Masterarbeit

08.03.2019

MSYS

Regulation der Interferon-vermittelten Geninduktion durch Zell-Zell-Kommunikation

Aufgaben

Interferons (IFNs) trigger anti-viral activities in the host cell by inducing a concerted program of IFN-stimulated genes (ISGs). We use fluorescent ISG reporter systems to analyze gene induction at the single cell level. Recent studies in intestinal epithelial cells and mini-gut organoids revealed that ISG induction and acquisition of the antiviral status in a clonal cell populations is bimodal, meaning that only a fraction of cells is protected from infection (Rand et al., MolSysBiol 2012; Bhushal et al., Front Immunol 2017). Ongoing work identified gap junction-mediated communication as a novel strategy that diminish this cell-to-cell heterogeneity in ISG induction. However, the nature of the exchanged signal is still unknown.

 

Ziel:

The aim of this project is to elucidate the molecular basis of this cell-to-cell interaction. We hypothesize that molecules are exchanged between cells via gap-junctions and that this exchange overcomes negative feed-back loops of IFN signaling. Within this project is planned to down-regulate selected negative regulators (at the mRNA level) and miRNAs by siRNAs and antagomirs in cell culture and examine the effect on ISG induction and IFN signal transduction.

 

Methoden:

Proposed work plan includes mammalian cell culture, cultivation of organoids, Western Blot, Immunofluorescence staining, FACS analysis and confocal laser-scanning microscopy. The project includes infection of mammalian cell lines with virus and analysis of gene induction by qRT-PCR.

Standort

Hauptsitz Braunschweig

Forschungsgruppe

Veröffentlicht

08.03.2019

Prof. Dr. Dagmar Wirth
Tel. 0531-6181-5040,
dagmar.wirth@helmholtz-hzi.de 

Die Arbeiten sollen zeitnah beginnen. Weitere Information erhalten Sie bei Dr. Mario Köster Tel. 0531-6181-5091, mario.koester@helmholtz-hzi.de oder Prof. Dr. Dagmar Wirth Tel. 0531-6181-5040, dagmar.wirth@helmholtz-hzi.de und, an die auch die Bewerbungsunterlagen geschickt werden sollen.

Wussten Sie schon?

HZI International Graduate School for Infection Research

Die HZI International Graduate School for Infection Research (GS-FIRE) bietet ein innovatives, strukturiertes Promotionsprogramm im Themenbereich "Infektionsforschung".

PHD-Studiengang Epidemiologie

Der PhD-Studiengang „Epidemiologie“ ist ein dreijähriger Promotionsstudiengang im Bereich der epidemiologischen Forschung und wird von der Abteilung für Epidemiologie des HZI betrieben.

DruckenPer Mail versendenTeilen