Molecular profiling of memory CD8+ T cells in Listeria monocytogenes infection
Friday lunch seminar
Repetitive Antigen Stimulation Induces Stepwise Transcriptome Diversification but Preserves a Core Signature of Memory CD8+ T Cell Differentiation
Repetitive antigen stimulation by prime-boost vaccination or pathogen reencounter increases memory CD8+ T cell numbers, but the impact on memory CD8+ T cell differentiation is unknown. Here we showed that repetitive antigen stimulations induced accumulation of memory CD8+ T cells with uniform effector memory characteristics. However, genome-wide microarray analyses revealed that each additional antigen challenge resulted in the differential regulation of several hundred new genes in the ensuing memory CD8+ T cell populations and, therefore, in stepwise diversification of CD8+ T cell transcriptomes. Thus, primary and repeatedly stimulated (secondary, tertiary, and quaternary) memory CD8+ T cells differed substantially in their molecular signature while sharing expression of a small group of genes and biological pathways, which may constitute a core signature of memory differentiation. These results reveal the complex regulation of memory CD8+ T cell differentiation and identify potential new molecular targets to dissect the function of memory cells generated by repeated antigen stimulation.
HZI Forum, Room X0.13
Dr. Thomas Wirth, Iowa State University, USA / Hannover Medical School
Prof. Dr. med. Lars Zender, HZI