Microbial Diagnostics

Microbial pathogens find their way into our bodies in different of ways. Two important routes of transmission include food and drinking water. If a food-borne pathogen triggers an epidemic, the starting point of the chain of infection must quickly be identified to effectively curtail the epidemic. Now, HZI scientists have found a way to trace the routes of infection of bacterial pathogens that are present in our food and drinking water. Using high-resolution methods, the researchers are able to readily identify individual pathogenic bacterial strains. 

Leader

Team

Prof Dr Manfred Höfle

Head of the Research Group Microbial Diagnostics

Publications

2016

Lesnik R, Brettar I, Höfle MG (2016) Legionella species diversity and dynamics from surface reservoir to tap water: from cold adaptation to thermophily. ISME-J doi:10.1038/ismej.2015.199  

Vezzulli L, Grande C, Reid PC, Hélaouët P, Edwards M,  Höfle MG,  Brettar I,  Colwell RR,  Pruzzo C (2016) Climate influence on Vibrio and associated human diseases during the past half-century in the coastal North Atlantic. PNAS E5062–E5071, Published online August 8, 2016, www.pnas.org/cgi/doi/10.1073/pnas.1609157113 

Brettar I, Höfle MG, Pruzzo, C, Vezzulli, L (2016). Climate change effects on planktonic bacterial communities in the ocean - from structure and function to long-term and large scale observations.In J. Marxsen (ed.) "Microbial Ecology and Climate Change: Current - Research and Future Trends", Horizon Scientific Press, Poole, UK.

Seidel M, Jurzik L,  Brettar I,  Höfle MG, Griebler C (2016). Microbial and viral pathogens in freshwater: current research aspects studied in Germany. Environ Earth Sci 75:1384, DOI 10.1007/s12665-016-6189-x  

Vezzulli L., Grande C, Tassistro G,  Brettar I, Höfle MG, Pereira R, Mushi  D,  Pallavicini A, Vassallo P,  Pruzzo C (2016) Whole-genome enrichment provides deep insights into Vibrio cholerae metagenome from an African river. Microbial Ecology, DOI 10.1007/s00248-016-0902-x

Mizrahi H, Peretz A,  Lesnik R, Aizenberg-Gershtein Y, Rodriguez-Martinez S,  Sharaby Y, Pastukh N, Brettar I, Höfle MG, Halpern M (2016) Comparison of sputum microbiome of legionellosis-associated patients and other pneumonia patients: indications for polybacterial infections. Scientific Reports, 6, 40114, DOI 10.1038/srep40114

2015

Vezzulli L,  Pezzati E, Brettar I,  Höfle MG, Pruzzo C (2015). Effects of Global Warming on Vibrio Ecology. Microbiol Spectrum 3(2):VE-0004-2014. doi:10.1128/microbiolspec.VE-0004-2014 

Kramer R, Sauer-Heilborn A, Welte T, Jauregui R, Brettar I, Guzman CA,  Höfle MG. (2015). High Individuality of Respiratory Bacterial Communities in a Large Cohort of Adult Cystic Fibrosis Patients under Continuous Antibiotic Treatment. Plos One 10(2): e0117436, doi:10.137/journal.pone. 0117436 

Rodríguez-Martínez S, Sharaby Y, Pecellín M, Brettar I, Höfle MG, Halpern M (2015) Spatial distribution of Legionella pneumophila MLVA-genotypes in a drinking water system. Water Research 77, 119-132 

2014

Deng J, Brettar I,  Luo C,  Auchtung J, Konstantinidis KT, Rodrigues JLM,  Höfle MG, Tiedje JM (2014). Stability, Genotypic and Phenotypic Diversity of Shewanella baltica in the Redox Transition Zone of the Baltic Sea. Environ. Microbiol. 16: 1854-1866. DOI: 10.1111/1462-2920.12344

2013

Henne, K., L. Kahlisch, M. G. Höfle and I. Brettar (2013) 
Seasonal dynamics of bacterial community structure and composition in cold and hot drinking water derived from surface water reservoirs. Water Research,
http://dx.doi.org/10.1016/j.watres.2013.06.034
 
Deng, J., Brettar, I., Auchtung, J., Konstantinidis, K.T., Rodriguez, J.L.M, Höfle,  M.G. and Tiedje, J.M. (2014)
Stability, Genotypic and Phenotypic Diversity of Shewanella baltica in the Redox Transition Zone of the Baltic Sea. Environmental Microbiol. doi:10.1111/1462-2920.12344 

2012

Brettar, I., R. Christen, and M.G. Höfle. (2012). Analysis of bacterial core communities in the central Baltic by comparative RNA-DNA-based fingerprinting provides links to structure-function relationships. ISME J. 6: 195-212 http://www.ncbi.nlm.nih.gov/pubmed/21697960

Vezzulli, L,  Brettar I, Pezzati E, , Reid PC, Colwell R.R., Höfle MG, and Pruzzo, C (2012) Long-term effects of ocean warming on the prokaryotic community: evidence from the vibrios. ISME J. 6:21-30 doi:10.1038/ismej.2011.89

Kahlisch, L. K. Henne, L. Gröbe, I. Brettar and M.G. Höfle (2012) Assessing the species composition of viable bacteria in drinking water using Fluorescence Activated Cell Sorting (FACS) and community fingerprinting. Microbial Ecol. 63:383-397 doi: 10.1007/s00248-011-9918-4

Caro-Quintero, A. J. Auchtung , J. Deng,  I. Brettar, M.G. Höfle,  J. M. Tiedje and K. T Konstantinidis (2012). Genome sequencing of five Shewanella baltica strains recovered from the oxic-anoxic interface of the Baltic Sea. J. Bacteriol. 194:1236

Henne, K., Kahlisch, L., Brettar, I., and Höfle, M. G. (2012). Analysis of structure and composition of bacterial core communities in mature drinking water biofilms and bulk water of a citywide network in Germany. Appl. Environ. Microbiol. 78: 3530-3538 http://www.ncbi.nlm.nih.gov/pubmed/22389373

2011

Caro-Quintero, A. J. Deng,  J. Auchtung, I. Brettar, M.G. Höfle and K. T Konstantinidis (2011). Unprecedented levels of horizontal gene transfer among spatially co-occurring Shewanella bacteria from the Baltic Sea. ISME J 5:131-140 10.1038/ismej.2010.93

Brettar, I., R. Christen, and M.G. Höfle. (2011). "Genus III. Aquiflexum Brettar, Christen and Höfle 2004b, 2339VP " in Bergey's Manual of Systematic Bacteriology, 2nd edition, Volume 4, The Bacteroidetes, Spirochaetae, Tenericutes (Molicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrumicrobia, Chlamydiae, and Planctomycetes. Edited by N. R. Krieg et al., p. 433-434. 

Brettar, I., R. Christen, and M.G. Höfle. (2011). "Genus IV. Belliella Brettar, Christen and Höfle 2004a, 69VP " in Bergey's Manual of Systematic Bacteriology, 2nd edition, Volume 4, The Bacteroidetes, Spirochaetae, Tenericutes (Molicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrumicrobia, Chlamydiae, and Planctomycetes. Edited by N. R. Krieg et al., p. 434-437.

2010

Kahlisch, L. K. Henne, L. Groebe, J. Draheim, M.G. Höfle and I. Brettar (2010) Molecular analysis of the bacterial drinking water community with respect to live/dead status. Water Science & Technology-WST 61.1:9-14 http://www.ncbi.nlm.nih.gov/pubmed/20057086

Kahlisch, L. K. Henne, J. Draheim, I. Brettar and M.G. Höfle (2010). High-resolution in situ genotyping of Legionella pneumophila populations in drinking water by Multiple-Locus Variable-Number of Tandem Repeat Analysis (MLVA) using environmental DNA. Appl. Environ. Microbiol. 76:6186-6195

2009

Harth-Chu, E. Especio R. T., Christen, R., Guzman, C.A., and M.G. Höfle (2009)Multiple-locus variable-number of tandem-repeats analysis for clonal identification of  Vibrio parahaemolyticus isolates using capillary electrophoresis. Appl. Environ. Microbiol. 75: 4079-4088

2008

Brettar, I. and M.G. Höfle. (2008). Molecular assessment of bacterial pathogens – a contribution to drinking water safety. Curr. Op. Biotech. 19:274-280

Höfle, M.G.,  Kirchman, D. L., Christen, R. and I. Brettar (2008) Molecular diversity of bacterioplankton: link to a predictive biogeochemistry of pelagic ecosystems. Aquatic Microbiol. Ecol. 53:39-58

Henne, K., L. Kahlisch, J. Draheim, I. Brettar, and M.G. Höfle (2008) Polyvalent fingerprint based molecular surveillance methods for drinking water supply systems. Water Science & Technology: Water Supply – WSTWS 8:531-536  

2007

Gattinger,A., Höfle, M. G., M. Schloter, A. Embacher, F. Böhme, J. C. Munch and M. Labrenz (2007) Traditional cattle manure application determines abundance, diversity and activity of methanogenic Archaea in an arable European soil. Environ. Microbiol. 9:612-624.

Brettar, I., C. A. Guzman, and M.G. Höfle. (2007).  Human pathogenic micro-organisms in the marine environment - an ecological perspective. CIESM Reports No. 31,  Marine Sciences and public health - some major issues. Geneva, September 2006, p. 59-68. (http://www.ciesm.org/online/monographs/Geneva.html)

Brettar, I., Christen, R., Bötel, J., Lünsdorf, H. and M. G. Höfle. (2007) Anderseniella baltica gen. nov. sp. nov. a novel marine bacterium of the Alphaproteo-bacteria isolated from sediment in the central Baltic Sea. Int. J. Syst. Evol. Microbiol. 75: 2399-2405 

2006

González-Escalona, N., Fey, A., Höfle, M. G., Espejo, R. T. and C. A. Guzmán (2006) Quantitative reverse transcription polymerase chain reaction analysis of Vibrio cholerae cells entering the viable but non-culturable state and starvation in response to cold shock. Environ. Microbiol. 8: 658-666.

Brettar, I., Labrenz, M., Flavier, S, Bötel, J., Kuosa, H., Christen, R. and M. G. Höfle. (2006) Identification of a Thiomicrospira denitrificans-like epsilonbacterium as a catalyst for denitrification in the central Baltic sea. Appl. Environ. Microbiol., 72: 1364-1372.

Eichler, S. Christen, R., Höltje, C. Westphal, P., Bötel, J., Brettar, I. Mehling, A. and M. G.   Höfle. (2006) Composition and dynamics of bacterial communities of a drinking water supply system as assessed by RNA- and DNA-based 16S rRNA gene fingerprinting. Appl. Environ. Microbiol. 72:1858-1872. 

Weinbauer, M. G., Christen R. and M. G. Höfle. (2006) The response of Vibrio and Rhodobacter-related populations of the NW Mediterranean Sea to additions of dissolved organic matter, phages, or dilution. Microbial Ecol. 51: 336-344.

Brettar, I. Christen, R. and M. G. Höfle (2006) Rheinheimera perlucida sp. nov.,  a novel marine bacterium of the Gammaproteobacteriaisolated from surface water of the central Baltic Sea. Int. J. Syst. Evol. Microbiol. 56:2177-2183

2005

Höfle, M. G., Flavier, R. Christen, J. Bötel, M. Labrenz and I. Brettar (2005) Retrieval of nearly complete 16S rRNA gene sequences from environmental DNA following 16S rRNA-based community fingerprinting. Environ. Microbiol. 7: 670-675.

2004

Brettar, I. Christen, R. and M. G. Höfle (2004) Belliella baltica gen. nov., sp. nov., a novel marine bacterium of the Cytophaga-Flavobacterium-Bacteroides group isolated from surface water of the central Baltic Sea. Int. J. Syst. Evol. Microbiol, 54: 65-70.

Fey, A., Eichler, S., Flavier, S., Christen, R., Höfle, M. G.  and Guzmán, C. A. (2004) Development of a real-time PCR-based approach for accurate quantification of bacterial RNA targets in water, using Salmonella as a model organism. Appl. Environ. Microbiol., 70: 3618-3623.

Labrenz M., I. Brettar, S. Flavier, R. Christen, J. Bötel and Höfle, M. G. (2004) Development and application of a real-time PCR approach for quantification of  uncultured bacteria in the central Baltic Sea. Appl. Environ. Microbiol.,70: 4971-4979.

Brettar, I. Christen, R. and M. G. Höfle (2004) Aquiflexum balticum gen. nov., sp. nov., a novel marine bacterium of the Cytophaga-     Flavobacterium-Bacteroides group isolated from surface water of the central Baltic Sea.      Int. J. Syst. Evol. Microbiol., 54: 2335-2341.

Höfle, M. G. (2004) Genotyping of bacterial isolates from the environment using low-molecular-weight RNA fingerprints. In: Kowalchuk, G. A., Bruijn, F.J.d., Head, I. M., Akkermans, A. D. L., and Elsas, J. D. v. (eds),  Molecular Microbial Ecology Manual, Second edition Kluwer Academic Publishers, Dordrecht, the Netherlands, 3.05: 587-610.

70 Eichler, S., Weinbauer, M. G., Dominik, D. and M. G. Höfle (2004) Extraction of total RNA and DNA from bacterioplankton. In: Kowalchuk, G. A., Bruijn, F.J.d., Head, I. M., Akkermans, A. D. L., and Elsas, J. D. v. (eds),  Molecular Microbial Ecology Manual, Second edition, Kluwer Academic Publishers, Dordrecht, the Netherlands, 1.0.8: 103-120.

2003

Hahn, M.W., Lünsdorf, H., Lu, Q., Schauer, M., Höfle, M. G., Boenigk, and P. Stadler. (2003) Isolation of novel ultramicrobacteria belonging to the Actinobacteria from five freshwater habitats in Europe and Asia. Appl. Environ. Microbiol. 69: 1442-1451

Brettar, I. Christen, R. and M. G. Höfle.(2003) Idiomarina baltica, sp. nov., a marine bacterium with high temperature optimum isolated from surface water of the Central Baltic Sea. Int. J. Syst. Evol. Microbiol., 53: 407-413

Lucas, F., Bertru, G., and M. G. Höfle (2003) Characterization of free-living and attached bacteria in sediments colonized by Hediste (Nereis) diversicolor. Aquatic Microbial Ecology 32:165-174

Weinbauer, M. G, Brettar I. and M. G. Höfle. (2003) Viral infection and lysogeny of bacterioplankton in surface, deep and anoxic marine waters. Limnol. Oceanogr. 48: 1457-1465

2002

Weinbauer, M. G, Fritz, I., Wenderoth D. F., and M. G. Höfle. (2002) Simultaneous extraction of total RNA and DNA from bacterioplankton suitable for quantitative structure and function analyses. Appl. Environ. Microbiol. 68:1082-1087 

Weinbauer, M. G, Winter C., and M. G. Höfle. (2002) Reconsidering transmission electron microscopy based estimates of viral infection of bacterioplankton using conversion factors derived from natural communities, Aquat. Microb. Ecol. 27:103-110 

Brettar, I. and M. G. Höfle. (2002) Close correlation between the nitrate elimination rate by denitrification and the organic matter content in hardwood forest soils of the upper Rhine floodplain (France). Wetlands 22:214-224

Dominik, K. and M.G. Höfle. (2002) Changes in bacterioplankton community structure and activity with depth in a eutrophic lake as revealed by 5S rRNA analysis. Appl. Environ. Microbiol. 68:3606-3613 

Brettar, I. Christen, R. and M. G. Höfle. (2002) Rheinheimera baltica gen. nov., sp. nov., a blue coloured bacterium isolated from the central Baltic sea. Int. J. Syst. Evol. Microbiol. 52: 1851-1857 

Pöhler, I., Wenderoth, D.F., Wendt-Potthoff K., and M. G. Höfle. (2002) Bacterioplankton community structure and dynamics in enclosures during bioremediation experiments in an acid mining lake. Water, air and soil pollution: Focus 2: 111-121

Brettar, I. Christen, R. and M. G. Höfle. (2002) Shewanella denitrificans, sp. nov., a vigorously denitrifying bacterium isolated from the oxic-anoxic interface of the Gotland Deep in the central Baltic Sea. Int. J. Syst. Evol. Microbiol. 52: 2211-2217

2001

Hahn, M. W. and M. G. Höfle. (2001) Grazing of protozoa and its effect on populations of aquatic bacteria. FEMS Microbiol. Ecol. 35: 113-121

Höfle, M. G. (2001) Marine Biotechnology – Summary of the Discussion Round. In: K.G. Barthel et al. (eds) Proceedings of the EurOCEAN 2000 Conference, p. 263-267. Office for publications of the European Communities, Luxemburg, ISBN 92-894-1019-1.

Brettar, I,  Moore, E.R.B. and M. G. Höfle. (2001) Phylogeny and abundance of novel denitrifying bacteria isolated from the water column of the central Baltic sea. Microb. Ecol. 42: 295-305 

Weinbauer, M. G, and M. G. Höfle. (2001) Quantification of nucleic acids from aquatic environments by using green-fluorescent dyes and microtiter plates. In: A.D.L. Akkermans, J.D. van Elsas, F.J. de Bruijn (eds.) Molecular Microbial Ecology Manual, Kluwer Academic Publishers, Dordrecht, The Netherlands. 2.1.3:1-10 

2000

Hahn, M. W., Moore, E.R.B. and M. G. Höfle. (2000). Role of microcolony formation in the protistan grazing de­fense of the aquatic bacterium Pseudomonas sp. MWH1. Microbial Ecol. 39: 175-185

Höfle, M. G., Ziemke, F., and I. Brettar, I.(2000) Niche differentiation of Shewanella putrefaciens populations from the Baltic as revealed  by molecular and metabolic fingerprinting. In: Bell, C.R. Brylinsky M., and P. Johnson-Green (eds), Microbial Biosystems: New Frontiers, Proceedings of ISME8, Halifax, Canada, p. 135-143

Höfle, M. G., Brettar, I., Engelen, B. Berry, A, Wellington, E. De la Broise, D., Sjöholm, C, (2000) Marine bacterial genes and isolates as sources for novel biotechnological products, p. 153-158. In EurOCEAN 2000 – Vol.1 Marine processes, ecosystems and interactions. Office for publications of the European Communities, Luxembourg, ISBN 92-828-9713-3 

1999

Dominik, K. and M.G. Höfle. (1999) Extraction of total RNA from bacterioplankton. In: A.D.L. Akkermans, J.D. van Elsas, F.J. de Bruijn (eds.) Molecular Microbial Ecology Manual, Kluwer Academic Publishers, Dordrecht, The Netherlands., 1.2.2.:1-9.

Hahn, M. W., Moore, E.R.B. and M. G. Höfle. (1999) Bacterial filament formation, a defense mechanism against flagellate grazing, is growth rate controlled in bacteria of different phyla. Appl. Environ. Microbiol. 65: 25-35

Höfle, M. G. (1999). Direct detection of nutrient induced changes in the community structure of bacterioplankton using low-molecular-weight RNA Analysis. Arch. Hydrobiol. Spec. Issues Advanc. Limnol. 54: 199-211

Guiliano, L., De Domenico, M. De Domenico, E., Höfle, M. G., and Yakimov, M. (1999). Identification of culturable oligotrophic bacteria within naturally occurring  bacterioplankton communities of the Ligurian Sea by 16S rRNA sequencing and probing. Microbial Ecol. 37: 77-85

Höfle, M. G., Haas, H. and K. Dominik (1999). Seasonal dynamics of  bacterioplankton community structure in a eutrophic lake as determined by 5S rRNA analysis. Appl. Environ. Microbiol. 65: 3164-3174

Hahn, M. W., and M. G. Höfle. (1999). Flagellate predation on a bacterial model community: Interplay of size-selective grazing, specific bacterial cell size, and bacterial community composition. Appl. Environ. Microbiol. 65: 4863-4872

Vybrial, D., Denner, E. B. M., Haller, C. M. , Busse, H.-J., Witte, A., Höfle, M. G., and       Velimirov, B. (1999). Polyphasic classification of 0.2 μm filterable bacteria from the Western Mediterranean Sea. Syst. and Appl. Microbiol. 22: 635-646

Höfle, M. G. (1999). Marine bacterial genes and isolates as sources for novel biotechnological products.  In: Barthel K.G. (ed) Proceedings of the third European Marine Science and Technology conference. European Commission, ISBN 92-828-3036-5, Vol 3: 1246-1252 

Höfle, M. G. and Abraham W.-R .(1999). Eighth international symposium on microbial ecology (ISME8) - microbial ecology on the doorstep of the 21st century. Environm. Microbiol. 1, 107-108

1998

Höfle, M. G. (1998) Genotyping of bacterial isolates from the environment using low-molecular-weight RNA fingerprints. In: Akkermans, A. D. L., J. D. van Elsas, and  F. J. de Bruijn (eds),  Molecular Microbial Ecology Manual, Kluwer academic publisher, Dordrecht, Netherlands, 3.3.7:1-23

Weinbauer, M. and M. G. Höfle (1998) Significance of viral lysis and flagellate grazing as controlling factors of bacterioplankton production in an eutrophic lake. Appl. Environ. Microbiol. 64: 431-438

Ziemke, F., Höfle, M. G., Lalucat J. and Rosselo-Mora R. (1998). Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica, sp. nov. Int. J. Syst. Bacteriol. 48: 179-186

Hahn, M. W. and M. G. Höfle (1998) Grazing pressure by a bacterivorous flagellate reverses the relative abundance of Comamonas acidovorans PX 54 and Vibrio strain CB5 in chemostat cocultures. Appl. Environ. Microbiol. 64: 1910-1918

Weinbauer, M. and M. G. Höfle (1998) Size specific mortality of lake bacterioplankton by natural viral communites. Aquat. Microb. Ecol. 15: 103-113

Weinbauer, M. and M.G. Höfle. (1998) Distribution and life strategies of two bacterial populations in a eutrophic lake. Appl. Environ. Microbiol. 64: 3776-3783

Weinbauer, M. G, Beckmann C. and M. G. Höfle. (1998) Utility of green fluorescent nucleic acid dyes and aluminum oxide membrane filters for rapid epifluorescence enumeration of soil and sediment bacteria. Appl. Environ. Microbiol. 64: 5000-5003

1997

Ziemke, F., Brettar, I. and M. G. Höfle (1997) Stability and diversity of the genetic structure of a Shewanella putrefaciens population in the water column of the central Baltic. Aquat. Microb. Ecol. 13: 63-74

Faude, U. and M. G. Höfle (1997) Development and application of monoclonal antibodies for in-situ detection of indigenous bacterial strains in aquatic ecosystems. Appl. Environ. Microbiol. 63: 4534-4542. 

1996

Höfle, M. G. and Brettar I. (1996) Genotyping of heterotrophic bacteria from the central Baltic Sea by use of Low-Molecular-Weight RNA profiles. Appl. Environ. Microbiol. 62: 1383-1390 

1995

Höfle, M. G. and I. Brettar. (1995) Taxonomic diversity and metabolic activity of microbial communities in the water column of the central Baltic Sea. Limnol. Oceanogr. 40: 868-874. 

Katsivela, E. and M. G. Höfle. (1995) Separation of transfer RNA and ribosomal RNA using capillary electrophoresis. J. Chromatogr. A 700: 125-136

Katsivela, E. and M. G. Höfle (1995) Low-molecular-weight RNA fingerprinting of bacteria by capillary electro phoresis. J. Chromatogr. A 717: 91-104

1994

Brettar, I., Ramos-Gonzales, M.I., Ramos, J.L. and M. G. Höfle. (1994) Fate of Pseudomonas putida after release into lake water mesocosms: survival  behaviour in response to environmental conditions. Microbial Ecology 27:  99-122.

Höfle, M. G. (1994) Structure and dynamics of aquatic microbial communities using low-molecular-weight RNA analysis. In: M. Yasuno, M. M. Watanabe (eds). Biodiversity: It's complexity and role, Global environmental forum, Tokio, p. 15-24.

1993

Brettar, I. and M.G. Höfle. (1993) Nitrous oxide producing heterotrophic bacteria from the water column of the central Baltic: abundance and molecular identification. Mar. Ecol. Progr. Ser. 94: 253-265.

Höfle, M. G. (1993) Community structure and dynamics of bacterioplankton as revealed by low-molecular- weigth RNA analysis. In: R. Guerrero, C. Pedros-Alio (eds). Trends in Microbial Ecology, Spanish Soc. Microbiol., Barcelona, p. 391-395.

Hofmann, W. and M. G. Höfle. (1993) Rotifer population dynamics in response to increased bacterial biomass and nutrients: a mesocosm experiment. Hydrobiologia 255/256: 171-175.

1992

Brettar, I. and M. G. Höfle. (1992) Influence of ecosystematic factors on survival of Escherichia coli after large-scale release into lake water mesocosms. Appl. Environ. Microbiol. 58: 2201-2210.

Höfle, M. G. (1992) Rapid genotyping of pseudomonads by using low-molecular-weight RNA profiles. In: Galli E. and Silver M. (eds), "Pseudomonas: Molecular Biology and Biotechnology", ASM Publication, Washington, p. 116-126. 

Rüger, H.J. and M.G. Höfle. (1992) Marine star‑shaped‑aggregate forming bacteriaAgrobacterium atlanticum sp. nov.;  Agrobacterium meteori sp. nov.; Agrobacterium ferrugineum sp. nov., nom. rev.; Agrobacterium gelatinovorum sp. nov., nom. rev.; and Agrobacterium stellulatum sp. nov., nom. rev. Int. J. Syst. Bacteriol. 42: 133‑143. 

Höfle, M. G. (1992) Bacterioplankton community structure and dynamics after large-scale release of nonindigenous bacteria as revealed by Low-molecular-weight RNA analysis. Appl. Environ. Microbiol. 58: 3387-3394.   

1990

Höfle, M. G. (1990) Transfer RNAs as genotypic fingerprint of eubacteria. Arch. Microbiol. 153: 299‑304. 

Höfle, M. G. (1990) RNA chemotaxonomy of bacterial isolates and natural microbial communites. In: Overbeck J. and Chrost C. (eds) "Aquatic Microbial Ecology ‑ Biochemical and Molecular Approaches", Springer‑Verlag, Berlin, p. 129‑158. 

1989

Höfle, M. G. (1989) Estimation of the taxonomic structure of natural bacterial assemblages by low molecular       RNA profiles. In Hattori T. et al. (eds) "Recent advances in microbial ecology". Japan          Scientific Societies Press, Tokyo, p. 692‑696. 

1988

Höfle, M. G., Stahl, D. A., Sayler, G., Atlas, R. M., Barry, G. F., Muyzer, G., Steffan, R. J. and Stewart‑Tull, D. E. (1988) Detection methods including sequencing and probes. In Sussman, M., Collins, C. H., Skinner, F. A. & Stewart‑Tull D. E. (eds), "The release of genetically‑engineered micro‑organisms", Academic Press, London, p. 207‑230. 

Höfle, M. G. (1988) Identification of bacteria by low molecular weight RNA profiles: a new chemotaxonomic approach. J. Microbiol. Methods 8: 235‑248. 

Höfle, M. G. (1988) Taxonomic structure of bacterial communities in mixed cultures as measured by low molecular weight RNA profiles. Arch. Hydrobiol. Beih. 31: 71‑77. 

1987

Tranvik, L.J. and M. G. Höfle.  (1987) Bacterial growth in mixed cultures on dissolved organic carbon from humic and clear waters. Appl. Environ. Microbiol. 53: 482‑488. 

1984

Höfle, M. G. (1984) Transient responses of glucose‑limited cultures of Cytophaga johnsonae to nutrient excess and starvation. Appl. Environ. Microbiol. 47: 356‑362. 

Höfle, M. G. (1984) Degradation of putrescine and cadaverine in seawater cultures by marine bacteria. Appl.  Environ. Microbiol. 47: 843‑849. 

Höfle, M. G. (1984) Can mixed cultures help the understanding of natural heterotrophic processes? Arch. Hydrobiol. Beih. 19: 53‑58. 

Höfle, M. G., C. Krambeck, J. Overbeck and K‑P. Witzel (1984). Research strategies in aquatic microbial ecology.  Arch. Hydrobiol. Beih. 19:  VII‑XII. 

1983           

Höfle, M. G. (1983) Long‑term changes in chemostat cultures of Cytophaga johnsonae. Appl. Environ. Microbiol. 46: 1045‑1053. 

1982

Höfle, M. G. (1982) Glucose uptake of Cytophaga johnsonae studied in batch and chemostat culture. Arch. Microbiol. 133: 289‑294. 

1980

Höfle, M. G. (1980) Variations of glucose uptake of Cytophaga johnsonae at different physiological states. Contin. Cultiv. Microorganisms, Proc. 7th Symposium, Prague, p. 299‑304. 

1979

Höfle, M. G. (1979) Effects of sudden temperature shifts on pure cultures of four strains of freshwater bacteria. Microbial Ecol.  5: 17‑26.

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  • Legionärskrankheit – Wissenschaftler auf Spurensuche in der Wasserleitung Legionellen warten überall auf uns wo warmes Wasser gespeichert wird – in Wasserleitungen, Klimaanlagen, Schwimmbädern. Einige wenige dieser Bakterien können schon ausreichen, um bei uns eine schwere Lungenentzündung zu verursachen. Häufen sich Fälle – etwa in einem Einkaufszentrum oder einem Schwimmbad – gehen Wissenschaftler auf Legionellensuche. Manfred Höfle hat eine Technologie entwickelt, mit der er genau die Legionellen findet, die die Menschen krank machen. Begleiten Sie ihn bei seiner Detektivarbeit...
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