Cellular Proteome Research

Pathogenic bacteria and viruses utilize and manipulate cellular processes of our immune system. The identification of protein functions in the human immune system that decisively control the progression of infections constitutes the central aim of the research group Cellular Proteomics at the HZI.

Leader

Proteome Analytics

This platform provides full service as well as proteome-scaled research in collaborative projects. We combine expertise in biochemistry, mass spectrometry (MS) and biostatistics matching the demands in translational research and systems level approaches. The platform focuses on the characterization of proteins that are the functional players in infections and our prime targets in drug research. The platform contributes to the discovery of novel drug-targets in pathogen-host interactions and cellular signaling pathways that coordinate the course of infections.

We support

  • De novo protein analytics (ETD/MSn)
  • Label- and label-free proteome analytics
  • Molecular phenotyping of tissues and cells
  • Donor- & patient-specific profilings
  • Interactome studies (IP-MS)
  • Post-translational modification analyses (phosphorylation, ubiquitination, glycosylation)
  • Drug target identifications

Equipment

The platform utilizes modern MS workflows including Orbitrap (Velos Pro & ETD-Fusion), SILAC-, iTRAQ- and TMT labeling technologies and various in house software packages and databases (e.g. ProteomDiscoverer, MaxQuant, OpenMS). Bioinformatics and Biostatistics in clinical and translational projects is further supported by the project of Prof. Frank Klawonn and our collaboration with Prof. O. Schilling and Dr. Lars Nilse (Institute of Molecular Medicine and Cell Research, Freiburg). Proteome analyses can be started with a minimum of 2x10E5 sorted cells.

You are a clinician or scientist in infection and drug research with demands in protein analytics?

Please Contact:

  • Dr. Manfred Nimtz 
  • Dr. Josef Wissing 
  • Prof. Dr. Lothar Jänsch

News

Bachelor & Master
Are you interested in a bachelor or master thesis? We are looking forward to your request!

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