Compound Profiling und Screening

The World Health Organization (WHO) considers the spread of microorganisms which are resistant to the most common antibiotics an increasing threat to the well-being of the world population. Not only is the number of patients increasing whose infectious disease can no longer be treated, but also in some circumstances patients with other severe diseases will not be treated if the therapy is accompanied with suppression of the immune system and thus, an increased risk of infection. Thus, new active agents for the treatment of infectious diseases are urgently needed, as well as the responsible use of existing antibiotics. Prerequisites for the discovery of new drugs are relevant biological screening assays together with compound libraries of broad chemical diversity. We perform primary screens, based either on own protocols or on protocols developed by cooperation partners and transferred to our infrastructure. In cooperation with partners we also perform secondary assays to optimize first primary hits.


Selected Publications

Günther A., Bilitewski U., (1995) Characterisation of inhibitors of acetylcholinesterase by an automated amperometric flow injection system, Anal Chim Acta, 300: 117-125

Mersal GAM, Khodari M, Bilitewski U (2004) Optimisation of the composition of a screen-printed acrylate polymer enzyme layer with respect to an improved selectivityand stability of enzyme electrodes Biosens Bioelectron 20: 305 - 314

Behnsen* J, Narang* P, Hasenberg M, Gunzer F, Bilitewski U, Klippel N, Rohde M, Brock M, Brakhage AA, Gunzer M (2007) The environmental dimensionality controls the interaction of phagocytes with the pathogenic fungi Aspergillus fumigatus and Candida albicans, PLOS Pathog 3: e13

Bilitewski U,* Blodgett JAV, Duhme-Klair AK, Dallavalle S, Laschat S, Routledge A, Schobert R (2017) Chemical and Biological Aspects of Nutritional Immunity – Perspectives for New Anti-infectives Targeting Iron Uptake  Systems Angew Chem Int Ed Engl 56: 14360 - 14382


  • Authors: Cui,S.; Hassan,R.Y.; Heintz-Buschart,A.; Bilitewski,U.; ( Year: 2016). Title: Regulation of Candida albicans Interaction with Macrophages through the Activation of HOG Pathway by Genistein.Journal: Molecules. Volume: 21 Issue: 2, S-Page: article no. 162 DOI: 10.3390/molecules21020162.HZI repositoryPubMed
  • Authors: Bruckner,S.; Bilitewski,U.; Schobert,R.; ( Year: 2016). Title: Synthesis and Antibacterial Activity of Four Stereoisomers of the Spider-Pathogenic Fungus Metabolite Torrubiellone D.Journal: Organic Letters Volume: 18 Issue: 5, S-Page: 1136- E-Page: 1139 PubMed
  • Authors: Kaba,Hani E.J.; Maier,N.; Schliebe-Ohler,N.; Mayer,Y.; Müller,Peter P.; van den Heuvel,Joop; Schuchhardt,J.; Hanack,K.; Bilitewski,Ursula; ( Year: 2015). Title: Identification of whole pathogenic cells by monoclonal antibodies generated against a specific peptide from an immunogenic cell wall protein.Journal: Journal of Microbiological Methods Volume: 108, S-Page: 61- E-Page: 69 WEBURLS
  • Authors: Khodari,M.; Bilitewski,U.; Basry,A.A.H.; ( Year: 2015). Title: Screen-Printed Electrodes for Amperometric Determination of Iodide.Journal: Electroanalysis Volume: 27 Issue: 2, S-Page: 281- E-Page: 284 WEBURLS
  • Authors: Kaba,H.E.J.; Pölderl,A.; Bilitewski,U.; ( Year: 2015). Title: Short Peptides Allowing Preferential Detection of Candida albicans Hyphae.Journal: Anal.Chem. Volume: 87 Issue: 17, S-Page: 8629- E-Page: 8633 WEBURLS
  • Authors: Kempf,K.; Schmitt,F.; Bilitewski,U.; Schobert,R.; ( Year: 2015). Title: Synthesis, stereochemical assignment, and bioactivity of the Penicillium metabolites penicillenols B1 and B2.Journal: Tetrahedron Volume: 71 Issue: 31, S-Page: 5064- E-Page: 5068 WEBURLS
  • Authors: Cui,S.; Bilitewski,Ursula; ( Year: 2014). Title: [Effect of genistein on the TLR and MAPK transduction cascades in lipopolysaccharide -stimulated macrophages].Journal: Xi.Bao.Yu Fen.Zi.Mian.Yi.Xue Za Zhi. Volume: 30 Issue: 3, S-Page: 233- E-Page: 236 PubMed
  • Authors: El-Mowafy,Mohammed; Bahgat,M.M.; Bilitewski,Ursula; ( Year: 2013). Title: Deletion of the HAMP domains from the histidine kinase CaNik1p of Candida albicans or treatment with fungicides activates the MAP kinase Hog1p in S. cerevisiae transformants.Journal: BMC Microbiology Volume: 13 Issue: 1, S-Page: 209 WEBURLS
  • Authors: Hassan,R.Y.A.; Bilitewski,Ursula; ( Year: 2013). Title: Direct electrochemical determination of.Journal: Biosensors and Bioelectronics Volume: 49, S-Page: 192- E-Page: 198 DOI: 10.1016/j.bios.2013.05.015HZI repositoryPubMed
  • Authors: Cui,S.; Wienhoefer,N.; Bilitewski,Ursula; ( Year: 2013). Title: Genistein induces morphology change and G2/M cell cycle arrest by inducing p38 MAPK activation in macrophages.Journal: International Immunopharmacology Volume: 18 Issue: 1, S-Page: 142- E-Page: 150
  • Authors: Heintz-Buschart,A.; Eickhoff,H.; Hohn,E.; Bilitewski,Ursula; ( Year: 2013). Title: Identification of inhibitors of yeast-to-hyphae transition in.Journal: Journal of Biotechnology Volume: 164 Issue: 1, S-Page: 137- E-Page: 142 DOI: 10.1016/j.jbiotec.2012.12.004HZI repositoryPubMed
  • Authors: Cui,Shuna; Qian,J.; Bo,P.; ( Year: 2013). Title: Inhibitive effect on phagocytosis of Candida albicans induced by pretreatment with quercetin via actin cytoskeleton interference.Journal: Journal of Traditional Chinese Medicine Volume: 33 Issue: 6, S-Page: 804- E-Page: 809 WEBURLS
  • Authors: Kaba,H.E.; Nimtz,Manfred; Müller,Peter P.; Bilitewski,Ursula; ( Year: 2013). Title: Involvement of the mitogen activated protein kinase Hog1p in the response of Candida albicans to iron availability.Journal: BMC Microbiology Volume: 13, S-Page: 16 DOI: 10.1186/1471-2180-13-16HZI repositoryPubMed
  • Authors: Buschart,A.; Gremmer,K.; El-Mowafy,M.; van den Heuvel,Joop; Müller,Peter P.; Bilitewski,Ursula; ( Year: 2012). Title: A novel functional assay for fungal histidine kinases group III reveals the role of HAMP domains for fungicide sensitivity.Journal: Journal of Biotechnology Volume: 157 Issue: 1, S-Page: 268- E-Page: 277 PubMed
  • Authors: Buschart,A.; Sachs,S.; Chen,X.; Herglotz,J.; Krause,A.; Reinhold-Hurek,B.; ( Year: 2012). Title: Flagella mediate endophytic competence rather than act as MAMPs in rice-Azoarcus sp. strain BH72 interactions.Journal: Molecular Plant-Microbe Interactions Volume: 25 Issue: 2, S-Page: 191- E-Page: 199
  • Authors: Buschart,A.; Burakowska,A.; Bilitewski,Ursula; ( Year: 2012). Title: The fungicide fludioxonil antagonizes fluconazole activity in the human fungal pathogen.Journal: Journal of Medical Microbiology Volume: 61 Issue: Pt 12, S-Page: 1696- E-Page: 1703 DOI: 10.1099/jmm.0.050963-0HZI repositoryPubMed
  • Authors: Hassan,R.Y.; Bilitewski,Ursula*; ( Year: 2011). Title: A viability assay for.Journal: Analytical biochemistry Volume: 419 Issue: 1, S-Page: 26- E-Page: 32 DOI: 10.1016/j.ab.2011.07.025HZI repositoryPubMed
  • Authors: Koutinas,M.; Kiparissides,A.; Lam,M.-C.; Silva-Rocha,R.; Godinho,Miguel*; de Lorenzo,V.; Martins dos Santos,V.A.P.; Pistikopoulos,E.N.; Mantalaris,A.; ( Year: 2011). Title: Improving the prediction of.Journal: Biochemical Engineering Journal Volume: 55 Issue: 2, S-Page: 108- E-Page: 118 DOI: 10.1016/j.bej.2011.03.012HZI repositoryPubMed
  • Authors: Wesolowski,J; Hassan,R.Y.A.; Reinhardt,K.; Hodde,S.; Bilitewski,Ursula*; ( Year: 2010). Title: Antifungal compounds redirect metabolic pathways in yeasts: Metabolites as indicators of modes of action.Journal: Journal of Applied Microbiology Volume: 108 Issue: 2, S-Page: 462- E-Page: 471
  • Authors: Klippel,N.; Cui,S.; Groebe,L.; Bilitewski,Ursula*; ( Year: 2010). Title: Deletion of the Candida albicans histidine kinase gene CHK1 improves recognition by phagocytes through an increased exposure of cell wall beta-1,3-glucans.Journal: Microbiology Volume: 156 Issue: Pt 11, S-Page: 3432- E-Page: 3444 PubMed
  • Authors: Bilitewski,Ursula*; ( Year: 2009). Title: DNA microarrays: an introduction to the technology.Book Title: Microchip Methods in Diagnostics Editors: Bilitewski,Ursula; (Ed.)., S-Page: 1- E-Page: 14
  • Authors: Probst-Kepper,M.; Geffers,R.; Kroger,A.; Viegas,N.; Erck,C.; Hecht,H.-J.; Lünsdorf,H.; Roubin,R.; Moharregh-Khiabani,D.; Wagner,K.; Ocklenburg,F.; Jeron,A.; Garritsen,H.; Arstila,T.P.; Kekalainen,E.; Balling,R.; Hauser,H.; Buer,J.; Weiss,S.; ( Year: 2009). Title: GARP: a key receptor controlling FOXP3 in human regulatory T cells.Journal: Journal of Cellular and Molecular Medicine Volume: 13 Issue: 9, S-Page: 3343- E-Page: 3357 PubMed
  • Authors: Legrand,N.; Ploss,A.; Balling,Rudi*; Becker,P.D.; Borsotti,C.; Brezillon,N.; Debarry,J.; de,Jong Y.; Deng,H.; Di Santo,J.P.; Eisenbarth,S.; Eynon,E.; Flavell,R.A.; Guzmán,C.A.; Huntington,N.D.; Kremsdorf,D.; Manns,M.P.; Manz,M.G.; Mention,J.J.; Ott,M.; Rathinam,C.; Rice,C.M.; Rongvaux,A.; Stevens,S.; Spits,H.; Strick-Marchand,H.; Takizawa,H.; van Lent,A.U.; Wang,C.; Weijer,K.; Willinger,T.; Ziegler,P.; ( Year: 2009). Title: Humanized mice for modeling human infectious disease: challenges, progress, and outlook.Journal: Cell Host and Microbe Volume: 6 Issue: 1, S-Page: 5- E-Page: 9 DOI: 10.1016/j.chom.2009.06.006HZI repositoryPubMed
  • Authors: He,F.; Balling,Rudi*; Zeng,An-Ping*; ( Year: 2009). Title: Reverse engineering and verification of gene networks: principles, assumptions, and limitations of present methods and future perspectives.Journal: Journal of Biotechnology Volume: 144 Issue: 3, S-Page: 190- E-Page: 203 PubMed
  • Authors: Wesolowski,J.; Hassan,R.Y.; Hodde,S.; Bardroff,C.; Bilitewski,Ursula*; ( Year: 2008). Title: Sensing of oxygen in microtiter plates: a novel tool for screening drugs against pathogenic yeasts.Journal: Analytical and Bioanalytical Chemistry Volume: 391 Issue: 5, S-Page: 1731- E-Page: 1737 PubMed
  • Authors: Bilitewski,Ursula*; ( Year: 2007). Title: Biochemische Methoden in der Wasseranalytik - Stand der Technik und Perspektiven. Teil III: Signalprofile und effektbasierte Analytik.Journal: Vom Wasser - Das Journal Volume: 105 Issue: 4, S-Page: 3- E-Page: 30
  • Authors: Behnsen,J.; Narang,P.; Hasenberg,M.; Gunzer,F.; Bilitewski,Ursula*; Klippel,N.; Rohde,Manfred*; Brock,M.; Brakhage,A.A.; Gunzer,Matthias*; ( Year: 2007). Title: Environmental dimensionality controls the interaction of phagocytes with the pathogenic fungi.Journal: PLoS Pathogens Volume: 3 Issue: 2, S-Page: 0138- E-Page: 0151 DOI: 10.1371/journal.ppat.0030013HZI repositoryPubMed
  • Authors: Klippel,N.; Bilitewski,Ursula*; ( Year: 2007). Title: Phagocytosis assay based on living.Journal: Analytical Letters Volume: 40 Issue: 7, S-Page: 1400- E-Page: 1411 DOI: 10.1080/00032710701327047HZI repository
  • Authors: Bilitewski,U.; ( Year: 2006). Title: Biochemische Methoden in der Wasseranalytik - Stand der Technik und Perspektiven - Teil II: Organismische Tests.Journal: Vom Wasser - Das Journal Volume: 104 Issue: 3, S-Page: 7- E-Page: 19 HZI repository
  • Authors: Bilitewski,U.; ( Year: 2006). Title: Biochemische Methoden in der Wasseranalytik - Stand der Technik und Perspektiven - Teil. I: Molekulare Tests.Journal: Vom Wasser - Das Journal Volume: 104 Issue: 1, S-Page: 3- E-Page: 34 HZI repository
  • Authors: Bilitewski,U.; ( Year: 2006). Title: Protein sensing assay formats and devices.Journal: Annals in Chimica Acta Volume: 568, S-Page: 232- E-Page: 247

Publications before 2005

You can download the publications as a pdf-file.

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