Signal network analyses of the Met pathway activated by the invasin Internalin B from Listeria monocytogenes
This project aims at the characterization of the host signal network that is manipulated in the process of Listeria monocytogenes cell invasion mediated by its surface protein Internalin B (InlB). Thereby InlB, amongst others, interacts with the human Met receptor, whose original ligand is the hepatocyte growth factor (HGF), which leads to the endocytosis of the pathogen. Due to the fact that 500 human protein kinases virtually control every signalling pathway, kinase enrichment chromatography and time-resolved phosphoproteome analyses are used to reveal those kinases responding to and involved in the process of InlB stimulation. Candidates of newly found kinases are functionally analyzed with respect to Listeria invasion and physiological Met phenotypes.