: 86(7):724-37.

Further Publications

Pistor S.; Gröbe L.; Sechi AS.; Domann E.; Gerstel B.; Machesky LM.; Chakraborty T.; Wehland J. (2000) Mutations of arginine residues within the 146-KKRRK-150 motif of the ActA protein of Listeria monocytogenes abolish intracellular motility by interfering with the recruitment of the Arp2/3 complex. J Cell Sci.: 113 ( Pt 18):3277-87.

Laurent V.; Loisel TP.; Harbeck B.; Wehman A.; Gröbe L.; Jockusch BM.; Wehland J.; Gertler FB.; Carlier MF. (1999) Role of proteins of the Ena/VASP family in actin-based motility of Listeria monocytogenes. J Cell Biol.: 144(6):1245-58.

Gerstel B.; Gröbe L.; Pistor S.; Chakraborty T.; Wehland J. (1996) The ActA polypeptides of Listeria ivanovii and Listeria monocytogenes harbor related binding sites for host microfilament proteins. Infect Immun.: 64(6):1929-36.

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