Minicircle DNA - non-viral vectors supporting viral vector production

Martin Schleef

  • Overview

    It has been shown that by so-called reverse packaging backbone sequences of the ITR containing vector plasmid may be encapsidated during the generation of viral particles. As a result of such events, an AAV mediated transfer of the antibiotics resistance gene instead of the therapeutic gene into the target cells appears to be a potential risk of plasmid derived AAV vectors. This can be overcome by using minicircle DNA for e.g. AAV production.

    An overview on the general structure of minicircle systems, first results on manufacturing of AAV Helper & Packaging minicircles and transfer minicircles, as well as their use in AAV production are presented.

    Date: 04.11.2014, 14:30


    Helmholtz Centre for Infection Research

    Building and room

    Forum X1.04


    Dr. Martin Schleef, 

    PlasmidFactory GmbH & Co. KG


  • Sylvia Richter

    Sylvia Richter

    Secretary of Department Gene Regulation and Differentiation

    +49 531 6181-5001

    +49 531 6181-5002