Flow Cytometry and Cell Sorting

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Our organism is made of many different cells that are specialized to perform specific tasks. As long as they work together in the collective, we will stay healthy – do they change or are they changed by pathogens, we become unbalanced and sick. The Flow Cytometry and Cell Sorting platform provides the instrumentation and expert knowledge for the phenotypic identification and functional analysis of cells at the single cell level.

Cell Sorting

As an extension of analytical flow cytometry is cell sorting used to separate specific cell types from a heterogeneous population. The sorted cells can be used for cell culture, DNA analysis, RNA analysis, microscopy, protein extraction. Projects involve the sorting of highly pure cell populations, like naïve and regulatory T cells, NK cells and other cell types of the immune system. The sorted cells are used for in-vitro assays and adoptive transfer experiments. A very common application is the purification of rare cells for subsequent gene expression profiling. Another application is the repetitive sorting of fluorescent protein transfected cells to facilitate the generation of stable expressing cell lines.

Instruments

We currently have several flow cytometers with different performances available

Cytometer:

  • BD LSR-II/Fortessa
    5 Laser (360, 405, 488, 561, 640nm)
    18 fluorescence colours
  • BD LSR-II
    3 Laser (405, 488, 633nm)
    11 fluorescence colours
  • BD FACSCanto, FACSCalibur and Accuri C6
    2 laser (488, 633nm)
    4-6 fluorescence colurs

 

Cell sorter:

  • BD FACSAria-II
    3 Laser (405, 488, 633nm)
    9 fluorescence colours
  • BD FACSAria-II SORP
    4 Laser (405, 488, 561, 640nm)
    13 fluorescence colours
  • BC MoFlo XDP
    4 Laser (405, 488, 561, 640nm)
    9 fluorescence colours

Leader

  • Dr Lothar Gröbe

    Lothar Groebe

    Head of Platform Flow Cytometry and Cell Sorting

    +49 531 6181-3079

    +49 531 6181-3399

    Contact