Chemische Biologie

Auf der Suche nach neuen Therapien gegen Krankheitserreger setzen Wissenschaftler unter anderem auf chemische und biologische Wirkstoffe. Dies können Verbindungen sein, die als Antibiotikum wirken oder zur Stimulierung des Immunsystems dienen. Neue Wirkstoffe zu entdecken, ihre Funktionsweise zu charakterisieren und ihre Eigenschaften zu optimieren sind die drei Hauptziele der Abteilung „Chemische Biologie“ am HZI.

Team

    Prof. Dr. Peter Paul Müller

    Peter Paul Müller

    Wissenschaftler

    0531 6181-5070

    0531 6181-5002

    Kontakt

     

     


    Lebenslauf

     

     

    Publications of Prof. Dr. Peter Müller

    MedlineGoogleScopus

    Vascular iron implant degradation kinetics, corrosion product localization and transcriptional response in a novel murine model. Peter P. Mueller, Sylvia Arnold, Muhammad Badar, Dirk Bormann, Heinz Haferkamp, Andreas Drynda , Andrea Meyer-Lindenberg, Hansjörg Hauser, Matthias Peuster. Journal of Biomedical Materials Research: Part A. 2012 Accepted.

     

    Reich U, Fadeeva E, Warnecke A, Paasche G, Müller P, Chichkov B, Stöver T, Lenarz T, Reuter G. (2012) Directing neuronal cell growth on implant material surfaces by microstructuring.  Journal of Biomedical Materials Research: Part B - Applied Biomaterials. Pubmed

     

    Böldicke, T., S. Somplatzki, G. Sergeev, P.P. Mueller. (2011) Functional inhibition of transitory proteins by intrabody-mediated retention in the endoplasmatic reticulum. Methods doi:10.1016/j.ymeth.2011.10.008 

     

    Buschart A, Gremmer K, El-Mowafy M, van den Heuvel J, Mueller PP, Bilitewski U. (2012) A novel functional assay for fungal histidine kinases group III reveals the role of HAMP domains for fungicide sensitivity. J Biotechnol. 157, 268-277 Link

     

    Ehlert N., A. Hoffmann, G. Gross, P.P. Mueller, M. Stieve, T. Lenarz, P. Behrens (2011) Amino modified silica surfaces efficiently immobilize Bone Morphogenetic Protein 2 (BMP2) for medical purposes. Acta Biomaterialia 7, 1772-1779 Link
       
    Ehlert N., M. Badar, M. Stieve, T. Lenarz, P.P. Müller, P. Behrens (2011). Mesoporous Silica Coatings for Controlled Release of Ciprofloxacin from Implants. J. Mater. Chem. 21, 752-760 Link

     

    Badar M., K. Hemmen, M. Nimtz, H. Hauser, M. Stieve, M. Stiesch, T. Lenarz, U. Möllmann, S. Vogt, M. Schnabelrauch, P.P. Mueller. (2010). Evaluation of madurahydroxylactone as a slow release antibacterial implant coating. TOBEJ, 4: 263-270. Link

     

    Ehlert N., P.P. Mueller, M. Stieve, P. Behrens (2010). Immobilization of alkaline phosphatase on modified silica coatings. Microporous & Mesoporous Materials 131: 51-57. Link

     

    Mojallal H, M. Stieve, I. Krueger, P. Behrens, P. P. Mueller, T. Lenarz (2009). A Biomechanical Ear Model to Evaluate Middle Ear Reconstruction.  International Journal of Audiology. Int J Audiol. 48(12):876-84. PubMed

     

    Mueller PP, Drynda A, Goltz D, Hoehn R, Hauser H, Peuster M. (2009) Common gene expression signatures in the postnatal arterial duct and in stented arteries. Cardiol Young 19:352-359 PubMed

     

    Stieve M, Hedrich HJ, Battmer RD, Behrens P, Müller P, Lenarz T. (2009) Experimental middle ear surgery in rabbits: a new approach for reconstructing the ossicular chain. Laboratory Animals 43(2):198-204 PubMed

     

    Vogt JC, Brandes G, Ehlert N, Behrens P, Nolte I, Mueller PP, Lenarz T, Stieve M. (2009) Free Bioverit(R) II Implants Coated with a Nanoporous Silica Layer in a Mouse Ear Model - A Histological Study.  J Biomater Appl. 24(2):175-191. PubMed

     

    Stieve M, Hedrich H, Mojallal H, Behrens P, Müller P, Lenarz T. (2008) Normative data of Multifrequency Tympanometry in rabbits. Laboratory Animals 42, 320-325 PubMed 

     

    Reich U, Mueller PP, Fadeeva E, Chichkov BN, Stoever T, Fabian T, Lenarz T, Reuter G. (2008) Differential fine-tuning of cochlear implant material-cell interactions by femtosecond laser microstructuring. J Biomed Mater Res B Appl Biomater. 2008;87B(1):146-153. PubMed
     

     

    Froese N, Schwarzer M, Niedick I, Frischmann U, Köster M, Kröger A, Mueller PP, Nourbakhsh M, Pasche B, Reimann J, Staeheli P, and Hauser H. (2006): Innate Immune Responses in NF-kappaB-repressing factor (NRF)-Deficient Mice. Mol. Cell. Biol. 26(1):293-302. PubMed

     

    Mueller PP, May T, Perz A, Hauser H, Peuster M. (2006): Control of smooth muscle cell proliferation by ferrous iron. Biomaterials 27, 2193–2200. PubMed

     

    Witte F, Reifenrath J, Müller PP, Crostack H-A, Nellesen J, Bach FW, Bormann D, Rudert M. (2006) Cartilage repair on magnesium scaffolds used as a subchondral bone replacement. Mat.-wiss. u. Werkstofftech. 2006, 37, 504-508. Link

     

    Boldicke T, Weber H, Mueller PP, Barleon B, Bernal M. 2005 Novel highly efficient intrabody mediates complete inhibition of cell surface expression of the human vascular endothelial growth factor receptor-2 (VEGFR-2/KDR). J Immunol Methods. 300:146-59. Link 

     
    May T, Wirth D, Hauser H, Mueller PP. 2005 Transcriptionally regulated immortalization overcomes side effects of temperature-sensitive SV40 large T antigen. Biochem Biophys Res Commun. 327:734-41. PubMed

     

    Reich U, Reuter G, Müller P, Stöver T, Lubatschowski H, Lenarz T. 2005 Untersuchung des Zellwachstums auf mikrostrukturierten Elektrodenmaterialien. Biomaterialien 6, 194 Link 

     

    May, T., Müller, P.P., Weich, H., Froese, N., Deutsch, U., Wirth, D., Kröger, A. and Hauser, H. (2005): Establishment of murine cell lines by constitutive and conditional immortalization. J. Biotech. 120, 99-110. PubMed

     

    Samuelsson CV, Lienenklaus S, Muller PP, Zawatzky R, Hauser H, Weiss S. 2005 Transformation of mouse fibroblasts alters the induction pattern of type I IFNs after virus infection. Biochemical and Biophysical Research Community. 335:584-589 PubMed

     

    Niedick I, Froese N, Oumard A, Mueller PP, Nourbakhsh M, Hauser H, Koster M. Nucleolar localization and mobility analysis of the NF-kappaB repressing factor NRF. J Cell Sci. 2004 ;117:3447-58. PubMed 

     

    May, T. Lindenmaier, W. Wirth, D. Mueller, P.P. 2004 Application of a Reversible Immortalization System for the Generation of Proliferation-Controlled Cell Lines. Cytotechnology. 46:69-78 PubMed

     

    Kroger A, Koster M, Schroeder K, Hauser H, Mueller PP. 2002 Activities of IRF-1. J Interferon Cytokine Res. 22:5-14. PubMed

     

    Schroeder K, Koschmieder S, Ottmann OG, Hoelzer D, Hauser H, Mueller PP. (2002) Coordination of cell growth in cocultures by a genetic proliferation control system. Biotechnol Bioeng. 78, 346-52.

     

    Müller, P.; Oumard, A.; Wirth, D.; Kröger, A.; Hauser, H. Polyvalent vectors for coexpression of multiple genes. In Plasmids for Therapy and Vaccination (ed. Schleef, M.) Wiley-VCH, Weinheim, 119-136 (2001)

     

    Schroeder, K.; Koschmieder, S.; Ottmann, O.G.; Hoelzer, D.; Hauser, H.; Müller, P.P. Establishment of a genetic system to coordinate feeder cell growth in cocultures with primary cells.  In Animal Cell Technology: From Target to Market (eds. Lindner-Olsson, E. et al.) Kluwer Academic Publishers, The Netherlands, 2001 :565-567

     

    Geserick C, Bonarius H, Kongerslev L, Hauser H, Mueller PP (2000). Enhanced productivity during controlled proliferation of BHK cells in continuously perfused bioreactors. Biotech. and Bioeng. 69, 266-274. PubMed

     

    Carvalhal, A.V.; J.L. Moreira; P. Mueller; H. Hauser; M.J.T. Carrondo (2000) Manipulation of culture conditions for BHK cell growth inhibition by IRF-1 activation. Cytotechnology 32, 135-145.

     

    Mueller, P.P., Schlenke, P., Nimtz, M., Conradt, H.S. and Hauser, H. (1999). Recombinant glycoprotein product quality in proliferation controlled BHK-21 cells. Biotech. and Bioeng. 65, 529-536. PubMed

     

    Fussenegger, M., Bailey, J. E., Hauser, H. and Müller, P. P (1999). Genetic optimization of recombinant glycoprotein production by mammalian cells. Trends in Biotechnology, 17:35-42.

     

    Mueller PP, Grueter P, Hinnebusch AG, Trachsel H (1998). A ribosomal protein is required for translational regulation of GCN4 mRNA. Evidence for involvement of the ribosome in eIF2 recycling. J Biol Chem 273, 32870-7.

     

    Koloteva N, Muller PP, McCarthy JE. (1997) The position dependence of translational regulation via RNA-RNA and RNA-protein interactions in the 5'-untranslated region of eukaryotic mRNA is a function of the thermodynamic competence of 40 S ribosomes in translational initiation. J Biol Chem. 272, 16531-16539. 

     

    Vasilescu, S., Ptuskina, M., Lintz, B. Muller, P.P. and McCarthy, J.E. (1996) Mutants of eukaryotic initiation factor eIF-4E with altered mRNA cap binding specificity reprogram mRNA selection by ribosomes in Saccharomyces cerevisiae. J. Biol. Chem 271, 7030-7037.

     

    Altmann M, Muller PP, Wittmer B, Ruchti F, Lanker S and Trachsel H. (1993). A Saccharomyces cerevisiae homologue of mammalian translation initiation factor 4B contributes to RNA helicase activity. EMBO J. 12, 3997-4003.

     

    Lanker, S., Müller, P. P., Altmann, M., Goyer, C., Sonenberg, S. and Trachsel, H. (1992). Interactions of the eIF-4F subunits in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 267, 21167-21171.

     

    Lanker, S., Bushman J. L., Hinnebusch, A.G., Trachsel, H. and Mueller, P. P. (1992). Autoregulation of the yeast lysyl-tRNA synthetase gene GCD5/KRS1 by translational and transcriptional control mechanisms. Cell 70, 647-658. PubMed 

     

    Müller, P.P. and Trachsel, H. (1990). Translation and regulation of translation in the yeast Sacchromyces cerevisiae. Eur. J. Biochem. 191, 257-261.

     

    Altmann, M., Müller, P.P., Pelletier, J. Sonnenberg, N. and Trachsel, H. (1989). A mammalian translation initiation factor can substitute for its yeast homologue in vivo. J. Biol. Chem. 264, 12145-12147.

     

    Müller, P.P., Trachsel, H. and Linder, P. (1989). Genetic localization of the Saccharomyces cerevisiae genes TIF1 and TIF2. Curr. Genet. 16, 127-128.

     

    Williams, N.P., Mueller, P.P. and Hinnebusch, A.G. (1988). The positive regulatory function of the 5' proximal open reading frames in GCN4 mRNA can be mimicked by heterologous, short coding sequences. Mol. Cell. Biol. 8, 3827-3836.

     

    Mueller, P.P., Jackson, B.M., Miller, P.F. and Hinnebusch, A.G. (1988). The first and the fourth upstream open reading frames in the GCN4 mRNA have similar initiation efficiencies but respond differently in translational control to changes in length and sequence. Mol. Cell. Biol. 8, 5439-5447. Link

     

    Hinnebusch, A.G., Jackson, B.M. and Mueller, P.P. (1988). Evidence for regulation of reinitiation in translational control of GCN4 mRNA. Proc. Natl. Acad. Sci. USA 85, 7279-7283. 

     

    Mueller, P.P., Harashima, S. and Hinnebusch, A.G. (1987). A segment of GCN4 mRNA containing the upstream AUG codons confers translational control upon a heterologous yeast transcript. Proc. Natl. Acad. Sci. USA 84, 2863-2867.

     

    Mueller, P.P. and Hinnebusch, A.G. (1986). Multiple upstream AUG codons mediate translational control of GCN4. Cell 45, 201-207. PubMed

     

    Costanzo, M.C., Mueller, P.P., Strick, C.A. and Fox, T.D. (1986). Primary structure of wild-type and mutant alleles of the pet494 gene of Saccharomyces cerevisiae. Mol. Gen. Genet. 202, 294-301.

     

    Mueller, P.P. and Fox, T.D. (1984). Molecular cloning and genetic mapping of the PET494 gene of Saccharomyces cerevisiae. Mol. Gen. Genet. 195, 275-280.

     

    Mueller, P.P. Reif, M.K., Shen Zonghou, Sengstang, C. Mason, T.L. and Fox, T.D. (1984). A nuclear yeast mutation, pet494-1, post-transcriptionally blocks accumulation of the mitochondrial gene product cytochrome oxidase subunit III, but can be suppressed by a mitochondrial gene rearrangement. J. Mol. Biol. 175, 431-452.

    Patents:

     

    Mueller PP, Geserick C, Schroeder K, Hauser H (1999). Promoter-transactivator system for inducible high-level mammalian gene expression with the option of cell growth control. Patent EP10467210 A1. International Patent No. PCT/EP 00/03638.

     

    Mueller PP, Hauser H, Steinhilber W, Schäfer K (2001). Novel Recombinant Gene Expression Method. US2006099673 (A1) Link

    Book contributions:

    Kroger A, Koster M, Schroeder K, Mueller PP, Wirth, D, Unsinger J, Hauser H,. (2003) Genetic approaches to recombinant protein production in mammalian cells. Vinci VA, Parekh SR (eds). Handbook of industrial cell culture: Mammalian, microbial and plant cells. Humana Press Inc. Totowa, NJ, 21- 49. 

     

    Kroger A, Koster M, Schroeder K, Hauser H, Mueller PP. (2002) Review: Activities of IRF-1. J Interferon Cytokine Res. 22, 5-14.

     

    Katharina Schroeder, Steffen Koschmieder, Oliver G. Ottmann, Dieter Hoelzer, Hansjörg Hauser and Peter P. Mueller (2001). Establishment of a genetic system to coordinate feeder cell growth in cocultures with primary cells. In: Lindner-Olsson, E., Chatziassavidou, N. and Lüllau, E. (eds). Animal cell technology: from target to market. Kluwer Academic Publishers, 565-567.

     

    Peter P. Müller, André Oumard, Dagmar Wirth, Andrea Kröger and Hansjörg Hauser (2001) Polyvalent vectors for coexpression of multiple genes. Schleef M. (Ed.): Plasmids for Therapy and vaccination. Wiley-VCH, Weinheim, Germany. 

     

    Geserick C, Schroeder K, Bonarius H, Kongerslev L, Schlenke P, Hauser H, Mueller PP (1999). Recombinant pharmaceutical protein overexpression in an IRF-1 proliferation controlled production system. In: Bernard, A., Griffiths, B., Noe W. and Wurm F. (eds.). Animal cell technology: Products from cells, cells as products. Kluwer Academic Publishers, 3-7.

     

    Mueller, P.P., Carvalhal, A.V., Moreira J.L., Geserick, C., Schroeder, K., Carrondo M.J.T. and Hauser, H. (1999). Development of an IRF-1-based proliferation control system. In: Al Rubeai, M. (ed). Cell Engineering 1. Kluwer Academic Publishers, pp. 220-238.

     

    Müller PP, Kirchhoff S, Hauser H. (1998) Sustained expression in proliferation controlled BHK-21 cells. In: Merten O-W, Perrin P, Griffiths B. (eds). New developments and new applications in animal cell technology. Kluwer Academic Publishers, 209-213.

     

    Carvalhal, A.V.; J.L. Moreira; P. Mueller; H. Hauser; M.J.T. Carrondo (1997) "Cell Growth Inhibition by the IRF-1 System". In: O.-W. Merten, P. Perrin and J.B. Griffiths (eds.), "New Developments and New Applications in Animal Cell Technology". Kluwer Academic Publ. pp. 215-217.

     

    Lanker, S., Bushman J. L., Hinnebusch, A.G., Trachsel, H. and Mueller, P. P. (1993). Uncharged tRNA and derepression of general amino acid control: Autoregulation of yeast lysyl-tRNA synthetase. Springer Verlag Berlin, NATO ASI Series, Vol. H71, 143-155.

     

    Müller, P.P., Blum, S., Altmann, M., Lanker, S. and Trachsel, H. (1990). Initiation factors involved in mRNA binding to ribosomes in Sacchromyces cerevisiae. Springer Verlag Berlin, NATO ASI Series, Vol. H49, 487-495.

     

    Hinnebusch, A.G., Mueller, P.P. and Harashima, S. (1988). Translational control of transcriptional activation in the regulation of amino acid biosynthesis in yeast. In Genetics of Translation , Eds. Tuite, M.F. Picard, M., Bolotin-Fukuhara, M. Springer Verlag, Berlin, NATO ASI series 14, 499-512.

     

    Hinnebusch, A.G. and Mueller, P.P. (1987). Translational control of a transcriptional activator in the regulation of amino acid biosynthesis in yeast. In: Translational regulation of gene expression, Edt. Ilan, J., pp. 397-412. Plenum Publishing, New York.

     

     

    Selected Theses

    Etablierung und Evaluierung von molekularbiologischen Testsystemen zur in-vitro und invivo Charakterisierung von neuartigen Implantatmaterialien.  Daniela Lindemeier Diplomarbeit, FH Emden, 2008 Link

    Differentiation of bone marrow stem cells into functional pancreatic insulin-producing cells. Thatava, Tayaramma. Helmholtz-Zentrum für Infektionsforschung (HZI) Braunschweig, 2007 Link

    Auswirkung von Abrasivmitteln zum Wasserabrasivstrahlschneiden auf lebende Zellen. Schulze, Maren; Steiner, Andreas. Medizinische Hochschule Hannover, 2006 Link

     

    Meetings 

    P. Mueller, J. Schöne, N. Ehlert, P. Behrens, S. Leschner, S. Weiss, M. Stieve Imaging of Bacterial Infections Associated with Nanoporous Coated Implants in a Murine Model. Jahrestagung DGBM 2008 Link

    R. Kucharski, D. Bormann, F.-W. Bach, M. Jendras, H. Hauser, P.P. Müller, S. Besdo, J. Reifenrath, A. Meyer-Lindenberg Herstellung von offenporigen Magnesium-Keramik-Implantaten Jahrestagung DGM 2008 Link

    N. Ehlert, P. Behrens, A. Hoffmann, G. Gross, P.P. Müller, T. Lenarz, M. Stieve. Immobilization of the Signalling Protein BMP-2: A Comparison of Mesoporous and Amorphous Silica. 6th International Mesostructured Materials Symposium Mesostructured Materials: Design and Opportunities. Namur, Belgium, 2008. 

    P. Behrens, N. Ehlert, D. Lindemeier, P.P. Müller, G. Brandes, J. Vogt, C. Turck, E. Hlozanek, T. Lenarz, M. Stieve Thorough Testing of Mesoporous Silica as a Biomaterial: Cell Culture, Animal Testing and Gene Expression. 6th International Mesostructured Materials Symposium Mesostructured Materials: Design and Opportunities. Namur, Belgium, 2008

    D. Lindemeier, A. Steiner, M. Stahl, C. Biskup, S. Schmolke, F. Pude, D. Bormann, F. Witte, G. Gross, H. Hauser, A. Hoffmann, P. P. Mueller. Cell Culture Models for the Evaluation of Magnesium Alloys as Degradable Implant Materials.  Jahrestagung DGBM 2007 Biomaterialien, 2007, 8: 189 Link

    J.C. Vogt, M. Stieve, N. Ehlert, D. Lindemeier, G. Brandes, H. Mojallal, T. Lenarz, P. Behrens, P.P. Mueller. Murine Models for the Evaluation of Novel Ossicular Replacement Materials.  Jahrestagung DGBM 2007 Biomaterialien, 2007, 8: 221 Link

    M. Stieve, H.J. Hedrich, H. Mojallal, G. Brandes, R.D. Battmer P. Behrens, P.P.
    Müller,Th. Lenarz. A functional evaluation of ossicular replacement prothesis with different pore size: Effects of conductive hearing loss on auditory brainstem response.  Jahrestagung DGBM 2007 Biomaterialien, 2007, 8: 158 Link

    H. Mojallal, M. Stieve, N. Ehlert, P.P. Mueller, P. Behrens, Th. Lenarz. Funktionelle Evaluierung von Mittelohrprothesen in Kaninchen.  Jahrestagung DGBM 2007 Biomaterialien, 2007, 8: 159 Link

    N. Ehlert, P. Behrens, A. Hoffmann, G. Gross, P.P. Müller, H. Mojallal, J. Vogt, M. Stieve. Immobilisierung von BMP-2 auf amorphem und nanoporösem Siliciumdioxid.  Jahrestagung DGBM 2007 Biomaterialien, 2007, 8: 2192 Link

    M. Stieve, H.J. Hedrich, H. Mojallal, G. Brandes, R.D. Battmer P. Behrens, P.P Müller, Th. Lenarz. Biomaterialien zum Gehörknöchelchenersatz: Ein neuer chirurgischer Zugangswegzur Implantation von Gehörknöchelchenprothesen im Kaninchenmittelohr. Jahrestagung DGBM 2007 Biomaterialien, 2007, 8: 193 Link

    Behrens, P., Müller, PP., Lenarz, T., Besdo S., Mojallal, H., Brandes, G., Hoffman, A., Gross, G., Ehlert, N., Turck, C., Vogt, J., Hlozanek, L., Doniga-Crivat, L., Stieve, M. Solutions for material problems associated with middle ear prostheses.  Jahrestagung DGBM 2007 Biomaterialien, 2007, 8: 261 Link

    W. Heuer, A. Winkel, A. Lutzke, C. Pfaffenroth, H. Menzel, H. Hauser, PP. Müller, WR. Abraham, M. Stiesch-Scholtz,  Biokompatibilitätsuntersuchungen innovativer Implantatbeschichtungenmittels humaner Gingivafibroblasten. Jahrestagung DGBM 2007 Biomaterialien, 2007, 8: 214 Link

    Ehlert, N., Müller, PP., Behrens, P., Immobilization of alkkaline phosphatase on modified glass surfaces. Jahrestagung DGBM 2007, Biomaterialien, 2007, 8: 204 Link

Leitung

  • Prof. Dr. Mark Brönstrup

    Mark Broenstrup

    Abteilungsleiter Chemische Biologie

    0531 6181-3400

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News

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