To modify the genome of mice we employ state of the art technologies of molecular biology. Genetic engineering comprises the introduction of point mutations and tags into the mouse genome, modifications for constitutive and tissue specific (conditional) inactivation of genes (knock-out), the targeted integration of reporters or recombinases (knock-in) and finally also the integration of synthetic cassettes for externally controlled expression of genes.
The following tools are routinely used
- classical strategies for homologous recombination
- Crispr/Cas9 based homologous recombination and generation of knock-outs
- Targeting of chromosomal ‘hot-spots’ with the help of sequence specific recombinases (recombinase mediated cassette exchange RMCE)
- Engineering of bacterial artificial chromosomes (BACs)
To generate genetically modified mice two different strategies routes are followed
- modification of embryonic stem cells, followed by subsequent implementation in blastocysts and transfer into founder mice
- modification of zygotes and subsequent transfer into founder mice.
We develop tools for predictable and externally controlled gene expression. To this end, we investigate the interaction of introduced (synthetic) cassettes with the host’s flanking chromosomal environment. Further, we investigate the mechanisms leading to transgene inactivation and develop methods to overcome silencing.
Bachelor & Master
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