Microbial Proteomics

Fuchs, S., M. Kucklick, E. Lehmann, A. Beckmann, M. Wilkens, B. Kolte, A. Mustafayeva, T. Ludwig, M. Diwo, J. Wissing, L. Jänsch, C.H. Ahrens, Z. Ignatova, and S. Engelmann. 2021. Towards the characterization of the hidden world of small proteins in Staphylococcus aureus, a proteogenomics approach. PloS Genetics. 17(6):e1009585. doi: 10.1371/journal.pgen.1009585.

Bartholomäus, A., B. Kolte, A. Mustafayeva, I. Goebel, S. Fuchs, D. Benndorf, S. Engelmann, Z. Ignatova. 2021. smORFer: a modular algorithm to detect small ORFs in prokaryotes. Nuc. Acid Res. 49:e89. doi: 10.1093/nar/gkab477.

Pagels M., Fuchs S., Pané-Farré J., Kohler C., Menschner L., Hecker M., McNamarra P.J., Bauer M.C., von Wachenfeldt C., Liebeke M., Lalk M., Sander G., von Eiff C., Proctor R.A., Engelmann S. (2010) Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in S. aureus. Mol Microbiol 76:1142-1161. 

Selle M., Hertlein  T., Oesterreich B., Klemm T., Kloppot P., Müller E., Ehricht R., Stentzel S., Bröker B.M., Engelmann S., Ohlsen K. (2016) Global antibody response to S. aureus live-cell vaccination. Sci Rep 6:24754.

Boedeker C., Schüler M., Reintjes G., Jeske O., van Teeseling M., Jogler M., Rast P., Borchert D., Devos D.P., Kucklick M., Schaffer M., Kolter R., van Niftrik L., Engelmann S., Amann R., Rhode M., Engelhardt H., Jogler C. (2017) Determining the bacterial cell biology of Planctomycetes. Nat Commun 8:14853.

Froschauer, K., S. L. Svensson, R. Gelhausen, E. Fiore, P. Kible, A. Klaude, M. Kucklick, S. Fuchs, F. Eggenhofer, S. Engelmann, R. Backofen, C. M. Sharma. 2022. Complementary Ribo-Seq approaches map the translatome and provide a small protein census in the foodborne pathogen Campylobacter jejuni. bioRxiv. doi: 10.1101/2022.11.09.515450 

Becker, D., R. Weikard, A. Heimes, F. Hadlich, H.M. Hammon, M.M. Meyerholz, W. Petzl, H. Zerbe, H.J. Schuberth, M. Hoedemaker, M. Schmicke, S.Engelmann, C. Kühn. 2022. Allele-biased expression of the bovine APOB gene associated with the cholesterol deficiency defect suggests cis-regulatory enhancer effects of the LTR retrotransposon insertion. Sci. Rep. 12:13469. doi: 10.1038/s41598-022-17798-5.

Mayer, K., M. Kucklick, H. Marbach, M. Ehling-Schulz, S. Engelmann, T. Grunert. 2021. Within-host adaptation of Staphylococcus aureus in a bovine mastitis infection is associated with increased cytotoxicity. Int. J. Mol. Sci. 22:8840. doi: 10.3390/ijms22168840.

Bartholomäus, A., B. Kolte, A. Mustafayeva, I. Goebel, S. Fuchs, D. Benndorf, S. Engelmann, Z. Ignatova.2021.smORFer: a modular algorithm to detect small ORFs in prokaryotes. Nuc. Acid Res. 49:e89. doi: 10.1093/nar/gkab477.

Fuchs, S., M. Kucklick, E. Lehmann, A. Beckmann, M. Wilkens, B. Kolte, A. Mustafayeva, T. Ludwig, M. Diwo, J. Wissing, L. Jänsch, C.H. Ahrens, Z. Ignatova, and S. Engelmann. 2021. Towards the characterization of the hidden world of small proteins in Staphylococcus aureus, a proteogenomics approach. PloS Genetics. 17(6):e1009585. doi: 10.1371/journal.pgen.1009585.

Beier, N., M. Kucklick, S. Fuchs, A. Mustafayeva, M. Behringer, E. Härtig, D. Jahn, and S. Engelmann. 2021. Adaptation of Dinoroseobacter shibae to oxidative stress and the specific role of RirA. PloS One. 16:e0248865. doi: 10.1371/journal.pone.0248865.

Wang, H., N. Beier, C. Boedeker, H. Sztajer, P. Henke, M. Neumann-Schaal, J. Mansky, M. Rhode, J. Overmann, J. Petersen, F. Klawonn, M. Kucklick, S. Engelmann, J. Tomasch, an I. Wagner-Döbler. 2021. Dinoroseobacter shibae outer membrane vesicles are enriched for the chromosome dimer resolution site dif.mSystems 6:e00693-20. doi: 10.1128/mSystems.00693-20.

Rohmeier, L., W. Petzl, M. Koy, T. Eickhoff, A. Hülsebusch, S. Jander, L. Macias, A. Heimes, S. Engelmann, M. Hoedemaker, H.M. Seyfert, C. Kühn, H.J. Schuberth, H. Zerbe, and M.M. Meyerholz. 2020. In vivo model to study the impact of genetic variation on clinical outcome of mastitis in dairy heifers. BMC Vet. Res. 16: 33. doi: 10.1186/s12917-020-2251-8.

Heimes, A., J. Brodhagen, R. Weikhard, D. Becker, M. Meyerholz, W. Petzl, H. Zerbe, H.-J. Schuberth, M. Hoedemaker, M. Schmicke, S. Engelmann, and C. Kühn. 2020. Cows selected for divergent mastitis susceptibility display a differential liver transcriptome profile after experimental Staphylococcus aureus mammary gland inoculation, J. Dairy Sci., 10: 6364 – 6373. doi.org/10.3168/jds.2019-17612.

Heimes A., J. Brodhagen., R. Weikard., H.-M. Seyfert, D. Becker, M.M. Meyerholz, W. Petzl, H. Zerbe, M. Hoedemaker, L. Rohmeier, H.J. Schuberth, M. Schmicke, S. Engelmann, C. Kühn. 2020. Hepatic transcriptome analysis identifies divergent pathogen-specific targeting-strategies to modulate the innate immune system in response to intramammary infection. Front. Immunol. 11: 715. doi: 10.3389/fimmu.2020.00715.

Seffer, M.T., G. Eden., S. Engelmann, J.T. Kielstein. 2020. Elimination of Staphylococcus aureus from the bloodstream using a novel biomimetic sorbent haemoperfusion device, BMJ Case Rep., 13: e235262. doi: 10.1136/bcr-2020-235262.

Heimes, A., J. Brodhagen, R. Weikhard, H.M. Hammon, M.M. Meyerholz, W. Petzl, H. Zerbe, S. Engelmann, M. Schmicke, M. Hoedemaker, H.J. Schuberth, and C. Kühn. 2019. Characterization of functional traits with focus on udder health in heifers with divergent paternally inherited haplotypes on BTA18. BMC Vet. Res. 15:241. doi: 10.1186/s12917-019-1988-4.

Meyerholz, M.M., L. Rohmeier, T. Eickhoff, A. Hülsebusch, S. Jander, M. Linden, L. Macias, M. Koy, A. Heimes|, L. Gorríz-Martín, S. Engelmann, M. Schmicke, M. Hoedemaker, W. Petzl, H. Zerbe, H.-J. Schuberth, and C. Kühn. 2019. Genetic selection for bovine chromosome 18 haplotypes associated with divergent somatic cell score affects post-partum reproductive and metabolic performance. J. Dairy Sci. 102: 9983-9994. doi: 10.3168/jds.2018-16171.

Kohler, C., R.A. Proctor, A. S. Bayer, M.R. Yeaman, M. Lalk, S. Engelmann, and N.N. Mishra. 2019. Proteomic and membrane lipid correlated of reduced host defense peptide. Antibiotics. 8:E169. doi: 10.3390/antibiotics8040169.

Brodhagen, J., R. Weikard, U. Thom, A. Heimes, J. Günther, F. Hadlich, H. Zerbe, W. Petzl, M. Meyerholz, M. Hoedemaker, H.-J. Schuberth, S. Engelmann, and C. Kühn. 2019. Development and evaluation of a milk protein transcript depletion method for differential transcriptome analysis in mammary gland tissue. BMC Genomics. 20:400.

Seiß, E. A., A. Krone, P. Formaglio, O. Goldmann, S. Engelmann, B. Schraven, E. Medina, and A. J. Müller. 2019. Longitudal proliferation mapping in vivo reveals the impact of NADPH-oxidase-mediated dampening of Staphylococus aureus growth rates within neutrophils. Sci. Rep. 9:5703.

Thunberg, U., S, Hugosson, H. Fredlund, Y. Cai, R. Ehricht, S. Monnecke, E. Müller, S. Engelmann, and B. Söderquist. 2019. Anti-Staphylococcal humoral immune response in patients with chronic rhinosinusitis. Rhinology-online. 2:50-58. doi.org/10.4193/RHINOL/19.002

Lerch, M. F., S. M. K. Schoenfelder, G. Marincola, F.D.R. Wencker, M. Eckart, K. U. Förster, C. M. Sharma, K. M. Thormann, M. Kucklick, S. Engelmann, and W. Ziebuhr. 2019. A non-coding RNA from the intracellular adhesin (ica) locus of Staphylococcusepidermidis controls polysaccharide intracellular adhesin (PIA)-mediated biofilm formation. Mol. Microbiol. 111:1571-1591. doi: 10.1111/mmi.14238.

Graf, A., R.J. Lewis, S. Fuchs, M. Pagels, S. Engelmann, K. Riedel, and J. Pané-Farré. 2018. The hidden lipoproteome ofStaphylococcus aureus. Int. J. Med. Microbiol.  306:569-581

Pastrana, F.R., J. Neef, D.G.A.M. Koedijk, J. de Groot, D de Graaf, J. Duipmans, M.F. Jonkman, S. Engelmann, J.M. van Dijl and G. Buist. 2018. Human antibody responses against non-covalently cell wall-bound Staphylococcus aureus proteins. Sci. Rep. 8:3231.

Treffon, J., D. Block, M. Moche, S. Reiß, S. Fuchs, S. Engelmann, D. Becher, L. Langhanki, A. Mellmann, G. Peters, and B. C. Kahl. 2018. Adaptation of Staphylococcus aureus to the airways of cystic fibrosis patients by up-regulation of superoxide dismutase M and iron-scavanging proteins. J. Infect. Dis. 217:1453-1461.

Engelmann, S. and S. Fuchs. 2018. Proteomic signatures in Staphylococcus aureus. 1841:113-130.

Boedeker, C., M. Schüler, G. Reintjes, O. Jeske, M. van Teeseling, M. Jogler, P. Rast, D. Borchert, D. P. Devos, M. Kucklick, M. Schaffer, R. Kolter, L. van Niftrik, S. Engelmann, R. Amann, M. Rhode, H. Engelhardt, and C. Jogler. 2017. Determining the bacterial cell biology of Planctomycetes. Nat. Comm. 8:14853. 

Engelmann, S., and M. Hecker. 2016. Pathoproteomics of pathogenic microbes. Proteomics Clin. Appl. 10:895-896.

Rabus, R., S. Engelmann, D. Jahn, J. Petersen, D. Schomburg, S. Schulz, and I. Wagner-Döbler. 2016. Systembiologie der marinen Roseobacter-Gruppe. Systembiologie. In press

Zühlke, D., K. Dörries, S. Maaß, J. Bernhardt, J. Muntel, V. Liebscher, K. Riedel, M. Lalk, U. Völker, S. Engelmann, D. Becher, S. Fuchs, and M. Hecker. 2016. Costs of life – dynamics of the protein inventory of Staphylococcus aureus during long-term anaerobiosis. Sci. Rep. 6:28172.

Chi, V.H., J. Kolata, S. Stentzel, A. Beyer, M. Gesell-Salazar, L. Steil, J. Pané-Farré, V. Rühmling, S. Engelmann, F. Götz, J. M. van Dijl, M. Hecker, U. Mäder, F. Schmidt, U. Völker, and B. M. Bröker. 2016. Adaptive immune response to lipoproteins of S. aureus in healthy subjects. Proteomics 16:2667-2677.

Engelmann, S., S. Fuchs, and M. Hecker. 2016 Physiological proteomics of S. aureus – from the protein inventory to stress physiology and in vivo adaptation. In: G. A. Somerville (ed) Staphylococcus: Genetics and Physiology. Caister Academic Press. Chapter 7, pp119-132.

Stentzel, S., A. Teufelberger, M. Nordengrün, J. Kolata, F. Schmidt, K. van Crombuggen, S. Michalik, J. Kumpfmüller, S. Tischer, T. Schweder, M. Hecker, S. Engelmann, U. Völker, O. Krysko, C. Bachert, and Barbara M. Bröker. 2017. Staphylococcal serine protease-like proteins are pacemakers of allergic airway reactions to Staphylococcus aureus. J. Allerg. Clin. Immunol. 139:492-500

Selle, M., T. Hertlein, B. Oesterreich, T. Klemm, P. Kloppot, E. Müller, R. Ehricht, S. Stentzel, B. M. Bröker, S. Engelmann, and K. Ohlsen. 2016. Global antibody response to Staphylococcus aureus live-cell vaccination. Sci. Rep. 6:24754.

Bauer, I., J. Günther, T. T. Wheeler, S. Engelmann, and H.-M. Seyfert. 2015. Extracellular milieu grossly alters pathogen-specific immune response of mammary epithelial cells. BMC Vet. Res. 11:172.

Stentzel, S., N. Sundaramoorthy, S. Michalik, M. Nordengrün, S. Schulz, J. Kolata, P. Kloppot, S. Engelmann, L. Steil, M. Hecker, F. Schmidt, U. Völker, M.C. Roghmann, and B.M. Bröker. 2015. Specific serum IgG at diagnosis of Staphylococcus aureus blood stream invasion is correlated with disease progression. J. Proteomics 128:1-7.

Kloppot, P., M. Selle, C. Kohler, S. Stentzel, S. Fuchs, V. Liebscher, E. Müller, D. Kale, K. Ohlsen, B. M. Bröker, P. Zipfel, B. Kahl, R. Ehricht, M. Hecker, and S. Engelmann. 2015. Microarray based identification of human antibodies against Staphylococcus aureus antigens. Proteomics Clin. Appl. 9:1003-1011.

Neumann, Y., K. Ohlsen, S. Engelmann, H. Kusch, D. Albrecht, M. Cartron, A. Hurd, and S. Foster. 2015. The effect of skin fatty acids on Staphylococcus aureus. Arch. Microbiol. 197:245-267.

Halbedel, S., S. Reiss, B. Hahn, D. Albrecht, T. Hain, S. Engelmann, and A. Flieger. 2014. A systematic proteomic analysis of Listeria monocytogenes house-keeping protein secretion systems. Mol. Cell. Proteomics 13:3063-3081.

Kusch, H., and S. Engelmann. 2014. The secrets of the secretome in Staphylococcus aureus. Int. J. Med. Microbiol. 304:133-141.

Michaux, C., A. Hartke, C. Martini, S. Reiss, D. Albrecht, A. Budin-Verneuil, M. Sanguinetti, S. Engelmann, T. Hain, N. Vernieul, and J. C. Giard. 2014. Involvement of Enterococcus faecalis sRNAs in the stress response and virulence. Infect. Immun. 82:3599-3611

Müller, M., S. Reiß, R. Schlüter, U. Mäder, A. Beyer, W. Reiß, J. Marles-Wright, R. L. Lewis, H. Pförtner, U. Völker, K. Riedel, M. Hecker, S. Engelmann, and J. Pané-Farré. 2014. The DUF322 protein Asp23 of Staphylococcus aureus is membrane localized and functionally linked to cell wall stress. Mol. Microbiol. 93:1259-1268.

Dangel, A., N. Ackermann, O. Abdel-Hadi, R. Maier, K. Önder, P. Francoise, R. Benz, C. W. Mueller, J. Pané-Farré, S. Engelmann, J. Schrenzel, J. Heesemann, and C. Lindermayr. 2013. A de-novo designed antimicrobial peptide with activity against multi-resistant Staphylococcus aureus acting on RsbW kinase. FASEB J. 27:4476-4488.

Fuchs, S., D. Zühlke, J. Pané-Farré, H. Kusch, C. Wolf, S. Reiß, L. T. N. Binh, D., Albrecht, K. Riedel, M. Hecker, and S. Engelmann. 2013. Aureolib – a proteome signature library: Towards an understanding of Staphylococcus aureus pathophysiology. PlosOne. 8:e70669.

Reiß, S., J. Pané-Farré, S. Fuchs, P. Franҫois, M. Liebeke, J. Schrenzel, U. Lindequist, M. Lalk, C. Wolz, M. Hecker, and S. Engelmann. 2012. Global analysis of the Staphylococcus aureus response to mupirocin. Antimicrob. Agents Chemother. 56:787-804.

Kusch, K., K. Hanke, S. Holtfreter, M. Schmudde, C. Kohler, C. Erck, J. Wehland, M. Hecker, K. Ohlsen, B. Bröker, and S. Engelmann. 2011. The influence of SaeRS and σB on the expression of superantigens in different Staphylococcus aureus isolates. Int. J. Med. Microbiol. 301:488-499.

Wolf, C., H. Kusch, S. Monecke, D. Albrecht, S. Holtfreter, C. von Eiff, W. Petzl, P. Rainard, B. Bröker, and S. Engelmann. 2011. Genomic and proteomic characterization of Staphylococcus aureus mastitis isolates of bovine origin. Proteomics 11:2491-2502.

Liang, C., M. Liebeke, R. Schwarz, D. Zühlke, S. Fuchs, L. Menschner, S. Engelmann, C. Wolz, S. Jaglitz, J. Bernhardt, M. Hecker, M. Lalk, and T. Dandekar. 2011. S. aureus physiological growth limitations: Insights from flux calculations built on proteomics and external metabolite data. Proteomics 11:1915-1935.

Liebeke, M., K. Dörries, D. Zühlke, J. Bernhardt, S. Fuchs, J. Pané-Farré, S. Engelmann, U. Völker, R. Bose, T. Dandekar, U. Lindequist, M. Hecker, and M. Lalk. 2011. A metabolomics and proteomics study of the adaptation of Staphylococcus aureus to glucose starvation. Mol. BioSystems 7:1241-1253.

Kolata, J., L. G. M. Bode, S. Holtfreter, L. Steil, H. Kusch, B. Holtfreter, M. Hecker, S. Engelmann, A. van Belkum, U. Völker, and B. M. Bröker. 2011. Distinctive patterns in the human antibody response to Staphylococcus aureus bacteremia in carriers and non-carriers. Proteomics 11:3914-3927.

Quiel, A., B. Jürgen, G. Piechotta, A.-P. Le Foll, A.-K. Ziebandt, C. Kohler, D. Köster, S. Engelmann, C. Erck, R. Hintsche, J. Wehland, M. Hecker, and T. Schweder. 2010. Electrical protein array chips for the detection of staphylococcal virulence factors. Appl. Microbiol. Biotech. 85:1619-1627.

Chlebowicz, M.A., K. Nganou, S. Kozytska, J.P. Arends, S. Engelmann, H. Grundmann, K. Ohlsen, J.M. van Dijl, and G. Buist. 2010. Recombination between ccrC in a type V (5C2&8) staphylococcal cassette chromosome mec (SCCmec) of Staphylococcus aureus ST398 leads to conversion from methicillin resistance to methicillin susceptibility in vivo. Antimicrob. Agents Chemother. 54:783-791.

Ziebandt, A. K., H. Kusch, M. Degner, S. Jaglitz, M.J.J.B. Sibbald, J. P. Arends, M. A. Chlebowicz, D. Albrecht, R. Pantuček, J. Doškar, W. Ziebuhr, B. M. Bröker, M. Hecker, J. M. van Dijl, and S. Engelmann. 2010. Proteomics uncovers extreme heterogenity in the Staphylococcus aureus exoproteome due to genomic plasticity and variant gene regulation. Proteomics 10:1634-1644.

Pagels, M., S. Fuchs, J. Pané-Farré, C. Kohler, L. Menschner, M. Hecker, P. J. McNamarra, M. C. Bauer, C. von Wachenfeldt, M. Liebeke, M. Lalk, G. Sander, C. von Eiff, R. A. Proctor, and S. Engelmann. 2010. Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus. Mol. Microbiol. 76:1142-1161.

Sibbald, M.J., T. Winter, M.M. van der Kooi-Pol, G. Buist, E. Tsompanidou, T. Bosma, T. Schäfer, K. Ohlsen, M. Hecker, H. Antelmann, S. Engelmann, and J.M. van Dijl. 2010. Synthetic effects of secG and secY2 mutations on exoproteome biogenesis in Staphylococcus aureus. J. Bacteriol. 192:3788-3800.

Lam, T.T., B. Giese, D. Chikkaballi, A. Kühn, W. Wolber, J. Pané-Farré, D. Schäfer, S. Engelmann, M. Fraunholz, and B. Sinha. 2010. Phago-lysosomal integrity is generally maintained after Staphylococcus aureus invasion of non-professional phagocytes, but is modulated by strain 6850. Infect. Immun. 78:3392-3403.

Fuchs, S., H. Mehlan, H. Kusch, A. Teumer, D. Zühlke, M. Berth, C. Wolf, T. Dandekar, M. Hecker, S. Engelmann, and J. Bernhardt. 2010. Protecs, a comprehensive and powerful storage and analysis system for OMICS data, applied for profiling the anaerobiosis response of Staphylococcus aureus COL. Proteomics 10:2982-3000.

Liang, C., C. Wolz, S. Herbert, J. Bernhardt, S. Engelmann, M. Hecker, F. Götz, and T. Dandekar. 2009. GENOVA: A rapid genome visualization and functional genomics software. Online J. Bioinform. 10:201-207.

Eichstaedt, S., K. Gäbler, S. Below, C. Müller, C. Kohler, S. Engelmann, P. Hildebrandt, U. Völker, M. Hecker, and J. P. Hildebrandt. 2009. Effects of Staphylocccus aureus-hemolysin A on calcium signalling in immortalized human airway epithelial cells. Cell Calcium. 45:165-176.

Chatterjee, I., S. Schmitt, C. F. Batzilla, S. Engelmann, A. Keller, M. W. Ring, R. Kautenburger, W. Ziebuhr, M. Hecker, K. T. Preissner, M. Bischoff, R. A. Proctor, H. P. Beck, H. P. Lenhof, G. A. Sommerville, and M. Herrmann. 2009. Staphylococcus aureus ClpC ATPase is a late growth phase effector of metabolism and persistence. Proteomics 9:1152-1176.

Below, S., A. Konkel, C. Zeeck, C. Müller, C. Kohler, S. Engelmann, and J. P. Hildebrandt. 2009. Virulence factors of Staphylococcus aureus induce Erk-MAP kinase activation and c-Fos expression in S9 and 16HBE 14o-human airway epithelial cells. Am. J. Physiol. Lung Cell Mol. Physiol. 296:L470-479.

Pané-Farré, J., B. Jonas, S. W. Hardwick, K. Gronau, R. J. Lewis, M. Hecker, and S. Engelmann. 2009. The role of RsbU in controlling SigB-activity in Staphylococcus aureus following alkaline stress. J. Bacteriol. 191:2561-2573.

Eberhardt, C., S. Engelmann, H. Kusch, D. Albrecht, M. Hecker, I. B. Authenrieth, and V. A. J. Kempf. 2009. Proteomic analysis of the bacterial pathogen Bartonella henselae and identification of immunogenic proteins for serodiagnosis. Proteomics 9:1967-1981

Seidl, K., S. Müller, P. Francoise, C. Kriebitzsch, J. Schrenzel, S. Engelmann, M. Bischoff, and B. Berger-Bächi. 2009. Effect of a glucose impulse on the CcpA regulon in Staphylococcus aureus. BMC Microbiol. 18:95.

Shevchuk, O., C. Batzilla, S. Hägele, H. Kusch, S. Engelmann, M. Hecker, A. Haas, K. Heuner, G. Glöckner, and M. Steiner. 2009. Proteomic analysis of Legionella-containing phagosomes isolated from Dictyostelium. Int. J. Med. Microbiol. 299:489-508.

Holtfreter, S., T. T. Nguyen, H. Wertheim, L. Steil, H. Kusch, Q. P. Truong, S. Engelmann, M. Hecker, U. Völker, A. van Belkum, and B. M. Bröker. 2009. Human immune proteome in experimental colonization with Staphylococcus aureus. Clin. Vaccine Immunol. 16:1607-1614.

Schäfer, D., T.T. Lam, T. Geiger, M. Mainiero, S. Engelmann, M. Hussain, , A. Bosserhoff, M. Frosch, M. Bischoff, C. Wolz, J. Reidl, and B. Sinha. 2009. A point mutation in the sensor histidine kinase SaeS of Staphylococcus aureus strain Newman alters response to biocide exposure. J. Bacteriol. 191:7306-7314.

Becher, D., K. Hempel, S. Sievers, D. Zühlke, J. Pané-Farré, A. Otto, S. Fuchs, D. Albrecht, J. Bernhardt, S. Engelmann, U. Völker, J. M. van Dijl, and M. Hecker. 2009. A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome. PLoS One 4:e8176.

Kusch, H., S. Engelmann, R. Bode, D. Albrecht, J. Morschhäuser, and M. Hecker. 2008. A proteomic view of Candida albicans yeast cell metabolism in exponential and stationary growth phase. Int. J. Med. Microbiol. 298:291-318.

Lorenz, U., C. Hüttinger, T. Schäfer, W. Ziebuhr, A. Thiede, J. Hacker, S. Engelmann, M. Hecker, and K. Ohlsen. 2008. The alternative sigma factor sigmaB of Staphylococcus aureus modulates virulence in experimental central venous catheter-related infections. Mircobes Infect. 10:217-223.

Hain, T., H. Hossain, S.S. Chatterjee, S. Machata, U. Volk, S. Wagner, B. Brors, S. Haas, C.T. Kuenne, A. Billion, S. Otten, J. Pané-Farré, S. Engelmann, and T. Chakraborty. 2008. Temporal transcriptomic analysis oft he Listeria monocytogenes EGD-e sigmaB regulon. BMC Microbiol. 8:20.

Galka, F., S.N. Wai, H. Kusch, S. Engelmann, M. Hecker, B. Schmeck. S. Hippenstiel, B.E. Uhlin, and M. Steinert. 2008. Proteomic characterization of the whole secretome of Legionella pneumophila and functional analysis of outer membrane vesicles. Infect. Immun. 76:1825-1836.

Wolf, C., F. Hochgräfe, H. Kusch, D. Albrecht, M. Hecker, and S. Engelmann. 2008. Proteomic analysis of antioxidant strategies of Staphylococcus aureus: Diverse responses to different oxidants. Proteomics 8:3139-3153.

Hochgräfe, F., C. Wolf, S. Fuchs, M. Liebeke, M. Lalk, S. Engelmann, and M. Hecker. 2008. Nitric oxide stress induces different responses but mediates comparable protein thiol protection in Bacillus subtilis and Staphylococcus aureus. J. Bacteriol. 190:4997-5008.

Kohler, C., C. von Eiff, M. Liebeke, P.J. McNamara, M. Lalk, R.A. Proctor, M. Hecker, and S. Engelmann. 2008. A defect in menadione biosynthesis induces global changes in gene expression in Staphylococcus aureus. J. Bacteriol. 190:6351-6364.

Grumann, D., S.S. Scharf, S. Holtfreter, C. Kohler, L. Steil, S. Engelmann, M. Hecker, U. Völker, and B.M. Bröker. 2008. Immune cell activation by enterotoxin gene cluster (egc)-encoded and non-egc superantigens from Staphylococcus aureus. J. Immunol. 181:5054-5061.

Schlag, S., S. Fuchs, C. Nerz, R. Gaupp, S. Engelmann, M. Liebeke, M. Lalk, M. Hecker, and F. Götz. 2008. Characterization of oxygen-responsive NreABC regulon of Staphylococcus aureus. J. Bacteriol. 190:7847-7858.

Nagy, G., T. Palkovics, A. Otto, H. Kusch, B. Kocsis, U. Dobrindt, S. Engelmann, M. Hecker, L. Emödy, T. Pál, and J. Hacker. 2008. „Gently rough“: vaccine potential of a Salmonella enterica lipopolysaccharide regulatory mutant. J. Infect. Dis. 198:1699-1706.

Kusch, H., S. Engelmann, D. Albrecht, J. Morschhäuser, and M. Hecker. 2007. Proteomic analysis of oxidative stress response in Candida albicans. Proteomics 7:686-697.

Fuchs, S., J. Pané-Farré, C. Kohler, M. Hecker, and S. Engelmann. 2007. Anaerobic gene regulation in Staphylococcus aureus. J. Bacteriol. 189:4275-4289.

Halbedel, S., H. Eilers, B. Jonas, J. Busse, M. Hecker, S. Engelmann, and J. Stülke. 2007. Transcription in Mycoplasma pneumoniae: Analysis of the promoters of the ackA and ldh genes. J. Mol. Biol. 371:596-607.

Makhlin, J., T. Kofman, I. Borovok, C. Kohler, S. Engelmann, G. Cohen, and Y. Aharonovitz. 2007. Staphylococcus aureus ArcR controls expression of the arginine deiminase operon. J. Bacteriol. 189:5976-5986.

Holtfreter, S., K. Roschack, P. Eichler, K. Eske, B. Holtfreter, C. Kohler, S. Engelmann, M. Hecker, A. Greinacher, and B. Bröker. 2006. Staphylococcus aureus carriers neutralize superantigens by antibodies specific for their colonizing strain, a potential explanation for their improved prognosis in severe sepsis. J. Infect. Dis. 193:1275-1278.

Pané-Farré, J., B. Jonas, K. Förstner, S. Engelmann, and M. Hecker. 2006. The σB-regulon in Staphylococcus aureus and its regulation. Int. J. Med. Microbiol. 296:237-258.

Batzilla, C., S. Rachid, S. Engelmann, M. Hecker, J. Hacker, and W. Ziebuhr. 2006. Impact of the accessory gene regulatory system (Agr) on extracellular proteins, codY expression and amino acid metabolism in Staphylococcus epidermidis. Proteomics 6:3602-3613.

Pamp, S. J., D. Frees, S. Engelmann, M. Hecker, and H. Ingmer. 2006. Spx is a global effector impacting stress tolerance and biofilm formation in Staphylococcus aureus. J. Bacteriol. 188:4861-4870.

Rogasch, K., V. Rühmling, J. Pané-Farré, D. Höper, C. Weinberg, S. Fuchs, M. Schmudde, B. M. Bröker, C. Wolz, M. Hecker, and S. Engelmann. 2006. The influence of the two component system SaeRS on global gene expression in two different Staphylococcus aureus strains. J. Bacteriol. 188:7742-7758.

Kohler, C., S. Wolff, D. Albrecht, S. Fuchs, D. Becher, K. Büttner, S. Engelmann, and M. Hecker. 2005. Proteome analyses of Staphylococcus aureus in growing and non-growing cells: A physiological approach. Int. J. Med. Microbiol. 295:547-565.

Holtfreter, S., K. Bauer, D. Thomas, C. Feig, V. Lorenz, K. Roschack, E. Friebe, K. Selleng, S. Lövenich, T. Greve, A. Greinacher, B. Panzig, S. Engelmann, G. Lina, and B. M. Bröker. 2004. egc-encoded superantigens from Staphylococcus aureus are neutralized by human sera much less efficiently than are classical staphylococcal enterotoxins or toxic shock syndrome toxin. Infect. Immun. 72:4061-4071.

Kusch, H., K. Biswas, S. Schwanfelder, S. Engelmann, P. D. Rogers, M. Hecker, and J. Morschhäuser. 2004. A proteomic approach to understanding the development of multidrug-resistant Candida albicans strains. Mol. Genet. Genomics 271:554-565.

Sender, U., J. Bandow, S. Engelmann, U. Lindequist, and M. Hecker. 2004. Proteomic signatures for daunomycin and adriamycin in Bacillus subtilis. Pharmazie 59:65-70.

Weber, H., S. Engelmann, D. Becher, and M. Hecker. 2004. Oxidative stress triggers thiol oxidation in the glyceraldehyde-3-phosphate dehydrogenase of Staphylococcus aureus. Mol. Microbiol. 52:133-140.

Allenby, N. E., N. O'Connor, Z. Pragai, N. M. Carter, M. Miethke, S. Engelmann, M. Hecker, A. Wipat, A. C. Ward, and C. R. Harwood. 2004. Post-transcriptional regulation of the Bacillus subtilis pst operon encoding a phosphate-specific ABC transporter. Microbiology  150:2619-2628.

Ziebandt, A.K., D. Becher, K. Ohlsen, J. Hacker, M. Hecker, and S. Engelmann. 2004. The influence of agr and σB in growth phase dependent regulation of virulence factors in Staphylococcus aureus. Proteomics 4:3034-3047.

Kohler, C., C. von Eiff, G. Peters, R. A. Proctor, M. Hecker, and S. Engelmann. 2003. Physiological characterization of a heme-deficient mutant of Staphylococcus aureus by a proteomic approach. J. Bacteriol. 185:6928-6937.

Giachino, P., S. Engelmann, and M. Bischoff. 2001. Sigma(B) activity depends on RsbU in Staphylococcus aureus. J. Bacteriol. 183:1843-1852.

Ziebandt, A. K., H. Weber, J. Rudolph, R. Schmid, D. Höper, S. Engelmann, and M. Hecker. 2001. Extracellular proteins of Staphylococcus aureus and the role of SarA and sigma B. Proteomics 1:480-493.

Gertz, S., S. Engelmann, R. Schmid, A. K. Ziebandt, K. Tischer, C. Scharf, J. Hacker, and M. Hecker. 2000. Characterization of the sigma(B) regulon in Staphylococcus aureus. J. Bacteriol. 182:6983-6991.

Gertz, S., S. Engelmann, R. Schmid, K. Ohlsen, J. Hacker, and M. Hecker. 1999. Regulation of sigmaB-dependent transcription of sigB and asp23 in two different Staphylococcus aureus strains. Mol. Gen. Genet. 261:558-566.

Petersohn, A., S. Engelmann, P. Setlow, and M. Hecker. 1999. The katX gene of Bacillus subtilis is under dual control of sigmaB and sigmaF. Mol. Gen. Genet. 262:173-179.

Mogk, A., A. Völker, S. Engelmann, M. Hecker, W. Schumann, and U. Völker. 1998. Nonnative proteins induce expression of the Bacillus subtilis CIRCE regulon. J. Bacteriol. 180:2895-2900.

Scharf, C., S. Riethdorf, H. Ernst, S. Engelmann, U. Völker, and M. Hecker. 1998. Thioredoxin is an essential protein induced by multiple stresses in Bacillus subtilis. J. Bacteriol. 180:1869-1877.

Antelmann, H., S. Engelmann, R. Schmid, A. Sorokin, A. Lapidus, and M. Hecker. 1997. Expression of a stress- and starvation-induced dps/pexB-homologous gene is controlled by the alternative sigma factor sigmaB in Bacillus subtilis. J. Bacteriol. 179:7251-7256.

Antelmann, H., S. Engelmann, R. Schmid, and M. Hecker. 1996. General and oxidative stress responses in Bacillus subtilis: cloning, expression, and mutation of the alkyl hydroperoxide reductase operon. J. Bacteriol. 178:6571-6578.

Engelmann, S. and M. Hecker. 1996. Impaired oxidative stress resistance of Bacillus subtilis sigB mutants and the role of katA and katE. FEMS Microbiol. Lett. 145:63-69.

Maul, B., U. Völker, S. Riethdorf, S. Engelmann, and M. Hecker. 1995. sigma B-dependent regulation of gsiB in response to multiple stimuli in Bacillus subtilis. Mol. Gen. Genet. 248:114-120.

Engelmann, S., C. Lindner, and M. Hecker. 1995. Cloning, nucleotide sequence, and regulation of katE encoding a sigma B-dependent catalase in Bacillus subtilis. J. Bacteriol. 177:5598-5605.

Völker, U., S. Engelmann, B. Maul, S. Riethdorf, A. Völker, R. Schmid, H. Mach, and M. Hecker. 1994. Analysis of the induction of general stress proteins of Bacillus subtilis. Microbiology 140:741-752.

Riethdorf, S., U. Völker, U. Gerth, A. Winkler, S. Engelmann, and M. Hecker. 1994. Cloning, nucleotide sequence, and expression of the Bacillus subtilis lon gene. J. Bacteriol. 176:6518-6527.

Mraheil, M.A., A. Billion, C. Kuenne, J. Pischimarov, B. Kreikemeyer, S. Engelmann, A. Hartke, J.C. Giard, M. Rupnik, S. Vorwerk, M. Beier, J. Retey, T. Hartsch, A. Jacob, F. Cemic, J. Hemberger, T. Chakraborty, and T. Hain. 2010. Comparative genome-wide analysis of small RNAs of major Gram-positive pathogens: from identification to appplication. Microbial Biotechnol. 3:658-676.

Hecker, M., D. Becher, S. Fuchs, and S. Engelmann. 2010. A proteomic view of cell physiology and virulence of Staphylococcus aureus. Int. J. Med. Microbiol. 300:76-87.

Hecker, M., A. Reder, S. Fuchs, M. Pagels, and S. Engelmann. 2009. Physiological proteomics and stress/starvation responses in Bacillus subtilis and Staphylococccus aureus. Res. Microbiol. 160:245-258.

Becker, K., G. Bierbaum, C. von Eiff, S. Engelmann, F. Götz, J. Hacker, M. Hecker, G. Peters, R. Rosenstein, and W. Ziebuhr. 2007. Understanding the physiology and adaptation of staphylococci: A post genomic approach. Int. J. Med. Microbiol. 297:483-501.

Sibbald, M. J. J. B., A. K. Ziebandt, S. Engelmann, M. Hecker, A. de Jong, H. J. M. Harmsen, G. C. Raangs, I. Stokroos, J. P. Arends, J. Y. F. Dubois, and J. M. van Dijl. 2006. Mapping the pathways to staphylococcal pathogenesis by comparative secretomics. Microbiol. Mol. Biol. Rev. 70:755-788.

Hecker, M., S. Engelmann, and S. J. Cordwell. 2003. Proteomics of Staphylococcus aureus--current state and future challenges. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 787:179-195.

Hecker, M. and S. Engelmann. 2000. Proteomics, DNA arrays and the analysis of still unknown regulons and unknown proteins of Bacillus subtilis and pathogenic gram-positive bacteria. Int. J. Med. Microbiol. 290:123-134.

Engelmann, S. and M. Hecker. 2009. A proteomics view of virulence factors of Staphylococcus aureus. In: H. de Reuse and S. Bereswill (eds.): Microbial Pathogenomics, Karger, Basel, Genome Dyn., volume 6, pp. 187-197.

Engelmann, S. and M. Hecker. 2008. Proteomic analysis to investigate regulatory networks in Staphylococcus aureus. In: F. R. DeLeo and M. Otto (eds.): Bacterial Pathogenesis, Methods and Protocols, Humana Press, Totowa, Chapter 3, pp. 25-45.

Ziebandt, A. K., D. Becher, K. Ohlsen, J. Hacker, M. Hecker, and S. Engelmann. 2007. The influence of agr and sigmaB in growth phase dependent regulation of virulence factors in Staphylococcus aureus. In: P. R. Jungblut and M. Hecker (eds.): Proteomics of microbial pathogens, WILEY-VCH Verlag GmbH Co. KGaA, Weinheim, pp.225-247.

Hecker, M. and S. Engelmann. 2006. Physiological proteomics of Bacillus subtilis and Staphylococcus aureus: Towards a comprehensive understanding of cell physiology and pathogenicity. In: J. Hacker and U. Dobrindt (eds.): Pathogenomics, WILEY-VCH Verlag GmbH Co. KGaA, Weinheim, pp.43-68.