Intravitale Mikroskopie in Infektion und Immunität

Ob ein infizierter Organismus einen Krankheitserreger erfolgreich bekämpfen kann, hängt in erster Linie von den Wechselwirkungen der Zellen des Immunsystems, sowohl untereinander als auch mit dem Pathogen, ab. Die "Sprache" dieser Wechselwirkungen, lösliche und membrangebundene Signalmoleküle, werden seit einigen Jahren intensiv untersucht. Wie diese Signale im lebenden Organismus ausgetauscht werden, ist noch weitgehend unklar. Mit Hilfe spezieller Mikroskopietechniken lässt sich das Zusammenspiel von Wirt und Pathogen heute aber im lebenden Gewebe verfolgen.

Leitung

Ausgewählte Publikationen

Hapfelmeier S*, Muller AJ*, Stecher B, Kaiser P, Barthel M, Endt K, Eberhard M, Robbiani R, Jacobi CA, Heikenwalder M, Kirschning C, Jung S, Stallmach T, Kremer M, Hardt WD (2008) Microbe sampling by mucosal dendritic cells is a discrete, MyD88-independent step in DeltainvG S. Typhimurium colitis. J Exp Med 205: 437-450 

Muller AJ, Hoffmann C, Galle M, Van Den Broeke A, Heikenwalder M, Falter L, Misselwitz B, Kremer M, Beyaert R, Hardt WD (2009) The S. Typhimurium effector SopE induces caspase-1 activation in stromal cells to initiate gut inflammation. Cell Host Microbe 6: 125-136

Muller AJ*, Filipe-Santos O, Eberl G, Aebischer T, Spath GF, Bousso P* (2012) CD4(+) T Cells Rely on a Cytokine Gradient to Control Intracellular Pathogens beyond Sites of Antigen Presentation. Immunity 37: 147-157 

Muller AJ, Kaiser P, Dittmar KE, Weber TC, Haueter S, Endt K, Songhet P, Zellweger C, Kremer M, Fehling HJ, Hardt WD (2012) Salmonella gut invasion involves TTSS-2-dependent epithelial traversal, basolateral exit, and uptake by epithelium-sampling lamina propria phagocytes. Cell Host Microbe 11: 19-32

Olekhnovitch R, Ryffel B, Muller AJ*, Bousso P* (2014) Collective nitric oxide production provides tissue-wide immunity during Leishmania infection. J Clin Invest 124: 1711-1722 

Publikationen

Müller, A. J. §, S. Aeschlimann, R. Olekhnovitch, M. Dacher, G. F. Späth, P. Bousso§. 2013. Photoconvertible pathogen labeling reveals nitric oxide control of Leishmania major infection in vivo via dampening of parasite metabolism. Cell Host Microbe. 14:460-7. §shared corresponding authors

Felmy, B., P. Songhet, E. Slack, A. J. Müller, M. Kremer, L. Van Maele, D. Cayet, M. Heikenwalder, J.-C. Sirard, W.-D. Hardt. 2013. NADPH oxidase deficient mice develop colitis and bacteremia upon infection with normally avirulent, TTSS-1- and TTSS-2-deficient Salmonella Typhimurium. PLoS One. 8:e77204.

Müller,  A. J. §, O. Filipe-Santos, G. Eberl, T. Aebischer, G. F. Späth, P. Bousso§. 2012. CD4+ T cells rely on bystander effector activity to control local infection. Immunity. 37: 1-11. §shared corresponding authors

Celli, S., M. Day, A. J. Müller, C. Molina-Paris, G. Lythe, P. Bousso. 2012. How many dendritic cells are required to initiate a T cell response? Blood. 120: 3945-8.

Müller,  A. J.*, P. Kaiser*, K. E. J. Dittmar*, T. C. Weber, S. Haueter, K. Endt, P. Songhet, C. Zellweger, M. Kremer, H. J. Fehling, W.-D. Hardt. 2012. Salmonella gut invasion involves TTSS-2-dependent epithelial traversal, basolateral exit, and uptake by epithelium-sampling lamina propria phagocytes. Cell Host Microbe. 11:19-32. *shared first authors

Songhet, P., M. Barthel, B. Stecher, A. J. Müller, M. Kremer, G. C. Hansson, W.-D. Hardt. 2011. Stromal IFN-gamma receptor signaling modulates goblet cell function during Salmonella Typhimurium infection. PLoS One. 6:e22459.

Joller, N., S. S. Weber, A. J. Müller, R. Spörri, P. Selchow, P. Sander, H. Hilbi, A. Oxenius. 2010. Antibodies protect against vacuolar pathogens by Fc Receptor mediated lysosomal targeting. Proc. Natl. Acad. Sci. USA. 107:20441-6.

Lindemann, A., M. Koch, G. Pessi, A. J. Müller, S. Balsiger, H. Hennecke, H.-M. Fischer. 2010. Host-specific symbiotic requirement of BdeAB, a RegR-controlled RND-type efflux system in Bradyrhizobium japonicum. FEMS Microbiol. Lett. 312:184-91.

Endt, K., B. Stecher, S. Chaffron, E. Slack, N. Tchitchek, A. Benecke, L. van Maele, J.-C. Sirard, A. J.  Müller, M. Heikenwalder, A. Macpherson, R. A. Strugnell, C. von Mering, W.-D. Hardt. 2010. The microbiota mediates pathogen clearance from the gut lumen after non-typhoidal Salmonella diarrhea. PLoS Pathog. 6(9): e1001097.

Müller,  A. J., C. Hoffmann, W.-D. Hardt. 2010. Caspase-1 activation via Rho GTPases: A common theme in mucosal infections? PLoS Pathog. 6: e1000795.

Hoffmann, C., M. Galle, S. Dilling, R. Käppeli, A. J. Müller, P. Songhet, R. Beyaert, W.-D. Hardt. 2010. In macrophages, caspase-1 activation by SopE and the Type III Secretion System-1 of S. Typhimurium can proceed in the absence of flagellin. PLoS One.  2010 Aug 30;5(8). pii: e12477.

Müller,  A. J.*, C. Hoffmann*, M. Galle, A. van den Broeke, M. Heikenwalder, L. Falter, B, Misselwitz, M. Kremer, R. Beyaert, W.-D. Hardt. 2009. SopE-induced caspase-1 activation in stromal cells initiates gut inflammation during enteric S. Typhimurium infection. Cell Host Microbe. Aug 20;6:125-36. *shared first authors

Suar, M.,  B. Periaswamy, P. Songhet, B. Misselwitz, A. J. Müller, R. Käppeli, M. Kremer, M. Heikenwalder, W.-D. Hardt. 2009. Accelerated TTSS-2 dependent enteropathogenesis by a serovar Enteritidis PT4/6 strain. Infect. Immun. Sep;77:3569-77.

Müller, A. J.*, S. Hapfelmeier*, B. Stecher, P. Kaiser, M. Barthel, K. Endt, M. Eberhard, R. Robbiani, C.A. Jacobi, M. Heikenwalder, C. Kirschning, S. Jung, T. Stallmach, M. Kremer, W.-D. Hardt.  2008. Microbe sampling by mucosal dendritic cells is a discrete, MyD88-independent step in ∆invG S. Typhimurium colitis. J. Exp. Med. 205:437-50. *shared first authors

Schlumberger, M.C., R. Käppeli, M. Wetter, A. J. Müller, B. Misselwitz, S. Dilling, M. Kremer, and W.-D. Hardt. 2007. Two newly identified SipA domains (F1, F2) steer effector protein localization and contribute to Salmonella host cell manipulation. Mol. Microbiol. 65:741-60.

Schlumberger, M. C., A. J. Müller, K. Ehrbar, B. Winnen, I. Duss, B. Stecher, and W.-D. Hardt. 2005. Real-time imaging of type III secretion: Salmonella SipA injection into host cells. Proc. Natl. Acad. Sci. USA. 102:12548-53.

Hapfelmeier, S., B. Stecher, M. Barthel, M. Kremer, A. J. Müller, M. Heikenwalder, T. Stallmach, K. Pfeffer, S. Akira, and W.-D. Hardt. 2005. The SPI-2 and SPI-1 type III secretion systems allow Salmonella serovar Typhimurium to trigger colitis via MyD88-dependent and MyD88-independent mechanisms. J. Immunol. 174:1675-85.

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