Synthetic Biology of RNA

RNA is a ubiquitous molecule of life that plays intimate roles in how cells function and make decisions. These same properties can be harnessed to create a new generation of engineering tools to further interrogate the properties of biology and control how cells behave. The RNA synthetic biology group aims to better understand the roles RNA plays in biology and to exploit these roles to improve how we study, diagnose, and treat infectious diseases in humans.

Leader

selected publications

Marshall R, Maxwell CS, Collins SP, Jacobsen T, Luo ML, Begemann MB, Gray BN, January E, Singer A, He Y, Beisel CL, Noireaux V
Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System
Mol Cell 2018, 69(1): 146-157

Leenay RT, Maksimchuk KR, Slotkowski RA, Agrawal RN, Gomaa AA, Briner AE, Barrangou R, Beisel CL
Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems
Mol Cell 2016, 62(1): 137-147

Luo ML1, Mullis AS1, Leenay RT1, Beisel CL
Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression
Nucleic Acids Res 2015, 43(1): 674-681

Gomaa AA, Klumpe HE, Luo ML, Selle K, Barrangou R, Beisel CL
Programmable removal of bacterial strains by use of genome-targeting CRISPR-Cas systems
MBio 2014, 5(1): e00928-13

Beisel CL, Storz G
The base-pairing RNA spot 42 participates in a multioutput feedforward loop to help enact catabolite repression in Escherichia coli
Mol Cell 2011, 41(3): 286-297

Recent publications

Dugar G, Leenay RT, Eisenbart SK, Bischler T, Aul BU, Beisel CL, Sharma CM
CRISPR RNA-Dependent Binding and Cleavage of Endogenous RNAs by the Campylobacter jejuni Cas9
Mol Cell 2018, 69(5): 893-905

Maxwell CS, Jacobsen T, Marshall R, Noireaux V, Beisel CL
A detailed cell-free transcription-translation-based assay to decipher CRISPR protospacer-adjacent motifs
Methods 2018, pii: S1046-2023(17)30339-0

Marshall R, Maxwell CS, Collins SP, Jacobsen T, Luo ML, Begemann MB, Gray BN, January E, Singer A, He Y, Beisel CL, Noireaux V
Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System
Mol Cell 2018, 69(1): 146-157

Marshall R, Maxwell CS, Collins SP, Beisel CL, Noireaux V
Short DNA containing χ sites enhances DNA stability and gene expression in E. coli cell-free transcription-translation systems
Biotechnol Bioeng 2017, 114(9): 2137-2141

Waller MC, Bober JR, Nair NU, Beisel CL
Toward a genetic tool development pipeline for host-associated bacteria
Curr Opin Microbiol 2017, 38: 156-164

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