Strukturelle Infektionsbiologie

Die moderne infektionsbiologische Forschung versucht, die Wechselwirkungen zwischen Wirt und Pathogen zu verstehen und gezielt zu manipulieren. Die damit verbundenen Untersuchungen haben interdisziplinären Charakter und verbinden Forschungsfelder wie die Zellbiologie mit der Mikrobiologie. In der Strukturellen Infektionsbiologie werden darüber hinaus verschiedenste biophysikalische Methoden angewendet, die es uns erlauben, den Infektionsprozess oder Teilaspekte davon bei hoher räumlicher und zeitlicher Auflösung zu untersuchen. Diese Abteilung hat ihren Sitz im Center for Structural Systems Biology (CSSB) am Deutschen Elektronen-Synchrotron (DESY) in Hamburg.

Leitung

Ausgewählte Publikationen

Human aryl hydrogen receptor detects virulence factors from bacterial pathogens

Moura-Alves P et al, Aryl hydrogen receptor senses bacterial pigmented virulence factors and orchestrates antibacterial defenses, Nature, 512: 387-392 (2014)

Shigella flexneri type 3 secretion system caught in the act

Dohlich K, Brotcke Zumsteg A, Goosmann C, Kolbe M, A Substrate-Fusion Protein is Trapped inside the Type III Secretion System Channel in Shigella flexneri, PLoS Pathogens, e003881 (2014)

3-dimensional model of the type 3 secretion needle in Salmonella typhimurium

Loquet A Sgourakis NG, Gupta R, Giller K, Riedel D, Goosmann C, Griesinger C, Kolbe* M, Baker D, Becker S, Lange* A, Atomic model of the type III secretion system needle, Nature, 486: 276-279 (2012)

Structure and mechanism of type 3 secretion system needle assembly

Poyraz O, Schmidt H, Seidel K, Delissen F, Ader C, Tenenboim H, Goosmann C, Laube B, Thünemann AF, Zychlinsky A, Baldus M, Lange A, Griesinger C, Kolbe M, Protein refolding is required for assembly of the type three secretion needle. Nat. Struct. Mol. Biol., 17: 788-792 (2010)

Structural based prediction of a conserved effector-chaperone motif for type 3 secretion

Lunelli, M, Lokareddy, RK, Zychlinsky, A, Kolbe, M, IpaB-IpgC interaction defines binding motif for type III secretion translocator. Proc. Natl. Acad. Sci., 106: 9661-9666 (2009)

Publikationen

2019

Wang C, Lunelli M, Zschieschang E, Bosse J, Thuenauer R, Kolbe M (2019) Role of flagellar hydrogen bonding in Salmonella motility and flagellar polymorphic transition. Mol Microbiol: ahead of print doi: 10.1111/mmi.14377

Bernal I, Römermann J, Flacht L, Lunelli M, Uetrecht C, Kolbe M (2019) Structural analysis of ligand-bound states of the Salmonella type III secretion system ATPase InvC. Protein Sci 2019: 1-14. doi: 10.1002/pro.3704

Bernal I, Börnicke J, Heidemann J, Svergun D, Horstmann JA, Erhardt M, Tuukkanen A, Uetrecht C, Kolbe M (2019) Molecular organization of soluble type III secretion system sorting platform complexes. J Mol Biol pii: S0022-2836(19)30425-5    doi: 10.1016/j.jmb.2019.07.004

Liu H, Moura-Alves P, Pei G, Mollenkopf H-J, Hurwitz R, Wu X, Wang F, Liu S, Ma M, Fei Y, Zhu C, Koehler A-B, Oberbeck-Mueller D, Hahnke K, Klemm M, guhlich-Bornhof U, Ge B, Tuukkanen A, Kolbe M, Dorhoi A, Kaufmann SHE (2019) cGAS facilitates sensing of extracellular cyclic dinucleotides to activate innate immunity. EMBO Rep 20: e46293  doi: 10.15252/embr.201846293

2017

Horstmann J A, Zschieschang E, de Diego J, Lunelli M, Rohde M, May T, Strowig T, Stradal T, Kolbe M, Erhardt M (2017) Flagellin phasedependent swimming on epithelial cell surfaces contributes to productive Salmonella gut colonisation. Cell Microbiol. e12739.

2016

Senerovic L, Brotcke-Zumsteg A, Kolbe M (2016) IpaB ion channels induce pyroptosis in macrophages, Shigella: Molecular and Cellular Biology, edited by Bill Picking.

2014

Moura-Alves P, Faé K, Houthuys E, Dorhoi A, Kreuchwig A, Furkert J, Barison N, Diehl A, Munder A, Constant P, Skrahina T, Guhlich-Bornhof U, Klemm M, Koehler A B, Bandermann S, Goosmann C, Mollenkopf H J, Hurwitz R, Brinkmann V, Fillatreau S, Daffe M, Tümmler B, Kolbe M, Oschkinat H, Krause G, Kaufmann S H (2014) AhR sensing of bacterial pigments regulates antibacterial defence. Nature, 512: 387-92.

Dohlich K, Brotcke-Zumsteg A, Goosmann C, Kolbe M (2014) A Substrate-Fusion Protein is Trapped inside the Type III Secretion System Channel in Shigella flexneri. PLoS Pathogens, e003881.

2013

Barison N, Gupta R, Kolbe M (2013) A sophisticated multi-step secretion mechanism: how the type 3 secretion system is regulated. Cellular Microbiology, 15: 1809-1817.

Demers J P, Sgourakis N G, Gupta R, Loquet A, Giller K, Riedel D, Laube B, Kolbe M, Baker D, Becker S, Lange A (2013) The Common Structural Architecture of Shigella flexneri and Salmonella typhimurium Type Three Secretion Needles. PLoS Pathogens, e100324.

2012

Senerovic L, Tsunoda SP, Gossmann C, Brinkmann V, Zychlinsky A, Meissner F, Kolbe M (2012) Spontaneous formation of IpaB ion channels in host cell membranes reveals how Shigella induces pyroptosis in macrophages. Cell Death & Disease, e384.

Mounier J, Boncompain G, Senerovic L, Lagache T, Chrétien F, Perez F, Kolbe M, Olivo-Marin JC, Sansonetti P J, Sauvonnet N (2012) Shigella Effector IpaB-Induced Cholesterol Relocation Disrupts the Golgi Complex and Recycling Network to Inhibit Host Cell Secretion. Cell Host & Microbe, 12: 381-389.

Loquet A, Sgourakis N G, Gupta R, Giller K, Riedel D, Goosmann C, Griesinger C, Kolbe M, Baker D, Becker S, Lange A (2012) Atomic model of the type III secretion system needle. Nature, 486: 276-279

Barison N, Lambers J, Hurwitz R, Kolbe M (2012) Interaction of MxiG with the Cytosolic Complex of the Type III Secretion System Controls Shigella Virulence. FASEB J, 26:1717-1726.

2011

Lunelli M, Hurwitz R, Lambers J, Kolbe M (2011) Crystal Structure of SipD-PrgI: Insight into the Open State of the Type Three Secretion System Needle Tip and its Interaction with Host Ligands. PLoS Pathogens, 7: 1-12.

2010

Lokareddy R K, Lunelli M, Eilers B, Wolter V, Kolbe M (2010) Combination of Two Separate Binding Domains Defines Stoichiometry between Type III Secretion System Chaperone IpgC and Translocator Protein IpaB. J. Biol. Chem., 285: 39965-39975.

Poyraz O, Schmidt H, Seidel K, Delissen F, Ader C, Tenenboim H, Goosmann C, Laube B, Thünemann A F, Zychlinsky A, Baldus M, Lange A, Griesinger C, Kolbe M (2010) Protein refolding is required for assembly of the type three secretion needle. Nat. Struct. Mol. Biol., 17: 788-792.

2009

Lunelli M, Lokareddy R K, Zychlinsky A, Kolbe M (2009) IpaB-IpgC interaction defines binding motif for type III secretion translocator. Proc. Natl. Acad. Sci., 106: 9661-9666.

2008

Averhoff P, Kolbe M, Zychlinsky A, Weinrauch Y (2008) Single residue determines the specificity of neutrophil elastase for Shigella virulence factors. J. Mol. Biol., 377: 1053-1066.

2007

Ernst O P, Gramse V, Kolbe M, Hofmann K P, Heck M (2007) Monomeric G protein-coupled receptor rhodopsin in solution activates its G protein transducin at the diffusion limit. Proc. Natl. Acad. Sci., 104: 10859- 10864.

2006

Blasig I E, Winkler L, Lassowski B, Mueller S L, Zuleger N, Krause E, Krause G, Gast K, Kolbe M, Piontek J (2006) On the self-association potential of transmembrane tight junction proteins. Cell. Mol. Life Sci., 63: 505- 514.

Schubert M, Kolbe M, Kessler B, Oesterhelt D, Schmieder P (2002) Heteronuclear multidimensional NMR spectroscopy of solubilized membrane proteins: resonance assignment of native bacteriorhodopsin. Chembiochem, 4: 1019-1023.

2002

Kolbe M (2002) Röntgenographische und spektroskopische Charakterisierung der lichgetriebenen Ionenpumpe Halorhodopsin aus Halobacterium salinarum.

2000

Paula S, Tittor J, Kolbe M, Oesterhelt D (2000) Spectroscopic studies on crystals from bacterio- and halorhodopsin. Biophys. J., 78: 286.

Kolbe M, Besir H, Essen L O, Oesterhelt D (2000) Structure of the Light- Driven Chloride Pump Halorhodopsin at 1.8 Å Resolution. Science, 288: 1390– 1396

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