Nanoscale Infection Biology

Viruses are nanoscale entities. Despite their size and low complexity, they efficiently enter host cells leading to infection and reprogramming of cellular functions. The critical processes involve only a handful of viral and cellular proteins. Yet this contact is critical for the outcome of infection and the cellular immune response. We look at these processes to understand which cellular processes are stimulated by viruses and how the host cell interprets an infecting virus at the molecular level. At the scale of single viruses, these processes, their dynamics and structural conditions remain mostly unclear. We thus use advanced microscopy techniques, which allow us to visualize viral and cellular nanostructures during the infection process.

Leader

Selected Publications

Sieben, C.#, Sezgin, E., Eggeling, C. and Manley, S.#, 2020. Influenza A viruses use multivalent sialic acid clusters for cell binding and receptor activation. PLoS Pathogens, 16(7), p.e1008656.
https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1008656

Koehler, M., Delguste, M., Sieben, C., Gillet, L. and Alsteens, D., 2020. Initial Step of Virus Entry: Virion Binding to Cell-Surface Glycans.
https://www.annualreviews.org/doi/full/10.1146/annurev-virology-122019-070025

Sieben, C.*,#, Banterle, N., Douglass, K.M., Gönczy, P. and Manley, S.#, 2018. Multicolor single-particle reconstruction of protein complexes. Nature Methods, 15(10), pp.777-780.
https://www.nature.com/articles/s41592-018-0140-x?WT.feed_name=subjects_structure-determination

Schelker, M., Mair, C.M., Jolmes, F., Welke, R.W., Klipp, E., Herrmann, A., Flöttmann, M.# and Sieben, C.#, 2016. Viral RNA degradation and diffusion act as a bottleneck for the influenza A virus infection efficiency. PLoS Computational Biology, 12(10), p.e1005075.
https://journals.plos.org/ploscompbiol/article/comments?id=10.1371/journal.pcbi.1005075

Sieben, C.*, Kappel, C.*, Zhu, R., Wozniak, A., Rankl, C., Hinterdorfer, P., Grubmüller, H. and Herrmann, A., 2012. Influenza virus binds its host cell using multiple dynamic interactions. PNAS, 109(34), pp.13626-13631.
​​​​​​​https://www.pnas.org/content/109/34/13626

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