Minicircle DNA - non-viral vectors supporting viral vector production
It has been shown that by so-called reverse packaging backbone sequences of the ITR containing vector plasmid may be encapsidated during the generation of viral particles. As a result of such events, an AAV mediated transfer of the antibiotics resistance gene instead of the therapeutic gene into the target cells appears to be a potential risk of plasmid derived AAV vectors. This can be overcome by using minicircle DNA for e.g. AAV production.
An overview on the general structure of minicircle systems, first results on manufacturing of AAV Helper & Packaging minicircles and transfer minicircles, as well as their use in AAV production are presented.
Helmholtz Centre for Infection Research
Building and room
Dr. Martin Schleef,
PlasmidFactory GmbH & Co. KG