Molecular Cell Biology


The cytoskeleton is responsible for the mechanical stabilisation of the cell and its outer shape, for the movement of the cell as a whole as well as for movement and transportation within the cell. The research group "XY" focusses on one specific part of the cytoskeleton: the aktincytoskeleton. Which plays a role in giving an effective immune response as well as a target for pathogens. Understanding the molecular mechanisms underlining the construction and destruction of the actin structure is one of the main goals of Klemens Rottner and his teams.

Publications

Steffen, A., Stradal, T.E., Rottner, K. (2016) Signalling Pathways Controlling Cellular Actin Organization. Handb Exp Pharmacolin press.

Leithner, A., Eichner, A., Müller, J., Reversat, A., Brown, M., Schwarz, J., Merrin, J., de Gorter, D., Schur, F., Bayerl, J., de Vries, I., Wieser, S., Hauschild, R., Lai, F.P., Moser, M., Kerjaschki, D., Rottner, K., Small, J.V., Stradal, T.E., Sixt, M. (2016) Diversified actin protrusions promote environmental exploration but are dispensable for locomotion of leukocytes. Nat Cell Biol, in press.  

Andritschke, D., Dilling, S., Emmenlauer, M., Welz, T., Schmich, F., Misselwitz, B., Rämö, P., Rottner, K., Kerkhoff, E., Wada, T., Penninger, J.M., Beerenwinkel, N., Horvath, P., Dehio, C., Hardt, W.-D. (2016) A genome-wide siRNA screen implicates Spire1/2 in SipA-driven Salmonella Typhimurium host cell invasion. PLoS ONE, 11(09):e0161965.

García Ponce, A., Citalán Madrid, A.F., Vargas Robles, H., Chánez Paredes, S., Nava, P., Betanzos, A., Zarbock, A., Rottner, K., Vestweber, D., Schnoor, M. (2016) Loss of cortactin causes endothelial barrier dysfunction via disturbed adrenomedullin secretion and actomyosin contractility. Sci Rep6:29003.

Rottner, K. (2016) Formin‘ filaments at a faster CLIP. Science352(6288):894-5.

Wollscheid, H.P., Biancospino, M., He, F., Magistrati, E., Molteni, E., Lupia, M., Soffientini, P., Rottner, K., Cavallaro, U., Pozzoli, U., Mapelli, M., Walters, K.J., Polo, S. (2016) Diverse functions of myosin VI elucidated by an isoform-specific α-helix domain. Nat Struct Mol Biol23(4):300-8.

Rottner, K., Stradal, T.E.B. (2016) How distinct Arp2/3 complex variants regulate actin filament assembly. Nat Cell Biol, 18 (1):1-3.

Kühn, S., Erdmann, C., Kage, F., Block, J., Schwenkmezger, L., Steffen, A., Rottner, K., Geyer M. (2015) The structure of FMNL2-Cdc42 yields insights into the mechanism of lamellipodia and filopodia formation. Nat Commun, 6:7088.

Chazeau, A., Mehidi, A., Nair, D., Gautier, J., Leduc, C., Chamma, I., Kage, F., Kechkar, A., Thoumine, O., Rottner, K., Choquet, D., Gautreau, A., Sibarita, J.-B., Giannone, G. (2014) Nanoscale segregation of actin nucleation and elongation factors determines dendritic spine protrusion. EMBO J, 33(23):2745-64.

May, M., Schelle, I., Brakebusch, C., Rottner, K., Genth H. (2014) Rac1-dependent recruitment of PAK2 to G2 phase centrosomes and their roles in the regulation of mitotic entry. Cell Cycle13(14):2210-20.

Steffen, A., Koestler, S.A., Rottner, K. (2014) Requirements for and Consequences of Rac-dependent Protrusion. Eur J Cell Biol93(5-6):184-93.

Biosse-Duplan, M., Zalli, D., Stephens, S., Zenger, S, Neff, L., Holleboom, J.M., Lai, F.P.L., Horne, W., Rottner, K., Baron, R. (2014) Microtubule Dynamic Instability Controls Podosome Patterning in Osteoclasts through EB1, Cortactin and Src. Mol Cell Biol, 34(1):16-29.

Dang, I., Gorelik, R., Sousa, C., Linkner, J., Guérin, C., Nemethova, M., Chipysheva, T., Ermilova, V., Vacher, S., Herrada, I., Campanacci, V., Derivery, E., Steffen, A., Lakisic, G., Rottner, K., Cherfils, J., Zinn-Jutsin, S., Bièche, I., Alexandrova, A., Small, J.V., Blanchoin, L., Faix, J., Gautreau, A. (2013) Inhibitory signalling to the Arp2/3 complex steers cell migration, Nature503(7475):281-4.

Koestler, S.A., Steffen, A., Nemethova, M., Winterhoff, M., Luo, N., Holleboom, J.M., Krupp, J., Jacob, S., Vinzenz, M., Schur, F., Schlüter, K., Gunning, P.W., Winkler, C., Schmeiser, C., Faix, J., Stradal, T.E.B., Small, J.V., Rottner, K. (2013) Arp2/3 complex is essential for actin network treadmilling as well as for targeting of capping protein and cofilin. Mol Biol Cell24(18):2861-75.

Steffen, A., Ladwein, M., Dimchev, G.A., Hein, A., Schwenkmezger, L., Arens, S., Ladwein, K.I., Holleboom, J.M., Schur, F., Small, J.V., Schwarz, J., Gerhard, R., Faix, J., Stradal, T.E.B., Brakebusch, C., Rottner, K. (2013) Rac function is critical for cell migration but not required for spreading and focal adhesion formation. J Cell Sci126(20):4572-88

Junemann, A., Winterhoff, M., Nordholz, B., Rottner, K., Eichinger, L., Gräf, R., Faix, J. (2013) ForC lacks canonical formin activity but bundles actin filaments and is required and multicellular development of Dictyostelium cells. Eur J Cell Biol92(6-7):201-12. 

Wolters, M., Boyle, E.C., Lardong, K., Truelzsch, K., Steffen, A., Rottner, K., Ruckdeschel, K., Aepfelbacher, M. (2013) Cytotoxic necrotizing factor-Y boosts Yersinia effector translocation by activating Rac. J Biol Chem288(32):23543-53. 

Huelsenbeck, S.C., Roggenkamp, D., May, M., Huelsenbeck, J., Brakebusch, C., Rottner, K., Ladwein, M., Just, I., Fritz, G., Schmidt, G., Genth, H. (2013) Expression and cytoprotective activity of the small GTPase RhoB induced by the Escherichia coli cytotoxic necrotizing factor 1. Int J Biochem Cell Biol45(8):1767-75.

Vestweber, D., Zeuschner, D., Rottner, K., Schnoor, M. (2013) Cortactin regulates the activity of small GTPases and ICAM-1 clustering in endothelium: implications for the formation of docking structures. Tissue Barriers1(1): e23862. 

Thoss, F., Dietrich, F., Punkt, K., Illenberger, S., Rottner, K., Himmel, M., Ziegler, W.H. (2013) Metavinculin: New Insights into Functional Properties of a Muscle Adhesion Protein. Biochem Biophys Res Commun430(1):7-13.

Schwarz, J., Proff, J., Hävemeier, A., Ladwein, M., Rottner, K., Barlag, B., Pich, A., Tatge, H., Just, I., Gerhard, R. (2012) Serine-71 phosphorylation of Rac1 modulates downstream signaling. PLoS ONE7(9):e44358.

Vinzenz, M., Nemethova, M., Schur, F., Mueller, J., Narita, A., Urban, E., Winkler, C., Schmeiser, C., Köstler, S.A., Rottner, K., Resch, G.P., Maeda, Y., Small, J.V. (2012) Actin branching in the initiation and maintenance of lamellipodia. J Cell Sci125(11): 2775-85.

Block, J., Breitsprecher, D., Kühn, S., Winterhoff, M., Kage, F., Geffers, R., Duwe, P., Rohn, J.L., Baum, B., Brakebusch, C., Geyer, M., Stradal, T.E.B., Faix, J., Rottner, K. (2012) FMNL2 drives actin-based protrusion and migration downstream of Cdc42. Curr Biol22(11):1005-12.

Hänisch, J., Stradal, T.E.B., Rottner, K. (2012) A novel contractility pathway operating in Salmonella invasion. Virulence, 3(1):81-6.

Rottner, K., Stradal, T.E.B. (2011) Actin Dynamics and Turnover in Cell Motility. Curr Opin Cell Biol23(5):569-78.

Schnoor, M., Lai, F.P.L., Zarbock, A., Kläver, R., Polaschegg, C., Schulte, D., Weich, H.A., Oelkers, J.M., Rottner, K., Vestweber, D. (2011) Cortactin deficiency is associated with reduced neutrophil recruitment but increased vascular permeability in vivo. J Exp Med208(8):1721-35.

Breitsprecher, D., Koestler, S.A., Chizhov, I., Nemethova, M., Mueller, J., Goode, B.L., Small, J.V., Rottner, K., Faix, J. (2011) Cofilin cooperates with fascin to disassemble filopodial actin filaments. J Cell Sci124(19):3305-18.

Oelkers, J.M., Vinzenz, M., Nemethova, M., Jacob, S., Lai, F.P.L., Block, J., Szczodrak, M., Kerkhoff, E., Backert, S., Schlüter, K., Stradal, T.E.B., Small, J.V., Koestler, S.A., Rottner, K. (2011) Microtubules as platforms for assaying actin polymerization in vivo. PLoS ONE6(5):e19931.

Dubielecka, P.M., Ladwein, K.I., Xiong, X., Migeotte, I., Chorzalska, A., Anderson, K.V., Sawicki, J.A., Rottner, K., Stradal, T.E.B., Kotula, L. (2011) Essential role for Abi1 in embryonic survival and WAVE2 complex integrity. Proc Natl Acad Sci USA108(17):7022-7.

Hänisch, J., Kölm, R., Wozniczka, M., Bumann, D., Rottner, K., Stradal, T.E.B. (2011) Activation of a RhoA/Myosin II-dependent but Arp2/3-complex-independent pathway facilitates Salmonella invasion. Cell Host & Microbe9(4):273-85.

Jackson, B., Peyrollier, K., Pedersen, E., Basse, A., Karlsson, R., Wang, Z., Lefever, T., Ochsenbein, A., Schmidt, G., Aktories, A., Stanley, A:, Quondamatteo, F., Ladwein, M., Rottner, K., van Hengel, J., Brakebusch, C. (2011) RhoA is dispensable for skin development, but crucial for contraction and directed migration of keratinocytes. Mol Biol Cell22(5):593-605.

Rottner K., Hänisch J., Campellone K.G. (2010) WASH, WHAMM and JMY: regulation of Arp2/3 complex and beyond. Trends Cell Biol20(11):650-61.

Marg, S., Winkler, U., Sestu, M., Himmel, M., Schönherr, M., Bär, J., Mann, A., Moser, M., Mierke, C., Rottner, K., Blessing, M., Hirrlinger, J., Ziegler, W.H. (2010) The Vinculin-In20/21 Mouse: Characteristics of a Constitutive, Actin-Binding Deficient Splice Variant of Vinculin. PLoS One5(7):e11530.

Hertzog, M., Milanesi, F., Hazelwood, L., Disanza, A., Liu, H.J., Perlade, E., Malabarba, M.G., Pasqualato, S., Maiolica, A., Confalonieri, S., Le Clainche, C., Offenhauser, N., Block, J., Rottner, K., Di Fiore, P.P., Carlier, M.F., Volkmann, N., Hanein, D., Scita, G. (2010) Molecular basis for the dual function of Eps8 on actin dynamics: bundling and capping. PLOS Biol, 8(6):e1000387.

Klink, B.U., Barden, S., Heidler, T.V., Borchers, C., Ladwein, M., Stradal, T.E.B., Rottner, K., Heinz, D.W. (2010) Structure of Shigella IpgB2 in complex with human RhoA: Implications for the mechanism of bacterial guanine nucleotide exchange factor mimicry. J Biol Chem285(22):17197-208.

Hänisch, J., Ehinger, J., Ladwein, M., Rohde, M., Derivery, E., Bosse, T., Steffen, A., Bumann, D., Misselwitz, B., Hardt, W.-D., Gautreau, A., Stradal, T.E.B., Rottner, K. (2010) Molecular dissection of Salmonella-induced membrane ruffling versus invasion. Cell Microbiol, 12(1):84-98.

Rottner, K., Stradal, T.E.B. (2009) Poxviruses Taking a Ride on Actin: New Users of Known Hardware. Cell Host & Microbe6(6):697-9.

Lai, F.P.L., Szczodrak, M., Oelkers, J.M., Ladwein, M., Acconcia, F., Benesch, S., Auinger, S., Faix, J., Small, J.V., Polo, S., Stradal, T.E.B., Rottner, K. (2009) Cortactin promotes migration and platelet-derived growth factor-induced actin reorganization by signaling to Rho-GTPases. Mol Biol Cell20(14):3209-23.

Faix, J., Breitsprecher, D., Stradal, T.E.B., Rottner, K. (2009) Filopodia: complex models for simple rods. Int J Biochem Cell Biol41(8-9):1656-64.

Himmel, M., Ritter, A., Rothemund, S., Pauling, B.V., Rottner, K., Gingras, A.R., Ziegler, W.H. (2009) Control of high affinity interactions in the talin C-terminus – how talin domains coordinate protein dynamics in cell adhesions. J Biol Chem284(20):13832-42.

Weiss, S.M., Ladwein, M., Schmidt, D., Ehinger, J., Lommel, S., Städing, K., Beutling, U., Disanza, A., Frank, R., Jänsch, L., Scita, G., Gunzer, F., Rottner, K., Stradal, T.E.B. (2009) IRSp53 links the enterohemorrhagic E. coli effectors Tir and EspFU for actin pedestal formation. Cell Host & Microbe5(3):244-58.

Koestler, S.A., Rottner, K., Lai, F., Block, J., Vinzenz, M., Small, J.V. (2009) F- and G-actin concentrations in lamellipodia of moving cells. PLoS One4(3):e4810.

Halabi-Cabezon, I., Huelsenbeck, J., May, M., Ladwein, M., Rottner, K., Just, I., Genth, H. (2008) Prevention of the cytopathic effect induced by Clostridium difficile Toxin B by active Rac1 FEBS Lett582(27):3751-6.

Steffen, A., Le Dez, G., Poincloux, R. Recchi, C., Nassoy, P., Rottner, K., Galli, T., Chavrier, P. (2008) MT1-MMP-dependent invasion is regulated by TI-VAMP/VAMP7. Curr Biol18(12):926-31.

Block, J., Stradal, T.E.B., Haenisch, J., Geffers, R., Koestler, S.A., Urban, E., Small, J.V., Rottner, K., Faix, J. (2008) Filopodia formation induced by active Dia2/Drf3. J Microsc231(3):506-17.

Ladwein, M., Rottner, K. (2008) On the Rho’d: The regulation of membrane protrusions by Rho-GTPases. FEBS Lett582(14):2066-74.

Lai, F.P.L., Szczodrak, M., Block, J., Faix, J., Breitsprecher, D., Mannherz, H.G., Stradal, T.E.B., Dunn, G.A., Small, J.V., Rottner, K. (2008) Arp2/3-complex interactions and actin network turnover in lamellipodia. EMBO J27(7):982-92.

Koestler, S.A., Auinger, S., Vinzenz, M., Rottner, K., Small, J.V. (2008) Differentially oriented populations of actin filaments generated in lamellipodia collaborate in pushing and pausing at the cell front. Nat Cell Biol10(3):306-13.

Bosse, T., Ehinger, J., Czuchra, A., Benesch, S., Steffen, A., Wu, X., Schloen, K., Niemann, H.H., Scita, G., Stradal, T.E.B., Brakebusch, C., Rottner, K. (2007) Cdc42 and phosphoinositide 3-kinase drive Rac-mediated actin polymerization downstream of c-Met in distinct and common pathways. Mol Cell Biol27(19):6615-28.

Müller-Taubenberger, A., Vos, M.J., Böttger, A., Lasi, M., Lai, F.P.L., Fischer, M., Rottner, K. (2006) Monomeric red fluorescent protein variants used for imaging studies in different species. Europ J Cell Biol85(9-10):1119-29.

Caspani, E.M., Echevarria, D., Rottner, K., Small, J.V. (2006) Live imaging of glioblastoma cells in brain tissue shows requirement of actin bundles for migration. Neuron Glia Biol2(2):105-14.

Steffen, A., Faix, J., Resch, G.P., Linkner, J., Wehland, J., Small, J.V., Rottner, K., Stradal, T.E.B. (2006) Filopodia formation in the absence of functional WAVE- and Arp2/3-complex. Mol Biol Cell17(6):2581-91.

Faix, J., Rottner, K. (2006) The making of filopodia. Curr Opin Cell Biol, 18(1):18-25.

Stamm, L., Pak, M.A., Morisaki, J.H., Snapper, S.B., Rottner, K., Lommel, S., Brown, E.J. (2005) Role of the WASP family proteins for Mycobacterium marinum actin tail formation. Proc Natl Acad Sci USA102(41):14837-42.

Innocenti, M., Gerboth, S., Rottner, K., Lai, F.P.L., Hertzog, M., Stradal, T.E.B., Frittoli, E., Didry, D., Polo, S., Disanza, A., Benesch, S., Di Fiore, P.P., Carlier, M.F., Scita, G. (2005) Abi1 regulates the activity of N-WASP and WAVE in distinct actin-based processes. Nat Cell Biol, 7(10):969-76.

Czuchra, A., Wu, X., Meyer, H., Van Hengel, J., Schroeder, T., Geffers, R., Rottner, K., Brakebusch, C. (2005) Cdc42 is not essential for filopodium formation, directed migration, cell polarization and mitosis in fibroblastoid cells. Mol Biol Cell16(10):4473-84.

Rottner, K., Stradal, T.E.B., Wehland, J. (2005) Bacteria – host-cell interactions at the 
plasma membrane: stories on actin cytoskeleton subversion. Dev Cell9:3-17.

Benesch, S., Polo, S., Lai, F.P.L., Anderson, K.I., Stradal, T.E.B., Wehland, J., Rottner, K. (2005) N-WASP deficiency impairs EGF internalization and actin assembly at clathrin coated pits. J Cell Sci, 118(14):3103-15.

Disanza, A., Steffen, A., Hertzog, M., Frittoli, E., Rottner, K., Scita, G. (2005) Actin polymerization machinery: the finish line of signaling networks, the starting point of cellular movement. Cell Mol Life Sci62(9):955-70.

Rottner, K., Lommel, S., Wehland, J., Stradal, T.E.B. (2004) Pathogen-induced actin filament rearrangement in infectious diseases. J Pathol204(4):396-406.

Pacholsky, D., Vakeel, P., Himmel, M., Löwe, T., Stradal, T.E.B., Rottner, K., Fürst, D.O., van der Ven, P.F. (2004) Xin repeats define a novel actin-binding motif. J Cell Sci117:5257-68.

Stradal, T.E.B., Rottner, K., Disanza, A., Confalonieri, S., Innocenti, M., Scita, G. (2004) Regulation of actin dynamics by WASP and WAVE family proteins. Trends Cell Biol14(6):303-11.

Lommel, S., Benesch, S., Rohde, M., Wehland, J., Rottner, K. (2004) 
Enterohaemorrhagic and enteropathogenic Escherichia coli use different mechanisms for actin pedestal formation that converge on N-WASP. Cell Microbiol6(3):243-54.

Steffen, A., Rottner, K., Ehinger, J., Innocenti, M., Scita, G., Wehland J., Stradal, T.E.B. (2004) Sra-1 and Nap1 link Rac to actin assembly driving lamellipodia formation. EMBO J23(4):749-59.

Breitbach, K., Rottner, K., Klocke, S., Rohde, M., Jenzora, A., Wehland, J., Steinmetz, I. (2003) Actin-based motility of Burkholderia pseudomallei involves the Arp2/3 complex, but not N-WASP and Ena/VASP proteins. Cell Microbiol5(6):385-93.

Benesch, S., Lommel, S., Steffen, A., Stradal, T.E.B., Scaplehorn, N., Way, M., Wehland, J., Rottner, K. (2002) Phosphatidylinositol 4,5-biphosphate (PIP2)-induced vesicle movement depends on N-WASP and involves Nck, WIP, and Grb2. J Biol Chem277:37771-6.

Small, J.V., Stradal, T., Vignal, E., Rottner, K. (2002) The lamellipodium: where motility begins. Trends Cell Biol12(3):112-20.

Rottner, K., Krause, M., Gimona, M., Small, J.V., Wehland, J. (2001) Zyxin is not colocalized with vasodilator-stimulated phosphoprotein (VASP) at lamellipodial tips and exhibits different dynamics to vinculin, paxillin, and VASP in focal adhesions. Mol Biol Cell12(10):3103-13.

Lommel, S., Benesch, S., Rottner, K., Franz, T., Wehland, J., Kühn, R. (2001) Actin pedestal formation by enteropathogenic Escherichia coli and intracellular motility of Shigella flexneri are abolished in N-WASP-defective cells. EMBO Rep2(9):850-7.

Stradal, T.E.B., Courtney, K.D., Rottner, K., Hahne, P., Small, J.V., Pendergast, A.M. (2001) The Abl interactor proteins localize to sites of actin polymerization at the tips of lamellipodia and filopodia. Curr Biol11(11):891-5.

Kohler, A., Jostarndt-Fögen, K., Rottner, K., Alliegro, M.C., Draeger, A. (1999) Intima-like smooth muscle.cells: developmental link between endothelium and media? Anat Embryol (Berl)200(3):313-23.

Small, J.V., Rottner, K., Hahne, P., Anderson, K.I. (1999) Visualising the actin cytoskeleton. Microsc Res Tech47(1):3-17.

Rottner, K., Behrendt, B., Small, J.V., Wehland, J. (1999) VASP dynamics during lamellipodia protrusion. Nat Cell Biol1(5):321-2.

Small, J.V., Kaverina, I., Krylyshkina, O., Rottner, K. (1999) Cytoskeleton cross-talk during cell motility. FEBS Lett452(1-2):96-9.

Rottner, K., Hall, A., Small, J.V. (1999) Interplay between Rac and Rho in the control of substrate contact dynamics. Curr Biol9(12):640-8.

Small, J.V., Rottner, K., Kaverina, I. (1999) Functional design in the actin cytoskeleton. Curr Opin Cell Biol11(1):54-60.

Small, J.V., Rottner, K., Kaverina, I., Anderson, K.I. (1998) Assembling an actin cytoskeleton for cell attachment and movement. Biochim Biophys Acta1404(3):271-81.

Kaverina, I., Rottner, K., Small, J.V. (1998) Targeting, capture, and stabilization of microtubules at early focal adhesions. J Cell Biol142(1):181-90.

Small, J.V., Anderson, K., Rottner, K. (1996) Actin and the coordination of protrusion, attachment and retraction in cell crawling. Biosci Rep16(5):351-68.

Bernroider, G., Holztrattner, M., Rottner, K. (1996) Sex steroid-opioid interactions associated with the temporal component of avian calling patterns. Horm Behav30(4):583-9.

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