Structural Biology of Autophagy

Cells continuously synthesize proteins, reconstruct their organelles and cellular components, renew them and take up substances from outside. A cellular mechanism that counteracts and controls this on-going growth of organic matter is autophagy. This particular mechanism degrades everything that is no longer needed – and this includes pathogens as well. In this regard autophagy plays a central role in our immune response and the defence against infectious diseases. Here you can find out how our scientists are researching autophagy in order to fight against pathogens and infectious diseases.


Selected Publications

Scrima A, Wittinghofer A (2006) Dimerisation-dependent GTPase reaction of MnmE: how potassium acts as GTPase-activating element. EMBO J 25: 2940-51

Scrima A, Vetter IR, Armengod ME, Wittinghofer A (2005) The structure of the TrmE GTP-binding protein and its implications for tRNA modification. EMBO J 24: 23-33

Scrima A, Konickova R, Czyzewski BK, Kawasaki Y, Jeffrey PD, Groisman R, Nakatani Y, Iwai S, Pavletich NP, Thomä NH (2008) Structural basis of UV DNA-damage recognition by the DDB1-DDB2 complex. Cell 135: 1213-23

Fischer ES, Scrima A, Böhm K, Matsumoto S, Lingaraju GM, Faty M, Yasuda T, Cavadini S, Wakasugi M, Hanaoka F, Iwai S, Gut H, Sugasawa K, Thomä NH (2011) The molecular basis of CRL4DDB2/CSA ubiquitin ligase architecture, targeting, and activation. Cell 147: 1024-39

Fiebig D*, Schmelz S*, Zindel S, Ehret V, Beck J, Ebenig A, Ehret M, Fröls S, Pfeifer F, Kolmar H, Fuchsbauer HL*, Scrima A* (2016) Structure of the Dispase Autolysis-inducing Protein from Streptomyces mobaraensis and Glutamine Cross-linking Sites for Transglutaminase. J Biol Chem 291: 20417-26


  • Authors: Archna,A.; Scrima,A.; ( Year: 2017). Title: Identification, biochemical characterization and crystallization of the central region of human ATG16L1. Journal: Acta Crystallogr.F.Struct.Biol.Commun. Volume: 73 Issue: Pt 10, S-Page: 560- E-Page: 567 DOI: 10.1107/S2053230X17013280 PubMed
  • Authors: Leupold,S.; Büsing,P.; Mas,P.J.; Hart,D.J.; Scrima,A.; ( Year: 2017). Title: Structural insights into the architecture of the Shigella flexneri virulence factor IcsA/VirG and motifs involved in polar distribution and secretion. Journal: J.Struct.Biol. Volume: 198 Issue: 1, S-Page: 19- E-Page: 27 DOI: 10.1016/j.jsb.2017.03.003 PubMed
  • Authors: Bajagic,M.; Archna; Busing,P.; Scrima,A.; ( Year: 2017). Title: Structure of the WD40-domain of human ATG16L1. Journal: Protein Sci. Volume: 26 Issue: 9, S-Page: 1828- E-Page: 1837 DOI: 10.1002/pro.3222 PubMed
  • Authors: Sadana,P.; Monnich,M.; Unverzagt,C.; Scrima,A.; ( Year: 2017). Title: Structure of the Y. pseudotuberculosis adhesin InvasinE. Journal: Protein Sci. Volume: 26 Issue: 6, S-Page: 1182- E-Page: 1195 DOI: 10.1002/pro.3171 Fulltext HZI repository PubMed
  • Authors: Bleckmann,M.; Schmelz,S.; Schinkowski,C.; Scrima,A.; van den,Heuvel J.; ( Year: 2016). Title: Fast plasmid based protein expression analysis in insect cells using an automated SplitGFP screen. Journal: Biotechnol.Bioeng. Volume: 113 Issue: 9, S-Page: 1975- E-Page: 1983 DOI: 10.1002/bit.25956 HZI repository PubMed
  • Authors: Kühne,C.; Singer,H.M.; Grabisch,E.; Codutti,L.; Carlomagno,T.; Scrima,A.; Erhardt,M.; ( Year: 2016). Title: RflM mediates target specificity of the RcsCDB phosphorelay system for transcriptional repression of flagellar synthesis in Salmonella enterica. Journal: Molecular Microbiology Volume: 101 Issue: 5, S-Page: 841- E-Page: 855 DOI: 10.1111/mmi.13427 HZI repository PubMed
  • Authors: Fiebig,D.; Schmelz,S.; Zindel,S.; Ehret,V.; Beck,J.; Ebenig,A.; Ehret,M.; Fröls,S.; Pfeifer,F.; Kolmar,H.; Fuchsbauer,H.L.; Scrima,A.; ( Year: 2016). Title: Structure of the dispase autolysis-inducing protein from Streptomyces mobaraensis and glutamine cross-linking sites for transglutaminase. Journal: J.Biol.Chem. Volume: 291 Issue: 39, S-Page: 20417- E-Page: 20426 DOI: Article WEBURLS
  • Authors: Raupach-Rosin,H.; Klett-Tammen,C.J.; Schmalz,O.; Karch,A.; Castell,S.; Mikolajczyk,R.; ( Year: 2015). Title: ["I cannot kiss my wife"- An Analysis of Daily Experiences of MRSA-carriers]. Journal: Gesundheitswesen Volume: 78, S-Page: 822- E-Page: 827 PubMed
  • Authors: Haufschildt,K.; Schmelz,Stefan; Kriegler,T.M.; Neumann,A.; Streif,J.; Arai,H.; Heinz,Dirk W.; Layer,G.; ( Year: 2014). Title: The Crystal Structure of Siroheme Decarboxylase in Complex with Iron-Uroporphyrin III Reveals Two Essential Histidine Residues. Journal: Journal of Molecular Biology PubMed
  • Authors: Hofmeyer,T.; Schmelz,S.; Degiacomi,M.T.; Dal Peraro,M.; Daneschdar,M.; Scrima,Andrea; van den Heuvel,Joop; Heinz,Dirk W.; Kolmar,H.; ( Year: 2013). Title: Arranged sevenfold: Structural insights into the C-terminal oligomerization domain of human C4b-binding protein. Journal: Journal of Molecular Biology Volume: 425 Issue: 8, S-Page: 1302- E-Page: 1317 DOI: 10.1016/j.jmb.2012.12.017 HZI repository PubMed
  • Authors: Shi,T.; Bunker,R.D.; Mattarocci,S.; Ribeyre,C.; Faty,M.; Gut,H.; Scrima,Andrea; Rass,U.; Rubin,S.M.; Shore,D.; Thomä,N.H.; ( Year: 2013). Title: Rif1 and Rif2 shape telomere function and architecture through multivalent Rap1 interactions. Journal: Cell Volume: 153 Issue: 6, S-Page: 1340- E-Page: 1353 WEBURLS
  • Authors: Scrima A, Fischer ES, Lingaraju GM, Böhm K, Cavadini S, Thomä NH.; ( Year: 2011). Title: FEBS Lett. Detecting UV-lesions in the genome: The modular CRL4 ubiquitin ligase does it best!. PubMed
  • Authors: Fischer,E.S.; Scrima,Andrea*; Bohm,K.; Matsumoto,S.; Lingaraju,G.M.; Faty,M.; Yasuda,T.; Cavadini,S.; Wakasugi,M.; Hanaoka,F.; Iwai,S.; Gut,H.; Sugasawa,K.; Thoma,N.H.; ( Year: 2011). Title: The Molecular Basis of CRL4(DDB2/CSA) Ubiquitin Ligase Architecture, Targeting, and Activation. Journal: Cell Volume: 147 Issue: 5, S-Page: 1024- E-Page: 1039 PubMed
  • Authors: Meyer S, Scrima A, Versées W, Wittinghofer A.; ( Year: 2008). Title: Crystal structures of the conserved tRNA-modifying enzyme GidA: implications for its interaction with MnmE and substrate.. Journal: J Mol Biol Issue: 380(3), S-Page: 532- E-Page: 547 PubMed
  • Authors: Scrima A, Thomas C, Deaconescu D, Wittinghofer A.; ( Year: 2008). Title: The Rap-RapGAP complex: GTP hydrolysis without catalytic glutamine and arginine residues.. Journal: EMBO J. Issue: 27(7), S-Page: 1145- E-Page: 1153 PubMed
  • Authors: Thomas C, Fricke I, Scrima A, Berken A, Wittinghofer A; ( Year: 2007). Title: Structural Evidence for a Common Intermediate in Small G Protein-GEF Reactions.. Journal: Mol Cell Issue: 25(1), S-Page: 141- E-Page: 149 PubMed
  • Authors: Scrima A, Wittinghofer A; ( Year: 2006). Title: Dimerisation-dependent GTPase reaction of MnmE: how potassium acts as GTPase-activating element. Journal: EMBO J. Issue: 25(12), S-Page: 2940- E-Page: 2951 PubMed
  • Authors: Gasper R, Scrima A, Wittinghofer A; ( Year: 2006). Title: Structural insights into HypB, a GTP-binding protein that regulates metal binding.. Journal: J Biol Chem Issue: 281(37), S-Page: 27492- E-Page: 27502 PubMed
  • Authors: Lammers M, Rose R, Scrima A, Wittinghofer A; ( Year: 2005). Title: The regulation of mDia1 by autoinhibition and its release by Rho*GTP. Journal: EMBO J Issue: 24(23), S-Page: 4176- E-Page: 4187 PubMed
  • Authors: Scrima A, Vetter IR, Armengod ME, Wittinghofer A; ( Year: 2005). Title: The structure of the TrmE GTPbindingprotein and its implications for tRNA modification. Journal: EMBO J Issue: 24(1), S-Page: 23- E-Page: 33 PubMed
PrintSend per emailShare