Structure and Function of Proteins

The staff in the field of Structural Biology investigates the spatial structure and function of individual molecules. In this they utilise modern technologies such as x-ray structure analysis, nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry. Analysis is undertaken of both biomacromolecules (peptides, proteins, nucleic acids, etc.) and low molecular natural substances.

    PD Dr. Thomas Böldicke



    +49 531 6181-5050


    Selected Publications

    Böldicke, T.W., Hillenbrandt, G., Lanka, E., Staudenbauer, W.L. (1981) Rifampicin Resistant Initiation of DNA Synthesis on the Isolated Strands of Col E Plasmid DNA. Nucleic Acids Res. 9, 5215 – 5231

    Maywald, F., Böldicke, T., Gross, G., Frank, R., Blöcker, H., Meyerhans, A., Schwellnus, K., Ebbers, J., Bruns, W., Reinhardt, G., Schnabel, E., Schröder  W., Fritz, H., Collins, J. (1988) Human Pancreatic Trypsin Inhibitor (PSTI) Expressed in Active Form and Secreted from Escherichia coli. Gene. 68, 357 – 369.

    Böldicke, T., Kindt, S., Maywald, F., Fitzlaff, G., Böcher, M., Frank, R., Collins, J. (1988) Production of Specific Monoclonal Antibodies Against the Active Sites of Human Pancreatic Secretory Trypsin Inhibitor Variants by in vitro Immunization with Synthetic Peptides. Eur. J. Biochem. 175, 259 – 264.

    Uthoff, S., Böldicke,T. (1993) In vitro Immunization of Mouse Spleen Cells for the Production of Monoclonal IgG1 Antibodies Using an Antigen-Specific T Helper Cell Clone (D.10.G4.1). J. Immunol. Methods. 166, 165 – 175.

    Böldicke, T., Haase, B., Böcher, M., Lindenmaier, W. (1995) Human Monoclonal Antibodies to Cytomegalovirus. Characterization and Recombinant Expression of a Glycoprotein-B-Specific Antibody. Eur. J. Biochem. 234, 397 – 405.

    Böcher, M., Böldicke, T., Kieß, M., Bilitewski, U. (1997) Synthesis of Mono- and Bifunctional  Peptide-Dextran Conjugates for the Immobilization of Peptide Antigens on ELISA Plates: Properties and Applications. J. Immunol. Methods. 208, 191-202.

    Böldicke, T., Struck, F., Schaper, F., Tegge, W., Sobek, H., Villbrandt, B., Lankenau, P. and Böcher, M. (2000) A new peptide-affinity tag for the detection and affinity purification of recombinant proteins with a monoclonal antibody. J. Immunol. Methods. 240, 165-183.

    Böldicke, T., Tesar, M., Griesel, C., Rohde, M., Gröne, H.-J., Waltenberger J., Kollet, O., Lapidot, T., Yayon, A. and Weich, H. (2001) Single-chain antibodies recognizing the human vascular endothelial growth factor receptor-2 (VEGFR-2, flk-1) on the surface of primary endothelial cells and preselected CD34+ cells from cord blood. STEM CELLS. 19, 24-36.

    Böldicke, T., Weber, H., Mueller, P.P., Barleon, B., Bernal, M. (2005) Novel highly efficient intrabody mediates complete inhibition of cell surface expression of the human vascular endothelial growth factor receptor-2 (VEGFR-2/KDR). J. Immunol. Methods. 300, 146-159.

    Böldicke, T. (2007) Blocking translocation of cell surface molecules from the ER to the cell surface by intracellular antibodies targeted to the ER. J. Cell.Mol.Med. 11 (1), 54-70.

    Kirschning, C.J., Dreher, S., Maaß, B., Fichte, S., Schade, J., Schade, J., Köster, M., Noack, A., Lindenmaier, W., Wagner, H. Böldicke, T. (2010) Generation of anti-TLR2 intrabody mediating inhibition of macrophage surface TLR2 expression and TLR2-driven cell activation. BMC Biotechnol. 10:31.   

    Böldicke, T., Somplatzki, S., Sergeev, G., Mueller P.P. (2012) Functional inhibition of transitory proteins by intrabody-mediated retention in the endoplasmatic reticulum. Methods 56(3):338-50

    Kopacek, A., Böldicke, T., Lergenmüller S., Berthold, F., Jensen, M., Müller, P.P., Grosse-Hovest, L. (2013) Construction, expression and binding specificity of bispecific CD3 x VEGFR-2 and CD3 x NCAM antibodies in the single chain and diabody format. Advances in Bioscience and Biotechnolgy. 4, 654-664.

    Reimer, E., Somplatzki, S., Zegenhagen, D., Hänel S., Fels, A., Bollhorst, T., Grosse-Hovest, L., Bauer, S., Kirschning, C.J., Böldicke, T. (2013) Molecular cloning and characterization  of a novel anti-TLR9 intrabody. Cell. Mol. Biol. Lett. DOI: 10.2478

    Marschall, A.L.J. , Dübel S, Böldicke, T. (2015) Specific in vivo knockdown of protein function by intrabodies. mABS. 7: 1010-1035.

    Böldicke, T. (2015) Protein Targeting Compounds. Prediction, Selection and Activity of Specific Inhibitors. Springer Heidelberg, New York: 1-288. ISBN 978-3-319-22472-5.

    Marschall, A.L.J., Dübel, S., Böldicke, T. (2015) Recent Advances with ER Targeted Intrabodies. in: Böldicke, T., (ed.), Protein Targeting Compounds. Prediction, Selection and Activity of Specific Inhibitors. Springer ISBN 978-3-319-22472-5, p 77- 93

    Böldicke, T.  (2015) ER Intrabodies: Potent molecules for specific knockdown of proteins passing the ER. J Cell Sci Ther 6:4.

    Backhaus, O. Böldicke, T. (2016) ER-targeted Intrabodies Mediating Specific In Vivo Knockdown of Transitory Proteins in Comparison to RNAi. in: Abdurakhmonov, I. Y., (ed.), RNA Interference. Intech ISBN 978-953-51-2272-2, p. 137 – 161



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