Model Systems for Infection and Immunity

In infection research experiments on cells and mice have a pivotal role. Frequently such model systems have to be tailored to allow tackling a specific scientific question. To this end, genetic modification of cell lines and mice is being pursued. 

Selected Publications

  • Alkharsah,K.R.; Singh,V.V.; Bosco,R.; Santag,S.; Grundhoff,A.; Konrad,A.; Sturzl,M.; Wirth,Dagmar*; Dittrich-Breiholz,O.; Kracht,M.; Schulz,T.F.; (2011). Deletion of Kaposi's sarcoma-associated herpesvirus FLICE inhibitory protein, vFLIP, from the viral genome compromises the activation of STAT1-responsive cellular genes and spindle cell formation in endothelial cells. Journal of Virology: 85 19, 10375-10388 PubMed
  • Schucht,R.; Coroadinha,A.S.; Zanta-Boussif,M.A.; Verhoeyen,E.; Carrondo,M.J.T.; Hauser,Hansjörg*; Wirth,Dagmar*; (2006). A new generation of retroviral producer cells: Predictable and stable virus production by Flp mediated site-specific integration of retroviral vectors. Molecular therapy: 14, 285-292 DOI: 10.1016/j.ymthe.2005.12.003 HZI repository PubMed
  • May,T.; Hauser,Hansjörg*; Wirth,Dagmar*; (2006). Current status of transcriptional regulation systems. Cytotechnology: 50, 109-119
  • Kues,W.A.; Schwinzer,R.; Wirth,Dagmar*; Verhoeyen,E.; Lemme,E.; Herrmann,D.; Barg-Kues,B.; Hauser,Hansjörg*; Wonigkeit,K.; Niemann,H.; (2006). Epigenetic silencing and tissue independent expression of a novel tetracycline inducible system in double transgenic pigs. FASEB journal: 20, 1200-1202
  • May,T.; Müller,P.; Weich,H.; Froese,N.; Deutsch,U.; Wirth,D.; Kröger,A.; Hauser,H.; (2005). Establishment of murine cell lines by constitutive and conditional immortalization. Journal of Biotechnology: 120 1, 99-110
  • Thiefes,A.; Wolter,S.; Mushinski,J.S.; Hoffmann,E.; Dittrich-Breiholz,O.; Graue,N.; Dörrie,A.; Schneider,H.; Wirth,D.; Luckow,B.; Resch,K.; Kracht,M.; (2005). Simultaneous blockade of NFkappaB, JNK and p38 MAPK by a kinase-inactive mutant of the protein kinase TAK1 sensitizes cells to apoptosis and affects a distinct spectrum of TNF target genes. Journal of Biological Chemistry: 280, 27728-27741
  • May,T.; Wirth,D.; Hauser,H.; Müller,P.P.; (2005). Transcriptionally regulated immortalization overcomes side effects of tmeperature-sensitive SV40 large T antigen. Biochemical and Biophysical Research Communications: 327 3, 734-741
  • May,T.; Lindenmaier,W.; Wirth,D.; Müller,P.P.; (2004). Application of a reversible immortalisation system for the generation of proliferation-controlled cell lines. Cytotechnology: 46, 69-78
  • Wirth,Dagmar; Hauser,Hansjörg; (2004). Flp-mediated integration of expression cassettes into FRT tagged chromosomal loci.: Recombinant Gene Expression : Reviews and Protocols. Balbás,Paulina; Lorence,Argelia; (Ed.). Totowa, NJ : Humana Press, 467-476 ISBN: 1-58829-262-2
  • Weissenbach,M.; Clahsen,T.; Weber,C.; Spitzer,D.; Wirth,D.; Vestwever,D.; Heinrich,P.C.; Schaper,F.; (2004). Interleukin-6 is a direct mediator of T cell migration. European Journal of Immunology: 34, 2895-2906
  • Unsinger,J.; Lindenmaier,W.; May,T.; Hauser,H.; Wirth,D.; (2004). Stable and strictly controlled expression of LTR-flanked autoregulated expression cassettes upon adenoviral transfer. Biochemical and Biophysical Research Communications: 319, 879-887
  • May,T.; Hauser,H.; Wirth,D.; (2004). Transcriptional control of SV40 expression allows a complete reversion of immortalization. Nucleic Acids Research: 32, 5529-5538
  • Spitzer,D.; Dittmar,K.E.; Rohde,M.; Hauser,H.; Wirth,D.; (2003). Green fluorescent protein-tagged retroviral envelope protein for analysis of virus-cell interactions.. Journal of Virology: 77, 6070-6075
  • Hirschmann,F.; Verhoeyen,E.; Wirth,D.; Bauwens,S.; Hauser,H.; Rudert,M.; (2002). Vital marking of articular chondrocytes by retroviral infection using green fluorescence protein. Osteoarthritis and Cartilage: 10 2, 109-118 ISI
  • Niemann,H.; Verhoeyen,E.; Wonigeit,K.; Lorenz,R.; Hecker,J.; Schwinzer,R.; Hauser,H.; Kues,W.A.; Halter,R.; Lemme,E.; Herrmann,D.; Winkler,M.; Wirth,D.; Paul,D.; (2001). Cytomegalovirus early promoter induced expression of hCD59 in porcine organs provides protection against hyperacute rejection. Transplantation: 72 12, 1898-19060041-1337 PubMed
  • Verhoeyen,E.; Hauser,H.; Wirth,D.; (2001). Evaluation of retroviral vector design in defined chromosomal loci by Flp-mediated cassette replacement. Human gene therapy: 12 8, 933-9441043-0342 PubMed
  • Unsinger,J.; Kroger,A.; Hauser,H.; Wirth,D.; (2001). Retroviral vectors for the transduction of autoregulated, bidirectional expression cassettes. Molecular therapy: 4 5, 484-4891525-0016 PubMed
  • Hauser,H.; Spitzer,D.; Verhoeyen,E.; Unsinger,J.; Wirth,D.; (2000). New approaches towards ex vivo and in vivo gene therapy. Cells Tissues Organs: 167 2-3, 75-801422-6405 PubMed
  • Spitzer, D.; Hauser, H.; Wirth, D. ; (1999). Complement-protected amphotropic retroviruses from murine packaging cells.. Hum Gene Ther: 10, 1893-1902
  • Herbst, A., Koester, M., Wirth, D., Hauser, H., Welte, K.; (1999). G-CSF receptor mutations in patients with severe congenital neutropenia do not abrogate Jak2 activation and stat1/stat3 translocation.. Ann N Y Acad Sci: 872, 325-327
  • Klehr-Wirth, D., Kuhnert, F., Unsinger, J., Hauser, H.; (1997). Generation of mammalian cells with conditional expression of cre recombinase.. Technical Tips Online: 2 1, 18-20
  • Bode, J., Schlake, T., Rios-Ramirez, M., Mielke, C., Stengert, M., Kay, V., Klehr-Wirth, D. ; (1995). Scaffold/matrix-attached regions: structural properties creating transcriptionally active loci.. Int Rev Cytol: 162A, 389-454
  • Schlake, T., Klehr-Wirth, D., Yoshida, M., Beppu, T., Bode, J. ; (1994). Gene expression within a chromatin domain: the role of core histone hyperacetylation.. Biochemistry: 33, 4197-4206
  • Wirth, M., Grannemann, R., Klehr, D., Hauser, H.; (1994). Screening retroviral packaging cells for highly efficient virus production by using a combined selection procedure.. J Virol: 68, 566-569
  • Bode, J., Kohwi, Y., Dickinson, L., Joh, T., Klehr, D., Mielke, C., Kohwi-Shigematsu, T.; (1992). Biological significance of unwinding capability of nuclear matrix-associating DNAs.. Science: 255, 195-197
  • Klehr, D., Schlake, T., Maass, K., Bode, J.; (1992). Scaffold-attached regions (SAR elements) mediate transcriptional effects due to butyrate.. Biochemistry: 31, 3222-3229
  • Klehr, D., Maass, K., Bode, J.; (1991). Scaffold-attached regions from the human interferon beta domain can be used to enhance the stable expression of genes under the control of various promoters.. Biochemistry: 30, 1264-1270
  • Bode, J., Klehr, D., Maass, K., Mielke, C. ; (1990). MARs and SARs, DNA sequences with an affinity to the nuclear matrix: analysis, regulation and functions in transcriptional enhancement.. BioEngineering: New Trends in Genetics: 2, 2-5
  • Klehr, D. and Bode, J. ; (1988). Comparative evaluation of bovine papilloma virus (BPV) vectors for the study of gene expression in mammalian cells.. Mol. Gen. (Life Sci Adv): 7, 47-52


  • Prof Dr Dagmar Wirth

    Dagmar Wirth

    Head of the Research Group Model Systems for Infection and Immunity

    +49 531 6181-5040

    +49 531 6181-5002


    CV and Publications

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