Pathogenicity and Biotechnology (3d phase)
New Pseudomonas enzymes acting on 4-substituted but-2-en-4-olides on the third period of the International Research Training Group "Pseudomonas: Pathogenicity and Biotechnology"
AG Microbial Interactions and Processes has been participating since the beginning of this European Graduate
School (EGK) carrying out studies on Pseudomonas sp. strain MT1, a chloroaromatic degrader: In this phase the project is entitled:
New Pseudomonas enzymes acting on 4-substituted but-2-en-4-olides
Background and objectives:
4-Chloromuconolactone (4-carboxymethyl-4-chlorobut-2-en-4-olide) and protoanemonin (4-methylenbut-2-en-4-olide) are key intermediates in a 4-membered community degrading chlorosalicylate by a complex net of metabolic interactions and are subject to transformation by either a new type of hydrolase termed trans-dienelactone hydrolase (acting on 4-chloromuconolactone) or thus far unknown enzymes. The proposed project aims on elucidating the structure, mechanism and substrate specificity of the new type of supposedly metal-dependent hydrolase and on the characterization of the metal centers and on the elucidation of the metabolic pathway and enzymes involved in protoanemonin degradation.
Søren Molin, Michael Givskov, DTU Lyngby, Denmark