Recoding Mechanisms in Infections
Many important viruses such as Ebola, Influenza, and HIV use RNA as genetic material. These viruses have an extremely small genome size compared to the eukaryotic host genomes, and therefore employ various alternative translation strategies such as stop codon read through, leaky scanning, non-IRES initiation and ribosome frameshifting to express their genes by the host translation machinery. Interestingly, the same strategies are also used in the host’s cellular gene expression. With our research we aim to understand how translational recoding changes the rules of standard decoding, allows simultaneous encoding of multiple proteins from the same mRNA and regulates gene expression in time and space. This group is located at the Helmholtz Institute for RNA-based Infection Research (HIRI).
Bock L.*, Caliskan N.*, Korniy N., Peske F., Rodnina MV. and Grubmueller H. (2019). Thermodynamic control of –1 programmed ribosomal frameshifting, Nature Communications, in press. (*co-first authors)
Matsumoto S., Caliskan N., Rodnina M. V., Murata A., and Nakatani K. (2018). Small synthetic molecule as an activator for −1 ribosomal frameshifting, Nucleic Acid Research, 46(16): 8079-8089
Caliskan N, Wohlgemuth I, Korniy N, Pearson M, Peske F, Rodnina MV Conditional Switch between Frameshifting Regimes upon Translation of dnaX mRNA Mol Cell 2017, 66(4): 558-567
Belardinelli R., Sharma H., Caliskan N., Peske F., Wintermeyer W. and Rodnina M. V. (2016). Choreography of molecular movements during ribosome progression along the mRNA. Nature Structural and Molecular Biology, 23(4):342-8
Caliskan N, Peske F, Rodnina MV Changed in translation: mRNA recoding by -1 programmed ribosomal frameshifting Trends Biochem Sci 2015, 40(5): 265-274
Caliskan N, Katunin VI, Belardinelli R, Peske F, Rodnina MV Programmed -1 frameshifting by kinetic partitioning during impeded translocation Cell 2014, 157(7): 1619-1631
A current overview of the team and further information about the research group can be found on the HIRI page.