11.11.2021 10:00

HIPS Talk

“Chemical strategies to fight multi-resistant bacteria”

    Multiresistant bacterial pathogens such as Methicillin-resistant Staphylococcus aureus (MRSA) are responsible for a variety of severe infections that pose a significant threat to global health. To approach this challenge new chemical entities with an unprecedented mode of action are desperately needed. This presentation will cover our latest efforts to identify new anti-bacterial targets and corresponding chemical inhibitors. A proteome mining approach will be presented to identify cofactor-dependent enzymes as novel antibiotic targets. Small molecule cofactor mimics infiltrate the bacterial metabolic machinery leading to their incorporation in PLP-dependent enzymes. Their analysis via mass-spectrometry revealed the function of uncharacterized proteins in important bacterial pathways as well as the mechanism of action of known antibiotics.

    In a separate approach we identified a new compound class that effectively kills MRSA strains. Chemical synthesis of improved derivatives led to the identification of an active molecule with nanomolar potency and suitable metabolic stability. Its mode of action was investigated by affinity based protein profiling (AfBPP). The compound stimulates a signal peptidase correlating with enhanced secretion of extracellular proteins. These included essential cell-wall remodeling enzymes whose dysregulation likely explains the associated antibiotic effects. Strikingly, the compound turned out to be a promising therapeutic candidate as it did not induce in vitro resistance, exhibited optimal per oral bioavailibility and in vivo efficacy in a mouse infection model.

    The event will take place on YouTube

    Date

    11.11.2021 10:00

    Location

    Online on YouTube

    Speaker

    Prof. Dr. Stephan Sieber

    More information:
    https://www.oc2.ch.tum.de
    https://twitter.com/SieberLab
    CV-Download

    Host

    Prof. Dr. Rolf Müller

    Referent: Stephan Sieber

    Portrait of Prof Dr Sieber

    Your referent on this HIPS TALK: Prof Dr Sieber © TU München

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