Cellular Proteome Research

Pathogenic bacteria, viruses and fungi utilize and alter physiological cellular processes of the infected host. Our studies focus on the identification of unknown processes in complex and molecular networks, which are involved in infectious diseases.

Leader

Mass spectrometry

Life is only possible when thousands of molecules like DNA, proteins, lipids and carbohydrates cooperate inside an organism in a goal-oriented way. Mass spectrometry (MS) is one of the most important analytical techniques, which allows a detailed characterization of these components that are essential for life and for studying their interplay.

The MS technique allows the determination of molecular masses of complex biological macromolecules like proteins or complex carbohydrates. With the help of biochemical methods and after interfacing with a chromatographic system, the targeted fragmentation of larger molecules and the determination of the masses of all fragments are sufficient for the deduction of the total protein content of bacterial, animal or human cells, and the elucidation of their primary structure and relative abundance.

The group is equipped with several high performance Mass spectrometers.

Additionally, a QTOF and an ion trap for HPLC- and GC-MS analyses, respectively, are available.

Main areas of research are:

  • comparative and quantitative analyses of „healthy“ and „diseased“ tissue;
  • search for biomarkers to allow a fast and anticipatory diagnosis of threatening diseases;
  • targeted identification of proteins that are putatively related to interesting biological phenomena;
  • detection und characterization of posttranslational modifications as phosphorylation and glycosylation with the background of cellular signaling cascades;
  • structural characterization of new low molecular weight molecules with interesting medical properties (e.g. new antibiotics).

Contact: 

  • Dr. Manfred Nimtz
  • Staff: Andrea Abrahamik, Anja Meier
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