Molecular Cell Biology

The cytoskeleton is responsible for mechanical stabilisation of the cell, for its motility and intracellular transport processes as well as for maintenance and change of its overall shape. The research group Molecular Cell Biology focusses on one specific part of the so called cytoskeleton: the actin cytoskeleton. The dynamics and turnover of filaments of the actin cytoskeleton are particularly relevant for effective immune responses, and are also frequently targeted by pathogens. Understanding the molecular mechanisms mediating assembly and disassembly of this filament system is thus among the main goals of Klemens Rottner and his team.


Selected Publications

Rottner, K., Stradal, T.E.B., Chen, B. (2021) WAVE regulatory complex. Curr Biol, 31(10):R512-R517. DOI: 10.1016/j.cub.2021.01.086. 

Dimchev, V., Lahmann, I., Koestler, S.A., Kage, F., Dimchev, G., Steffen, A., Stradal, T.E.B., Vauti, F., Arnold, H.H., Rottner, K. (2021) Induced Arp2/3 complex depletion increases FMNL2/3 formin expression and filopodia formation. Front Cell Dev Biol, 9:634708. DOI: 10.3389/fcell.2021.634708. 

Dimchev, G., Amiri, B., Humphries, A.C., Schaks, M., Dimchev, V., Stradal, T.E., Faix, J., Krause, M., Way, M., Falcke, M., Rottner, K. (2020) Lamellipodin tunes cell migration by stabilizing protrusions and promoting adhesion formation. J Cell Sci, 133(7):jcs239020.

Schaks, M., Singh, S.P., Kage, F., Thomason, P., Klünemann, T., Steffen, A., Blankenfeldt, W., Stradal, T.E., Insall, R., Rottner, K. (2018) Distinct interaction sites of Rac GTPase with WAVE regulatory complex have non-redundant functions in vivo. Curr Biol, 28(22):3674-3684. doi: 10.1016/j.cub.2018.10.002.

Kage, F., Winterhoff, M., Dimchev, V., Mueller, J., Thalheim, T., Freise, A., Brühmann, S., Kollasser, J., Block, J., Dimchev, G., Geyer, M., Schnittler, H.-J., Brakebusch, C., Stradal, T.E., Carlier, M.-F., Sixt, M., Käs, J., Faix, J., Rottner, K. (2017) FMNL formins boost lamellipodial force generation. Nat Commun, 8:14832.


Bieling, P., Rottner, K. (2023) From WRC to Arp2/3 - Collective molecular mechanisms of branched actin network assembly. Curr Opin Cell Biol, in press.

Castellanos-Martínez, R., León-Vega, I., Guerrero-Fonseca, I., Vargas-Robles, H., Jiménez-Camacho, K., Hernández-Galicia, G., Ortíz-Navarrete, V., Rottner, K., Medina-Contreras, Ó., Schnoor, M. (2023) T cell functions and organ infiltration by leukemic T cells require cortactin. J Leukoc Biol, in press.

Fäßler, F., Javoor, M.G., Datler, J., Döring, H., Hofer, F.W., Dimchev, G., Hodirnau, V.V., Faix, J., Rottner, K., Schur, F.K.M. (2023) ArpC5 isoforms regulate Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning. Sci Adv. 9(3):eadd6495. DOI: 10.1126/sciadv.add6495.

Pokrant, T., Hein, J.I., Körber, S., Disanza, A., Pich, A., Scita, G., Rottner, K., Faix, J. (2023) Ena/VASP clustering at microspike tips involves Lamellipodin but not I-BAR proteins, and absolutely requires unconventional Myosin-X. Proc Natl Acad Sci USA, 120(2):e2217437120. DOI: 10.1073/pnas.2217437120.

Sharafutdinov, I., Knorr, J., Rottner, K., Backert, S., Tegtmeyer, N. (2022) Cortactin: a universal host cytoskeletal target of Gram-negative and Gram-positive bacterial pathogens. Mol Microbiol, 118(6):623-636. DOI: 10.1111/mmi.15002. 

Yang, S., Tang, Y., Liu, Y., Brown, A.J., Schaks, M., Ding, B., Kramer, D.A., Mietkowska, M., Ding, L., Alekhina, O., Billadeau, D.D., Chowdhury, S., Wang, J., Rottner, K., Chen, B. (2022) Arf GTPase activates the WAVE Regulatory Complex through a distinct binding site. Sci Adv, 8(50):eadd1412. DOI: 10.1126/sciadv.add1412.

Kirchenwitz, M., Stahnke, S., Grunau, K., Melcher, L., van Ham, M., Rottner, K., Steffen, A., Stradal, T.E.B. (2022) The autophagy inducer SMER28 attenuates microtubule dynamics mediating neuroprotection. Sci Rep, 12(1):17805. DOI: 10.1038/s41598-022-20563-3.

Ding, B., Yang, S., Schaks, M., Liu, Y., Brown, A.J., Rottner, K., Chowdhury, S., Chen, B. (2022) Structures reveal a key mechanism of WAVE regulatory complex activation by Rac1 GTPase. Nat Commun, 13(1):5444. DOI: 10.1038/s41467-022-33174-3.

Kirchenwitz, M., Stahnke, S., Prettin, S., Borowiak, M., Menke, L., Sieben, C., Birchmeier, C., Rottner, K., Stradal, T.E., Steffen, A. (2022) SMER28 Attenuates PI3K/mTOR Signaling by Direct Inhibition of PI3K p110 Delta. Cells, 11(10):1648. DOI: 10.3390/cells11101648.

Kage, F., Döring, H., Mietkowska, M., Schaks, M., Grüner, F., Stahnke, S., Steffen, A., Müsken, M., Stradal, T.E., Rottner, K. (2022) Lamellipodia-like actin networks in cells lacking WAVE regulatory complex. J Cell Sci, 135(15):jcs260364. DOI: 10.1242/jcs.260364.

Lartey, N.L., Vargas-Robles, H., Guerrero-Fonseca, I.M., García-Ponce, A., Salinas-Lara, C., Rottner, K., Schnoor, M. (2022) The Actin-Binding Protein Cortactin Promotes Sepsis Severity by Supporting Excessive Neutrophil Infiltration into the Lung. Biomedicines, 10(5):1019. DOI: 10.3390/biomedicines10051019.

Petersen, L., Stroh, S., Schoettelndreier, D., Grassl, G., Rottner, K., Brakebusch, C., Fahrer, J., Genth, H. (2022) The essential role of Rac1 glucosylation in Clostridioides difficile Toxin B-induced arrest of G1-S transition. Front Microbiol, 13:846215. DOI: 10.3389/fmicb.2022.846215.

Fung, T.S., Chakrabarti, R., Kollasser, J., Rottner, K., Stradal, T.E., Kage, F., Higgs, H.N. (2022) Parallel kinase pathways stimulate actin polymerization at depolarized mitochondria. Curr Biol, 32(7):1577-1592.e8. DOI: 0.1016/j.cub.2022.02.058.

Kummer, D., Steinbacher, T., Thölmann, S., Schwietzer, M., Hartmann, C., Horenkamp, S., Demuth, S., Peddibhotla, S., Brinkmann, F., Kemper, B., Schnekenburger, J., Brandt, M., Betz, T., Liashkovich, I., Kouzel, I., Shahin, V., Corvaia, N., Rottner, K., Tarbashevich, K., Raz, E., Greune, L., Schmidt, M.A., Gerke, V., Ebnet, K. (2022) A JAM-A - tetraspanin - αvβ5 integrin complex regulates contact inhibition of locomotion. J Cell Biol, 221(4):e202105147. DOI: 10.1083/jcb.202105147.

Faix, J., Rottner, K. (2022) Ena/VASP proteins in cell edge protrusion, migration and adhesion. J Cell Sci, 135(6):jcs259226. DOI: 10.1242/jcs.259226.

Rottner, K., Stradal, T.E. (2022) WASP stings into matrix to lead immune cell migration. J Cell Biol, in press.

Mehidi, A., Kage, F., Karatas, Z., Cercy, M., Schaks, M., Polesskaya, A., Sainlos, M., Gautreau, A., Rossier, O., Rottner, K., Giannone, G. (2021) Forces generated by lamellipodial actin filament elongation regulate the WAVE complex during cell migration. Nature Cell Biol, 23(11):1148-1162. doi: 10.1038/s41556-021-00786-8.

Tegtmeyer, N., Harrer, A., Rottner, K., Backert, S. (2021) Helicobacter pylori CagA Induces Cortactin Y-470 Phosphorylation-Dependent Gastric Epithelial Cell Scattering via Abl, Vav2 and Rac1 Activation. Cancers (Basel), 13(16):4241. doi: 10.3390/cancers13164241.

Funk, J., Merino, F., Schaks, M., Rottner, K., Raunser, S., Bieling, P. (2021) A barbed end interference mechanism reveals how capping protein promotes nucleation in branched actin networks. Nat Commun, 12(1):5329. doi: 10.1038/s41467-021-25682-5.

Cornelius, J., Rottner, K., Korte, M., Michaelsen-Preusse, K. (2021) Cortactin contributes to activity-dependent modulation of spine actin dynamics and spatial memory formation. Cells,  10(7):1835. doi: 10.3390/cells10071835.

Walch, P., Selkrig, J., Knodler, L.A., Rettel, M., Stein, F., Fernandez, K., Viéitez, C., Potel, C.M., Scholzen, K., Geyer, M., Rottner, K., Steele-Mortimer, O., Savitski, M.M., Holden, D.W., Typas, A. (2021) Global mapping of Salmonella enterica-host protein-protein interactions during infection. Cell Host Microbe, 29(8):1316-1332.e12, doi: 10.1016/j.chom.2021.06.004.

Knorr, J., Sharafutdinov, I., Fiedler, F., Esmaeili, D.S., Rohde, M., Rottner, K., Backert, S., Tegtmeyer, N. (2021) Cortactin is required for efficient FAK, Src and Abl tyrosine kinase activation and phosphorylation of Helicobacter pylori CagA. Int J Mol Sci, 22(11):6045. doi: 10.3390/ijms22116045.

Rottner, K., Stradal, T.E.B., Chen, B. (2021) WAVE regulatory complex. Curr Biol, 31(10):R512-R517. DOI: 10.1016/j.cub.2021.01.086.

Stahnke, S., Döring, H., Kusch, C., de Gorter, D.J.J., Dütting, S., Guledani, A., Pleines, I., Schnoor, M., Sixt, M., Geffers, R., Rohde, M., Müsken, M., Kage, F., Steffen, A., Faix, J., Nieswandt, B., Rottner, K., Stradal, T.E.B. (2021) Loss of Hem1 disrupts macrophage function and impacts on migration, phagocytosis and integrin-mediated adhesion. Curr Biol, 31(10):2051-2064.e8.

Leithner, A., Altenburger, L.M., Hauschild, R., Assen, F., Rottner, K., Stradal, T.E., Diz-Muñoz, A., Stein, J.V., Sixt, M. (2021) Dendritic cell actin controls contact duration and priming efficiency at the immunological synapse. J Cell Biol, in press. DOI: 10.1083/jcb.202006081.

Dimchev, V., Lahmann, I., Koestler, S.A., Kage, F., Dimchev, G., Steffen, A., Stradal, T.E.B., Vauti, F., Arnold, H.H., Rottner, K. (2021) Induced Arp2/3 complex depletion increases FMNL2/3 formin expression and filopodia formation. Front Cell Dev Biol, 9:634708. DOI: 10.3389/fcell.2021.634708. 

Papalazarou, V., Swaminathan, K., Jaber-Hijazi, F., Spence, H.J., Lahmann, I., Nixon, C., Salmeron-Sanchez, M., Arnold, H.H., Rottner, K., Machesky, L.M. (2020) The Arp2/3 complex is critical for colonisation of the mouse skin by melanoblasts. Development, 147(22):dev194555.  DOI: 10.1242/dev.194555.

Singh, S.P., Thomason, P.A., Lilla, S., Schaks, M., Tang, Q., Goode, B.L., Machesky, L.M., Rottner, K., Insall, R.H. (2020) Cell-substrate adhesion drives Scar/WAVE activation and phosphorylation by a Ste20-family kinase, which controls pseudopod lifetime. PLoS Biol, 18(8):e3000774. DOI: 10.1371/journal.pbio.3000774.

Wang, C., Lambert, C., Hauser, M., Deuschmann, A., Zeilinger, C., Rottner, K., Stradal, T.E.B., Stadler, M., Skellam, E.J., Cox, R.J. (2020) Diversely Functionalised Cytochalasins via Mutasynthesis and Semi-Synthesis. Chemistry, 26(60):13578-13583. DOI: 10.1002/chem.202002241.

Tang, Q., Schaks, M., Koundinya, N., Yang, C., Pollard, L.W., Svitkina, T.M., Rottner, K., Goode, B.L. (2020) WAVE1 and WAVE2 have distinct and overlapping roles in controlling actin assembly at the leading edge. Mol Biol Cell, 31(20):2168-2178. 

Salzer, E., Zoghi, S., Kiss, M.G., Kage, F., Rashkova, C., Stahnke, S., Haimel, M., Platzer, R., Caldera, M., Ardy, R.C., Hoeger, B., Block, J., Medgyesi, D., Sin, C., Shakarami, S., Kain, R., Ziaee, V., Hammerl, P., Bock, C., Menche, J., Dupré, L., Huppa, J.B., Sixt, M., Lomakin, A., Rottner, K., Binder, C.J., Stradal, T.E.B., Rezaei, N., Boztug, K. (2020) The cytoskeletal regulator Hem1 governs B-cell development and prevents autoimmunity. Sci Immunol, 5(49):eabc3979.

Schaks, M., Reinke, M., Witke, W., Rottner, K. (2020) Molecular Dissection of Neurodevelopmental Disorder-Causing Mutations in CYFIP2. Cells, 9(6): e1355.

Damiano-Guercio, J., Kurzawa, L., Mueller, J., Dimchev, G., Schaks, M., Nemethova, M., Pokrant, T., Brühmann, S., Linkner, J., Blanchoin, L., Sixt, M., Rottner, K., Faix, J. (2020) Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion. Elife, 9:e55351.

Dimchev, G., Amiri, B., Humphries, A.C., Schaks, M., Dimchev, V., Stradal, T.E., Faix, J., Krause, M., Way, M., Falcke, M., Rottner, K. (2020) Lamellipodin tunes cell migration by stabilizing protrusions and promoting adhesion formation. J Cell Sci, 133(7):jcs239020.

Samus, M., Li, Y.-T., Sorokin, L., Rottner, K., Vestweber, D. (2020) Actin-binding protein cortactin promotes pathogenesis of experimental autoimmune encephalomyelitis by supporting leukocyte infiltration into the central nervous system. J Neurosci, 40(7):1389-1404.

Rottner, K., Schaks, M. (2019) N-WASP Guides Cancer Cells toward LPA. Dev Cell, 51(4):415-417.

Schaks, M., Giannone, G., Rottner, K. (2019) Actin dynamics in cell migration. Essays Biochem, 63(5):483-495.

Taha, M., Aldirawi, M., März, S., Seebach, J., Odenthal-Schnittler, M., Bondareva, O., Bojovic, V., Schmandra, T., Wirth, B., Mietkowska, M., Rottner, K., Schnittler, H. (2019) EPLIN-α and -β Isoforms Modulate Endothelial Cell Dynamics Through a Spatio-Temporally Differentiated Interaction with Actin. Cell Rep, 29(4):1010-1026.e6.

Schaks, M. Döring, H., Kage, F., Steffen, A., Klünemann, T., Blankenfeldt, W., Stradal, T, Rottner, K. (2019) RhoG and Cdc42 can contribute to Rac-dependent lamellipodia formation through WAVE Regulatory Complex-binding. Small GTPases, published online Aug 26:1-11.,

Mehidi, M., Rossier, O., Schaks, M., Chazeau, A., Binamé, Remorino, A., Coppey, M., Karatas, Z., Sibarita, J.-B., Rottner, K., Moreau, V., Giannone, G. (2019) Transient activations of Rac1 at the lamellipodium tip trigger membrane protrusion. Curr Biol, 29(17):2852-2866.e5. doi: 10.1016/j.cub.2019.07.035, in press.

Kunschmann, T., Puder, S., Fischer, T., Steffen, A., Rottner, K., Mierke, C.T. (2019) The Small GTPase Rac1 Increases Cell Surfaces Stiffness and Enhances 3D Migration into Extracellular Matrices, Sci Rep, 9(1):7675.

Velázquez-Avila, M., Balandrán, J.C., Ramírez, D., Velázquez-Avila, M., Sandoval-Cabrera, A., Felipe-López, A., Nava, P., Alvarado-Moreno, J., Dozal, D., Prieto-Chávez, J., Schaks, M., Rottner, K., Dorantes-Acosta, E.M., Lopez-Martinez, B., Schnoor, M., Pelayo, R. (2019) High cortactin expression in B-cell acute lymphoblastic leukemia is associated with increased transendothelial migration and bone marrow relapse. Leukemia, 33(6):1337-1348. doi: 10.1038/s41375-018-0333-4.

Kugelmann, D., Rötzer, V., Walter, E., Egu, D.T., Fuchs, M.T., Vielmuth, F., Vargas-Robles, H., Schnoor, M., Hertl, M., Eming, R., Rottner, K., Schmidt, A., Spindler, V., Waschke, J. (2019) Role of Src and cortactin in pemphigus skin blistering. Front Immunol, 10:626. doi: 10.3389/fimmu.2019.00626.  

Litschko, C., Brühmann, S., Csiszár, Stephan, T., Dimchev, V., Damiano-Guercio, J., Junemann, A., Körber, S., Winterhoff, M., Nordholz, B., Ramalingam, N., Peckham, M., Rottner, K., Merkel, R., Faix, J. (2019) Functional integrity of the contractile actin cortex is safeguarded by multiple Diaphanous-related formins. Proc Natl Acad Sci USA, 116(9):3594-3603. doi: 10.1073/pnas.1821638116.

Rottner, K., Schaks, M. (2019) Assembling actin filaments for protrusion, Curr Opin Cell Biol, 56:53-63. doi: 10.1016/

Schaks, M., Singh, S.P., Kage, F., Thomason, P., Klünemann, T., Steffen, A., Blankenfeldt, W., Stradal, T.E., Insall, R., Rottner, K. (2018) Distinct interaction sites of Rac GTPase with WAVE regulatory complex have non-redundant functions in vivo. Curr Biol, 28(22):3674-3684. doi: 10.1016/j.cub.2018.10.002.

Dolati, S., Kage, F., Mueller, J., Müsken, M., Kirchner, M., Dittmar, G., Sixt, M., Rottner, K., Falche, M. (2018) On the relation between filament density, force generation and protrusion rate in mesenchymal cell motility. Mol Biol Cell, 29(22):2674-2686, doi: 10.1091/mbc.E18-02-0082.

Beer, L.-A., Tatge, H., Reich, N., Tenspolde, M., Olling, A., Goy, S., Rottner, K., Alekov, A., Gerhard, R. (2018) Early cell death induced by Clostridium difficile TcdB: Uptake and Rac1-glucosylation kinetics are decisive for cell fate. Cell Microbiol, e12865, doi: 10.1111/cmi.12865.

Ge, J., Burnier, L., Adamopoulou, M., Kwa, M., Schaks, M., Rottner, K., Brakebusch, C. (2018) RhoA, Rac1 and Cdc42 differentially regulate aSMA and collagen I expression in mesenchymal stem cells. J Biol Chem, 293(24):9358-69.

Dimchev, G., Rottner, K. (2018) Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators. J Vis Exp, (135). doi: 10.3791/57643.

Steffen, K., Kage, F., Rottner, K. (2018) Imaging the molecular machines that power cell migration. Methods Molecular Biology, Vol. 1749, Alexis Gautreau (Ed):Cell Migration, 257-277.

Schnoor, M., Stradal, T.E., Rottner, K. (2018) Cortactin: cell functions of a multi-faceted actin-binding protein. Trends Cell Biol, 28(2):79-98.

Rottner, K., Kage, F. (2017) Actin Networks: Adapting to Load through Geometry. Curr Biol, 27(23):R1274-77.

Rottner, K., Faix, J., Bogdan, S., Linder, S., Kerkhoff, E. (2017) Actin assembly mechanisms at a glance. J Cell Sci, 130(20):3427-35.

Böttcher, R.T., Veelders, M., Rombaut, P., Faix, J., Theodosiou, M., Stradal, T.E., Rottner, K., Zent, R., Herzog, F., Faessler, R. (2017) Kindlin-2 recruits paxillin and Arp2/3 to promote membrane protrusions during initial cell spreading. J Cell Biol, 216(11):2385-98.

Litschko, C., Linkner, J., Brühmann, S., Stradal, T.E., Reinl, T., Jänsch, L., Rottner, K., Faix, J. (2017) Differential functions of WAVE regulatory complex subunits in the regulation of actin-driven processes. Europ J Cell Biol, 96(8):715-27.

Kage, F., Steffen, A., Ellinger, A., Ranftler, C., Gehre, C., Brakebusch, C., Pavelka, M., Stradal, T.E., Rottner, K., Faix, J., (2017) FMNL2 and -3 regulate Golgi architecture and anterograde transport downstream of Cdc42. Sci Rep, 7:9791. Weblink:

Dimchev, G., Steffen, A., Kage, F., Dimchev, V., Pernier, J., Carlier, M.-F., Rottner, K. (2017) Efficiency of lamellipodia protrusion is determined by the extent of cytosolic actin assembly. Mol Biol Cell, 28(10):1311-1325.

Kage, F., Winterhoff, M., Dimchev, V., Mueller, J., Thalheim, T., Freise, A., Brühmann, S., Kollasser, J., Block, J., Dimchev, G., Geyer, M., Schnittler, H.-J., Brakebusch, C., Stradal, T.E., Carlier, M.-F., Sixt, M., Käs, J., Faix, J., Rottner, K. (2017) FMNL formins boost lamellipodial force generation. Nat Commun, 8:14832.

Citalán Madrid, A.F., Vargas Robles, H., García Ponce, A., Shibayama, M., Betanzos, A., Nava, P., Salinas-Lara, C., Rottner, K., Menningen, R., Schnoor, M. (2017) Cortactin deficiency causes increased RhoA/ROCK1-dependent actomyosin contractility, intestinal epithelial barrier dysfunction and disproportionately severe DSS-inducted colitis. Mucosal Immunol, 10(5):1237-1247.

Woodham, E.F., Paul, N.R., Tyrell, B., Spence, H.J., Swaminathan, K., Scribner, M.R., Giampazolias, E., Hedley, A., Clark, W., Kage, F., Marston, D.J., Hahn, K.M., Tait, S.W., Larue, L., Brakebusch, C.H., Insall, R.H., Machesky, L.M. (2017) Coordination by Cdc42 of Actin, Contractility, and Adhesion for Melanoblast Movement in Mouse Skin. Curr Biol, 27(5):624-37.

Steffen, A., Stradal, T.E., Rottner, K. (2017) Signalling Pathways Controlling Cellular Actin Organization. Handb Exp Pharmacol, 235:153-78.

Leithner, A., Eichner, A., Müller, J., Reversat, A., Brown, M., Schwarz, J., Merrin, J., de Gorter, D., Schur, F., Bayerl, J., de Vries, I., Wieser, S., Hauschild, R., Lai, F.P., Moser, M., Kerjaschki, D., Rottner, K., Small, J.V., Stradal, T.E., Sixt, M. (2016) Diversified actin protrusions promote environmental exploration but are dispensable for locomotion of leukocytes. Nat Cell Biol, 18(11):1253-9.

Andritschke, D., Dilling, S., Emmenlauer, M., Weltz, T., Schmich, F., Misselwitz, B., Rämö, P., Rottner, K., Kerkhoff, E., Wada, T., Penninger, J.M., Beerenwinkel, N., Horvath, P., Dehio, C., Hardt, W.-D. (2016) A genome-wide siRNA screen implicates Spire1/2 in SipA-driven Salmonella Typhimurium host cell invasion. PLoS ONE, 11(9):e0161965.

García Ponce, A., Citalán Madrid, A.F., Vargas Robles, H., Cánez Paredes, S., Nava P., Betanzos, A., Zabrock, A., Rottner, K., Vestweber, D., Schnoor, M. (2016) Loss of cortactin causes endothelial barrier dysfunction via dicturbed adrenomedullin secretion and actomyosin contractility. Sci Rep, 6:29003.

Rottner, K. (2016) Formin’ filaments at a faster CLIP. Science, 352(6288):894-5.

Wollscheid, H.P., Biancospino, M., He, F., Magistrati, E., Molteni, E., Lupia, M., Soffientini, P., Rottner, K., Cavallaro, U., Pozzoli, U., Mapelli, M., Walters K.J., Polo, S. (2016) Diverse functions of Myosin VI educidated by an isoform-specific a-Helix Domain. Nat Struct Mol Biol, 23(4):300-8

Rottner, K., Stradal, T.E.B. (2016) How distinct Apr2/3 complex variants regulate actin filament assembly. Nat Cell Biol, 18(1):1-3.

Kühn, S., Erdmann, C., Kage, F., Block, J., Schwenkmezger, L., Steffen, A., Rottner, K., Geyer, M. (2015) The structure of FMNL2-Cdc42 yields insights into the mechanism of lamellipodia and filopodia formation. Nat Commun, 6:7088.

Chazeau, A., Mehidi, A., Nair, D., Gautier, J., Leduc, C., Chamma, I., Kage, F., Kechkar, A., Thoumine, O., Rottner, K., Choquet, D., Gautreau, A., Sibarita, J.-B., Giannone, G. (2014) Nanoscale segregation of actin nucleation and elongation factors determines dendritic spine protrusion. EMBO J, 33(23):2745-64.

May, M., Schelle, I., Brakebusch, C., Rottner, K., Genth H. (2014) Rac1-dependent recruitment of PAK2 to G2 phase centrosomes and their roles in the regulation of mitotic entry. Cell Cycle, 13(14):2210-20.

Steffen, A., Koestler, S.A., Rottner, K. (2014) Requirements for and Consequences of Rac-dependent Protrusion. Eur J Cell Biol, 93(5-6):184-93.

Biosse-Duplan, M., Zalli, D., Stephens, S., Zenger, S, Neff, L., Holleboom, J.M., Lai, F.P.L., Horne, W., Rottner, K., Baron, R. (2014) Microtubule Dynamic Instability Controls Podosome Patterning in Osteoclasts through EB1, Cortactin and Src. Mol Cell Biol, 34(1):16-29.

Dang, I., Gorelik, R., Sousa, C., Linkner, J., Guérin, C., Nemethova, M., Chipysheva, T., Ermilova, V., Vacher, S., Herrada, I., Campanacci, V., Derivery, E., Steffen, A., Lakisic, G., Rottner, K., Cherfils, J., Zinn-Jutsin, S., Bièche, I., Alexandrova, A., Small, J.V., Blanchoin, L., Faix, J., Gautreau, A. (2013) Inhibitory signalling to the Arp2/3 complex steers cell migration, Nature, 503(7475):281-4.

Koestler, S.A., Steffen, A., Nemethova, M., Winterhoff, M., Luo, N., Holleboom, J.M., Krupp, J., Jacob, S., Vinzenz, M., Schur, F., Schlüter, K., Gunning, P.W., Winkler, C., Schmeiser, C., Faix, J., Stradal, T.E.B., Small, J.V., Rottner, K. (2013) Arp2/3 complex is essential for actin network treadmilling as well as for targeting of capping protein and cofilin. Mol Biol Cell, 24(18):2861-75.

Steffen, A., Ladwein, M., Dimchev, G.A., Hein, A., Schwenkmezger, L., Arens, S., Ladwein, K.I., Holleboom, J.M., Schur, F., Small, J.V., Schwarz, J., Gerhard, R., Faix, J., Stradal, T.E.B., Brakebusch, C., Rottner, K. (2013) Rac function is critical for cell migration but not required for spreading and focal adhesion formation. J Cell Sci, 126(20):4572-88.

Junemann, A., Winterhoff, M., Nordholz, B., Rottner, K., Eichinger, L., Gräf, R., Faix, J. (2013) ForC lacks canonical formin activity but bundles actin filaments and is required and multicellular development of Dictyostelium cells. Eur J Cell Biol, 92(6-7):201-12.

Wolters, M., Boyle, E.C., Lardong, K., Truelzsch, K., Steffen, A., Rottner, K., Ruckdeschel, K., Aepfelbacher, M. (2013) Cytotoxic necrotizing factor-Y boosts Yersinia effector translocation by activating Rac. J Biol Chem, 288(32):23543-53.

Huelsenbeck, S.C., Roggenkamp, D., May, M., Huelsenbeck, J., Brakebusch, C., Rottner, K., Ladwein, M., Just, I., Fritz, G., Schmidt, G., Genth, H. (2013) Expression and cytoprotective activity of the small GTPase RhoB induced by the Escherichia coli cytotoxic necrotizing factor 1. Int J Biochem Cell Biol, 45(8):1767-75.

Vestweber, D., Zeuschner, D., Rottner, K., Schnoor, M. (2013) Cortactin regulates the activity of small GTPases and ICAM-1 clustering in endothelium: implications for the formation of docking structures. Tissue Barriers, 1(1): e23862.

Thoss, F., Dietrich, F., Punkt, K., Illenberger, S., Rottner, K., Himmel, M., Ziegler, W.H. (2013) Metavinculin: New Insights into Functional Properties of a Muscle Adhesion Protein. Biochem Biophys Res Commun, 430(1):7-13.

Schwarz, J., Proff, J., Hävemeier, A., Ladwein, M., Rottner, K., Barlag, B., Pich, A., Tatge, H., Just, I., Gerhard, R. (2012) Serine-71 phosphorylation of Rac1 modulates downstream signaling. PLoS ONE, 7(9):e44358.

Vinzenz, M., Nemethova, M., Schur, F., Mueller, J., Narita, A., Urban, E., Winkler, C., Schmeiser, C., Köstler, S.A., Rottner, K., Resch, G.P., Maeda, Y., Small, J.V. (2012) Actin branching in the initiation and maintenance of lamellipodia. J Cell Sci, 125(11): 2775-85.

Block, J., Breitsprecher, D., Kühn, S., Winterhoff, M., Kage, F., Geffers, R., Duwe, P., Rohn, J.L., Baum, B., Brakebusch, C., Geyer, M., Stradal, T.E.B., Faix, J., Rottner, K. (2012) FMNL2 drives actin-based protrusion and migration downstream of Cdc42. Curr Biol, 22(11):1005-12.

Hänisch, J., Stradal, T.E.B., Rottner, K. (2012) A novel contractility pathway operating in Salmonella invasion. Virulence, 3(1):81-6.

Rottner, K., Stradal, T.E.B. (2011) Actin Dynamics and Turnover in Cell Motility. Curr Opin Cell Biol, 23(5):569-78.

Schnoor, M., Lai, F.P.L., Zarbock, A., Kläver, R., Polaschegg, C., Schulte, D., Weich, H.A., Oelkers, J.M., Rottner, K., Vestweber, D. (2011) Cortactin deficiency is associated with reduced neutrophil recruitment but increased vascular permeability in vivo. J Exp Med, 208(8):1721-35.

Breitsprecher, D., Koestler, S.A., Chizhov, I., Nemethova, M., Mueller, J., Goode, B.L., Small, J.V., Rottner, K., Faix, J. (2011) Cofilin cooperates with fascin to disassemble filopodial actin filaments. J Cell Sci, 124(19):3305-18.

Oelkers, J.M., Vinzenz, M., Nemethova, M., Jacob, S., Lai, F.P.L., Block, J., Szczodrak, M., Kerkhoff, E., Backert, S., Schlüter, K., Stradal, T.E.B., Small, J.V., Koestler, S.A., Rottner, K. (2011) Microtubules as platforms for assaying actin polymerization in vivo. PLoS ONE, 6(5):e19931.

Dubielecka, P.M., Ladwein, K.I., Xiong, X., Migeotte, I., Chorzalska, A., Anderson, K.V., Sawicki, J.A., Rottner, K., Stradal, T.E.B., Kotula, L. (2011) Essential role for Abi1 in embryonic survival and WAVE2 complex integrity. Proc Natl Acad Sci USA, 108(17):7022-7.

Hänisch, J., Kölm, R., Wozniczka, M., Bumann, D., Rottner, K., Stradal, T.E.B. (2011) Activation of a RhoA/Myosin II-dependent but Arp2/3-complex-independent pathway facilitates Salmonella invasion. Cell Host & Microbe, 9(4):273-85.

Jackson, B., Peyrollier, K., Pedersen, E., Basse, A., Karlsson, R., Wang, Z., Lefever, T., Ochsenbein, A., Schmidt, G., Aktories, A., Stanley, A:, Quondamatteo, F., Ladwein, M., Rottner, K., van Hengel, J., Brakebusch, C. (2011) RhoA is dispensable for skin development, but crucial for contraction and directed migration of keratinocytes. Mol Biol Cell, 22(5):593-605.

Rottner K., Hänisch J., Campellone K.G. (2010) WASH, WHAMM and JMY: regulation of Arp2/3 complex and beyond. Trends Cell Biol, 20(11):650-61.

Marg, S., Winkler, U., Sestu, M., Himmel, M., Schönherr, M., Bär, J., Mann, A., Moser, M., Mierke, C., Rottner, K., Blessing, M., Hirrlinger, J., Ziegler, W.H. (2010) The Vinculin-In20/21 Mouse: Characteristics of a Constitutive, Actin-Binding Deficient Splice Variant of Vinculin. PLoS One, 5(7):e11530.

Hertzog, M., Milanesi, F., Hazelwood, L., Disanza, A., Liu, H.J., Perlade, E., Malabarba, M.G., Pasqualato, S., Maiolica, A., Confalonieri, S., Le Clainche, C., Offenhauser, N., Block, J., Rottner, K., Di Fiore, P.P., Carlier, M.F., Volkmann, N., Hanein, D., Scita, G. (2010) Molecular basis for the dual function of Eps8 on actin dynamics: bundling and capping. PLOS Biol, 8(6):e1000387.

Klink, B.U., Barden, S., Heidler, T.V., Borchers, C., Ladwein, M., Stradal, T.E.B., Rottner, K., Heinz, D.W. (2010) Structure of Shigella IpgB2 in complex with human RhoA: Implications for the mechanism of bacterial guanine nucleotide exchange factor mimicry. J Biol Chem, 285(22):17197-208.

Hänisch, J., Ehinger, J., Ladwein, M., Rohde, M., Derivery, E., Bosse, T., Steffen, A., Bumann, D., Misselwitz, B., Hardt, W.-D., Gautreau, A., Stradal, T.E.B., Rottner, K. (2010) Molecular dissection of Salmonella-induced membrane ruffling versus invasion. Cell Microbiol, 12(1):84-98.

Rottner, K., Stradal, T.E.B. (2009) Poxviruses Taking a Ride on Actin: New Users of Known Hardware. Cell Host & Microbe, 6(6):697-9.

Lai, F.P.L., Szczodrak, M., Oelkers, J.M., Ladwein, M., Acconcia, F., Benesch, S., Auinger, S., Faix, J., Small, J.V., Polo, S., Stradal, T.E.B., Rottner, K. (2009) Cortactin promotes migration and platelet-derived growth factor-induced actin reorganization by signaling to Rho-GTPases. Mol Biol Cell, 20(14):3209-23.

Faix, J., Breitsprecher, D., Stradal, T.E.B., Rottner, K. (2009) Filopodia: complex models for simple rods. Int J Biochem Cell Biol, 41(8-9):1656-64.

Himmel, M., Ritter, A., Rothemund, S., Pauling, B.V., Rottner, K., Gingras, A.R., Ziegler, W.H. (2009) Control of high affinity interactions in the talin C-terminus – how talin domains coordinate protein dynamics in cell adhesions. J Biol Chem, 284(20):13832-42

Weiss, S.M., Ladwein, M., Schmidt, D., Ehinger, J., Lommel, S., Städing, K., Beutling, U., Disanza, A., Frank, R., Jänsch, L., Scita, G., Gunzer, F., Rottner, K., Stradal, T.E.B. (2009) IRSp53 links the enterohemorrhagic E. coli effectors Tir and EspFU for actin pedestal formation. Cell Host & Microbe, 5(3):244-58.

Koestler, S.A., Rottner, K., Lai, F., Block, J., Vinzenz, M., Small, J.V. (2009) F- and G-actin concentrations in lamellipodia of moving cells. PLoS One, 4(3):e4810.

Halabi-Cabezon, I., Huelsenbeck, J., May, M., Ladwein, M., Rottner, K., Just, I., Genth, H. (2008) Prevention of the cytopathic effect induced by Clostridium difficile Toxin B by active Rac1 FEBS Lett, 582(27):3751-6.

Steffen, A., Le Dez, G., Poincloux, R. Recchi, C., Nassoy, P., Rottner, K., Galli, T., Chavrier, P. (2008) MT1-MMP-dependent invasion is regulated by TI-VAMP/VAMP7. Curr Biol, 18(12):926-31.

Block, J., Stradal, T.E.B., Haenisch, J., Geffers, R., Koestler, S.A., Urban, E., Small, J.V., Rottner, K., Faix, J. (2008) Filopodia formation induced by active Dia2/Drf3. J Microsc, 231(3):506-17.

Ladwein, M., Rottner, K. (2008) On the Rho’d: The regulation of membrane protrusions by Rho-GTPases. FEBS Lett, 582(14):2066-74.

Lai, F.P.L., Szczodrak, M., Block, J., Faix, J., Breitsprecher, D., Mannherz, H.G., Stradal, T.E.B., Dunn, G.A., Small, J.V., Rottner, K. (2008) Arp2/3-complex interactions and actin network turnover in lamellipodia. EMBO J, 27(7):982-92.

Koestler, S.A., Auinger, S., Vinzenz, M., Rottner, K., Small, J.V. (2008) Differentially oriented populations of actin filaments generated in lamellipodia collaborate in pushing and pausing at the cell front. Nat Cell Biol, 10(3):306-13.

Bosse, T., Ehinger, J., Czuchra, A., Benesch, S., Steffen, A., Wu, X., Schloen, K., Niemann, H.H., Scita, G., Stradal, T.E.B., Brakebusch, C., Rottner, K. (2007) Cdc42 and phosphoinositide 3-kinase drive Rac-mediated actin polymerization downstream of c-Met in distinct and common pathways. Mol Cell Biol, 27(19):6615-28.

Müller-Taubenberger, A., Vos, M.J., Böttger, A., Lasi, M., Lai, F.P.L., Fischer, M., Rottner, K. (2006) Monomeric red fluorescent protein variants used for imaging studies in different species. Europ J Cell Biol, 85(9-10):1119-29.

Caspani, E.M., Echevarria, D., Rottner, K., Small, J.V. (2006) Live imaging of glioblastoma cells in brain tissue shows requirement of actin bundles for migration. Neuron Glia Biol, 2(2):105-14.

Steffen, A., Faix, J., Resch, G.P., Linkner, J., Wehland, J., Small, J.V., Rottner, K., Stradal, T.E.B. (2006) Filopodia formation in the absence of functional WAVE- and Arp2/3-complex. Mol Biol Cell, 17(6):2581-91.

Faix, J., Rottner, K. (2006) The making of filopodia. Curr Opin Cell Biol, 18(1):18-25.

Stamm, L., Pak, M.A., Morisaki, J.H., Snapper, S.B., Rottner, K., Lommel, S., Brown, E.J. (2005) Role of the WASP family proteins for Mycobacterium marinum actin tail formation. Proc Natl Acad Sci USA, 102(41):14837-42.

Innocenti, M., Gerboth, S., Rottner, K., Lai, F.P.L., Hertzog, M., Stradal, T.E.B., Frittoli, E., Didry, D., Polo, S., Disanza, A., Benesch, S., Di Fiore, P.P., Carlier, M.F., Scita, G. (2005) Abi1 regulates the activity of N-WASP and WAVE in distinct actin-based processes. Nat Cell Biol, 7(10):969-76.

Czuchra, A., Wu, X., Meyer, H., Van Hengel, J., Schroeder, T., Geffers, R., Rottner, K., Brakebusch, C. (2005) Cdc42 is not essential for filopodium formation, directed migration, cell polarization and mitosis in fibroblastoid cells. Mol Biol Cell, 16(10):4473-84.

Rottner, K., Stradal, T.E.B., Wehland, J. (2005) Bacteria – host-cell interactions at the plasma membrane: stories on actin cytoskeleton subversion. Dev Cell, 9:3-17.

Benesch, S., Polo, S., Lai, F.P.L., Anderson, K.I., Stradal, T.E.B., Wehland, J., Rottner, K. (2005) N-WASP deficiency impairs EGF internalization and actin assembly at clathrin coated pits. J Cell Sci, 118(14):3103-15.

Disanza, A., Steffen, A., Hertzog, M., Frittoli, E., Rottner, K., Scita, G. (2005) Actin polymerization machinery: the finish line of signaling networks, the starting point of cellular movement. Cell Mol Life Sci, 62(9):955-70.

Rottner, K., Lommel, S., Wehland, J., Stradal, T.E.B. (2004) Pathogen-induced actin filament rearrangement in infectious diseases. J Pathol,204(4):396-406.

Pacholsky, D., Vakeel, P., Himmel, M., Löwe, T., Stradal, T.E.B., Rottner, K., Fürst, D.O., van der Ven, P.F. (2004) Xin repeats define a novel actin-binding motif. J Cell Sci, 117:5257-68.

Stradal, T.E.B., Rottner, K., Disanza, A., Confalonieri, S., Innocenti, M., Scita, G. (2004) Regulation of actin dynamics by WASP and WAVE family proteins. Trends Cell Biol, 14(6):303-11.

Lommel, S., Benesch, S., Rohde, M., Wehland, J., Rottner, K. (2004) Enterohaemorrhagic and enteropathogenic Escherichia coli use different mechanisms for actin pedestal formation that converge on N-WASP. Cell Microbiol, 6(3):243-54.

Steffen, A., Rottner, K., Ehinger, J., Innocenti, M., Scita, G., Wehland J., Stradal, T.E.B. (2004) Sra-1 and Nap1 link Rac to actin assembly driving lamellipodia formation. EMBO J, 23(4):749-59.

Breitbach, K., Rottner, K., Klocke, S., Rohde, M., Jenzora, A., Wehland, J., Steinmetz, I. (2003) Actin-based motility of Burkholderia pseudomallei involves the Arp2/3 complex, but not N-WASP and Ena/VASP proteins. Cell Microbiol, 5(6):385-93.

Benesch, S., Lommel, S., Steffen, A., Stradal, T.E.B., Scaplehorn, N., Way, M., Wehland, J., Rottner, K. (2002) Phosphatidylinositol 4,5-biphosphate (PIP2)-induced vesicle movement depends on N-WASP and involves Nck, WIP, and Grb2. J Biol Chem, 277:37771-6.

Small, J.V., Stradal, T., Vignal, E., Rottner, K. (2002) The lamellipodium: where motility begins. Trends Cell Biol, 12(3):112-20.

Rottner, K., Krause, M., Gimona, M., Small, J.V., Wehland, J. (2001) Zyxin is not colocalized with vasodilator-stimulated phosphoprotein (VASP) at lamellipodial tips and exhibits different dynamics to vinculin, paxillin, and VASP in focal adhesions. Mol Biol Cell, 12(10):3103-13.

Lommel, S., Benesch, S., Rottner, K., Franz, T., Wehland, J., Kühn, R. (2001) Actin pedestal formation by enteropathogenic Escherichia coli and intracellular motility of Shigella flexneri are abolished in N-WASP-defective cells. EMBO Rep, 2(9):850-7.

Stradal, T.E.B., Courtney, K.D., Rottner, K., Hahne, P., Small, J.V., Pendergast, A.M. (2001) The Abl interactor proteins localize to sites of actin polymerization at the tips of lamellipodia and filopodia. Curr Biol, 11(11):891-5.

Kohler, A., Jostarndt-Fögen, K., Rottner, K., Alliegro, M.C., Draeger, A. (1999) Intima-like smooth muscle.cells: developmental link between endothelium and media? Anat Embryol (Berl), 200(3):313-23.

Small, J.V., Rottner, K., Hahne, P., Anderson, K.I. (1999) Visualising the actin cytoskeleton. Microsc Res Tech, 47(1):3-17.

Rottner, K., Behrendt, B., Small, J.V., Wehland, J. (1999) VASP dynamics during lamellipodia protrusion. Nat Cell Biol, 1(5):321-2.

Small, J.V., Kaverina, I., Krylyshkina, O., Rottner, K. (1999) Cytoskeleton cross-talk during cell motility. FEBS Lett, 452(1-2):96-9.

Rottner, K., Hall, A., Small, J.V. (1999) Interplay between Rac and Rho in the control of substrate contact dynamics. Curr Biol, 9(12):640-8.

Small, J.V., Rottner, K., Kaverina, I. (1999) Functional design in the actin cytoskeleton. Curr Opin Cell Biol, 11(1):54-60.

Small, J.V., Rottner, K., Kaverina, I., Anderson, K.I. (1998) Assembling an actin cytoskeleton for cell attachment and movement. Biochim Biophys Acta, 1404(3):271-81.

Kaverina, I., Rottner, K., Small, J.V. (1998) Targeting, capture, and stabilization of microtubules at early focal adhesions. J Cell Biol, 142(1):181-90.

Small, J.V., Anderson, K., Rottner, K. (1996) Actin and the coordination of protrusion, attachment and retraction in cell crawling. Biosci Rep, 16(5):351-68.

Bernroider, G., Holztrattner, M., Rottner, K. (1996) Sex steroid-opioid interactions associated with the temporal component of avian calling patterns. Horm Behav, 30(4):583-9.

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